Biosynthetic bacterial cellulose graft as arteriovenous fistula and ndash; a complement to existing synthetic grafts?
Introduction: There is an increasing need for vascular prosthesis in dialysis medicine for the construction of arteriovenous (AV)-fistulas for dialysis access. The aims of this study were; a) to develop and validate a new experimental AV-fistula model for larger animals using grafts of bacterial cel...
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| Format: | Article |
| Language: | English |
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GESDAV
2016-06-01
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| Series: | Archives of Clinical and Experimental Surgery |
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| Online Access: | http://www.scopemed.org/fulltextpdf.php?mno=188676 |
| _version_ | 1797913928974467072 |
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| author | Johan Magnusson Katarina Bjorses Jan Holst Martin Malina Karolina Helczynska |
| author_facet | Johan Magnusson Katarina Bjorses Jan Holst Martin Malina Karolina Helczynska |
| author_sort | Johan Magnusson |
| collection | DOAJ |
| description | Introduction: There is an increasing need for vascular prosthesis in dialysis medicine for the construction of arteriovenous (AV)-fistulas for dialysis access. The aims of this study were; a) to develop and validate a new experimental AV-fistula model for larger animals using grafts of bacterial cellulose (BC) between the common carotid artery and the external jugular vein; b) to observe the immediate and intermediate properties (macroscopic and angiographic patency and the macro- and micro thrombogenicity) of the grafts.
Materials and Methods: As graftmaterial bacterial cellulose was used, produced around a preformed scaffold. Bacterial cellulose (BC) is a material produced by the bacteria acetobacter xylinum. A pilotstudy was conducted on 6 pigs to validate the animalmodel and the new graftmaterial. In the following survival study a BC-graft AV-fistula was constructed in 15 pigs.
Results: In the pilot study, 5 out of 6 animals had a patent AV-fistula 4 hours after implantation. In the survival study, after 4 (n3) and 8 (n10) weeks an angiography was performed prior to explantation of the BC-graft. All grafts were occluded with a presumed platelet plug. We conducted an additional acute patch-test comparing the BC and expanded PolyTetraFluoro- Ethylene. A patch of BC and ePTFE was applied to the right and left common femoral artery respectively. At explantation three hours later, all BC-patches showed a thin gel like layer, most likely consisting of platelets, throughout the whole sur- face while the ePTFE-patch showed no, or minimal, signs of platelet adhesions.
Conclusion: Theoretically the cellulose might be similar to autologous veins considering risk of infections and thrombo- genicity. The animal model and the graft material showed good potential in the pilot study. The survival study was discour- aging with the reason for occlusion still to be explained. Bacterial cellulose has a good potential but further development and studies need to be performed. [Arch Clin Exp Surg 2016; 5(2.000): 70-77] |
| first_indexed | 2024-04-10T12:19:04Z |
| format | Article |
| id | doaj.art-8fcfb66441884a53a84bcdadcb92cae8 |
| institution | Directory Open Access Journal |
| issn | 2146-8133 |
| language | English |
| last_indexed | 2024-04-10T12:19:04Z |
| publishDate | 2016-06-01 |
| publisher | GESDAV |
| record_format | Article |
| series | Archives of Clinical and Experimental Surgery |
| spelling | doaj.art-8fcfb66441884a53a84bcdadcb92cae82023-02-15T16:15:33ZengGESDAVArchives of Clinical and Experimental Surgery2146-81332016-06-0152707710.5455/aces.20150907014645188676Biosynthetic bacterial cellulose graft as arteriovenous fistula and ndash; a complement to existing synthetic grafts?Johan Magnusson0Katarina Bjorses1Jan Holst2Martin Malina3Karolina Helczynska4Vascular Center, Skane University Hospital Malmo, Lund University, Malmo, Sweden Vascular Center, Skane University Hospital Malmo, Lund University, Malmo, Sweden Vascular Center, Skane University Hospital Malmo, Lund University, Malmo, Sweden Vascular Center, Skane University Hospital Malmo, Lund University, Malmo, Sweden Vascular Center, Skane University Hospital Malmo, Lund University, Malmo, SwedenIntroduction: There is an increasing need for vascular prosthesis in dialysis medicine for the construction of arteriovenous (AV)-fistulas for dialysis access. The aims of this study were; a) to develop and validate a new experimental AV-fistula model for larger animals using grafts of bacterial cellulose (BC) between the common carotid artery and the external jugular vein; b) to observe the immediate and intermediate properties (macroscopic and angiographic patency and the macro- and micro thrombogenicity) of the grafts. Materials and Methods: As graftmaterial bacterial cellulose was used, produced around a preformed scaffold. Bacterial cellulose (BC) is a material produced by the bacteria acetobacter xylinum. A pilotstudy was conducted on 6 pigs to validate the animalmodel and the new graftmaterial. In the following survival study a BC-graft AV-fistula was constructed in 15 pigs. Results: In the pilot study, 5 out of 6 animals had a patent AV-fistula 4 hours after implantation. In the survival study, after 4 (n3) and 8 (n10) weeks an angiography was performed prior to explantation of the BC-graft. All grafts were occluded with a presumed platelet plug. We conducted an additional acute patch-test comparing the BC and expanded PolyTetraFluoro- Ethylene. A patch of BC and ePTFE was applied to the right and left common femoral artery respectively. At explantation three hours later, all BC-patches showed a thin gel like layer, most likely consisting of platelets, throughout the whole sur- face while the ePTFE-patch showed no, or minimal, signs of platelet adhesions. Conclusion: Theoretically the cellulose might be similar to autologous veins considering risk of infections and thrombo- genicity. The animal model and the graft material showed good potential in the pilot study. The survival study was discour- aging with the reason for occlusion still to be explained. Bacterial cellulose has a good potential but further development and studies need to be performed. [Arch Clin Exp Surg 2016; 5(2.000): 70-77]http://www.scopemed.org/fulltextpdf.php?mno=188676Bacterial celluloseanimal modelAV-fistulaarteriovenous fistulaacetobacter xylinum |
| spellingShingle | Johan Magnusson Katarina Bjorses Jan Holst Martin Malina Karolina Helczynska Biosynthetic bacterial cellulose graft as arteriovenous fistula and ndash; a complement to existing synthetic grafts? Archives of Clinical and Experimental Surgery Bacterial cellulose animal model AV-fistula arteriovenous fistula acetobacter xylinum |
| title | Biosynthetic bacterial cellulose graft as arteriovenous fistula and ndash; a complement to existing synthetic grafts? |
| title_full | Biosynthetic bacterial cellulose graft as arteriovenous fistula and ndash; a complement to existing synthetic grafts? |
| title_fullStr | Biosynthetic bacterial cellulose graft as arteriovenous fistula and ndash; a complement to existing synthetic grafts? |
| title_full_unstemmed | Biosynthetic bacterial cellulose graft as arteriovenous fistula and ndash; a complement to existing synthetic grafts? |
| title_short | Biosynthetic bacterial cellulose graft as arteriovenous fistula and ndash; a complement to existing synthetic grafts? |
| title_sort | biosynthetic bacterial cellulose graft as arteriovenous fistula and ndash a complement to existing synthetic grafts |
| topic | Bacterial cellulose animal model AV-fistula arteriovenous fistula acetobacter xylinum |
| url | http://www.scopemed.org/fulltextpdf.php?mno=188676 |
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