Distinctive Neuroanatomic Regions Involved in Cocaine-Induced Behavioral Sensitization in Mice
The present study aimed to characterize the phenomenon of behavioral sensitization to cocaine and to identify neuroanatomical structures involved in the induction and expression phases of this phenomenon. For this, in experiment 1 (induction phase), mice were treated with saline or cocaine every sec...
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MDPI AG
2023-01-01
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| Online Access: | https://www.mdpi.com/2227-9059/11/2/383 |
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| author | Renan dos Santos-Baldaia Raphael Wuo-Silva Viviam Sanabria Marilia A. Baldaia Thais S. Yokoyama Antonio Augusto Coppi André W. Hollais Eduardo A. V. Marinho Alexandre J. Oliveira-Lima Beatriz M. Longo |
| author_facet | Renan dos Santos-Baldaia Raphael Wuo-Silva Viviam Sanabria Marilia A. Baldaia Thais S. Yokoyama Antonio Augusto Coppi André W. Hollais Eduardo A. V. Marinho Alexandre J. Oliveira-Lima Beatriz M. Longo |
| author_sort | Renan dos Santos-Baldaia |
| collection | DOAJ |
| description | The present study aimed to characterize the phenomenon of behavioral sensitization to cocaine and to identify neuroanatomical structures involved in the induction and expression phases of this phenomenon. For this, in experiment 1 (induction phase), mice were treated with saline or cocaine every second day for 15 days (conditioning period), in the open-field or in their home-cages. In experiment 2 (expression phase), the same protocol was followed, except that after the conditioning period the animals were not manipulated for 10 days, and after this interval, animals were challenged with cocaine. Neuroanatomical structures involved in the induction and expression phases were identified by stereological quantification of c-Fos staining in the dorsomedial prefrontal cortex (dmPFC), nucleus accumbens core (NAc core and shell (NAc shell), basolateral amygdala (BLA), and ventral tegmental area (VTA). Neuroanatomical analysis indicated that in the induction phase, cocaine-conditioned animals had higher expression of c-Fos in the dmPFC, NAc core, BLA, and VTA, whereas in the expression phase, almost all areas had higher expression except for the VTA. Therefore, environmental context plays a major role in the induction and expression of behavioral sensitization, although not all structures that compose the mesolimbic system contribute to this phenomenon. |
| first_indexed | 2024-03-11T09:07:07Z |
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| id | doaj.art-8fd14d01e0e34db8a5d27b6984553b82 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-11T09:07:07Z |
| publishDate | 2023-01-01 |
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| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-8fd14d01e0e34db8a5d27b6984553b822023-11-16T19:17:26ZengMDPI AGBiomedicines2227-90592023-01-0111238310.3390/biomedicines11020383Distinctive Neuroanatomic Regions Involved in Cocaine-Induced Behavioral Sensitization in MiceRenan dos Santos-Baldaia0Raphael Wuo-Silva1Viviam Sanabria2Marilia A. Baldaia3Thais S. Yokoyama4Antonio Augusto Coppi5André W. Hollais6Eduardo A. V. Marinho7Alexandre J. Oliveira-Lima8Beatriz M. Longo9Laboratory of Neurophysiology, Department of Physiology, Universidade Federal de São Paulo, São Paulo CEP 04023-066, BrazilLaboratory of Neurophysiology, Department of Physiology, Universidade Federal de São Paulo, São Paulo CEP 04023-066, BrazilLaboratory of Neurophysiology, Department of Physiology, Universidade Federal de São Paulo, São Paulo CEP 04023-066, BrazilDepartment of Pharmacology, Universidade Federal de São Paulo, São Paulo CEP 04023-066, BrazilLaboratory of Neurophysiology, Department of Physiology, Universidade Federal de São Paulo, São Paulo CEP 04023-066, BrazilBristol Veterinary School, University of Bristol, Bristol BS40 5DU, UKLaboratory of Neurophysiology, Department of Physiology, Universidade Federal de São Paulo, São Paulo CEP 04023-066, BrazilLaboratory of Neurophysiology, Department of Physiology, Universidade Federal de São Paulo, São Paulo CEP 04023-066, BrazilLaboratory of Neurophysiology, Department of Physiology, Universidade Federal de São Paulo, São Paulo CEP 04023-066, BrazilLaboratory of Neurophysiology, Department of Physiology, Universidade Federal de São Paulo, São Paulo CEP 04023-066, BrazilThe present study aimed to characterize the phenomenon of behavioral sensitization to cocaine and to identify neuroanatomical structures involved in the induction and expression phases of this phenomenon. For this, in experiment 1 (induction phase), mice were treated with saline or cocaine every second day for 15 days (conditioning period), in the open-field or in their home-cages. In experiment 2 (expression phase), the same protocol was followed, except that after the conditioning period the animals were not manipulated for 10 days, and after this interval, animals were challenged with cocaine. Neuroanatomical structures involved in the induction and expression phases were identified by stereological quantification of c-Fos staining in the dorsomedial prefrontal cortex (dmPFC), nucleus accumbens core (NAc core and shell (NAc shell), basolateral amygdala (BLA), and ventral tegmental area (VTA). Neuroanatomical analysis indicated that in the induction phase, cocaine-conditioned animals had higher expression of c-Fos in the dmPFC, NAc core, BLA, and VTA, whereas in the expression phase, almost all areas had higher expression except for the VTA. Therefore, environmental context plays a major role in the induction and expression of behavioral sensitization, although not all structures that compose the mesolimbic system contribute to this phenomenon.https://www.mdpi.com/2227-9059/11/2/383cocainebehavioral sensitizationinduction phaseexpression phasebrain structuresc-Fos |
| spellingShingle | Renan dos Santos-Baldaia Raphael Wuo-Silva Viviam Sanabria Marilia A. Baldaia Thais S. Yokoyama Antonio Augusto Coppi André W. Hollais Eduardo A. V. Marinho Alexandre J. Oliveira-Lima Beatriz M. Longo Distinctive Neuroanatomic Regions Involved in Cocaine-Induced Behavioral Sensitization in Mice Biomedicines cocaine behavioral sensitization induction phase expression phase brain structures c-Fos |
| title | Distinctive Neuroanatomic Regions Involved in Cocaine-Induced Behavioral Sensitization in Mice |
| title_full | Distinctive Neuroanatomic Regions Involved in Cocaine-Induced Behavioral Sensitization in Mice |
| title_fullStr | Distinctive Neuroanatomic Regions Involved in Cocaine-Induced Behavioral Sensitization in Mice |
| title_full_unstemmed | Distinctive Neuroanatomic Regions Involved in Cocaine-Induced Behavioral Sensitization in Mice |
| title_short | Distinctive Neuroanatomic Regions Involved in Cocaine-Induced Behavioral Sensitization in Mice |
| title_sort | distinctive neuroanatomic regions involved in cocaine induced behavioral sensitization in mice |
| topic | cocaine behavioral sensitization induction phase expression phase brain structures c-Fos |
| url | https://www.mdpi.com/2227-9059/11/2/383 |
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