Expression of ZmLEA3, AOX2 and ZmPP2C genes in maize lines associated with tolerance to water deficit
ABSTRACT Knowing genes related to water deficit is a valuable tool to assist and accelerate the selection process of maize genotypes more adapted to drought conditions. Therefore, this work aimed to evaluate genes expression related to tolerance to water deficit in maize lines, using proteomic and t...
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| Format: | Article |
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Universidade Federal de Lavras
2019-12-01
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| Series: | Ciência e Agrotecnologia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542019000100233&tlng=en |
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| author | Thaís Lima Marques Renzo Garcia Von Pinho Édila Vilela de Resende Von Pinho Heloisa Oliveira dos Santos |
| author_facet | Thaís Lima Marques Renzo Garcia Von Pinho Édila Vilela de Resende Von Pinho Heloisa Oliveira dos Santos |
| author_sort | Thaís Lima Marques |
| collection | DOAJ |
| description | ABSTRACT Knowing genes related to water deficit is a valuable tool to assist and accelerate the selection process of maize genotypes more adapted to drought conditions. Therefore, this work aimed to evaluate genes expression related to tolerance to water deficit in maize lines, using proteomic and transcriptomic analyses. Two lines previously classified as tolerant (91-T) and non-tolerant (57-NT) to low water availability were used. Dried seeds, ears tips, and seedlings (collected at three, five, and seven days after sowing) of the two maize lines (91-T and 57-NT) were used in proteomic analyses. Seedlings were subject to two contrasting water-availability conditions (70% and 10% of the water retention capacity). The expression of catalase, peroxidase, and esterase enzymes and heat-resistant proteins were evaluated by electrophoresis. Dried seeds, ears tips, and seedlings (collected at seven days after sowing, subject to two contrasting water-availability conditions) of two maize lines (91-T and 57-NT) were used in the transcriptomic analyses. The expression of genes associated with tolerance to low water availability (ZmPP2C, ZmLEA3, and AOX2) was evaluated by the qRT-PCR technique. Results revealed that catalase, peroxidase, and esterase enzymes had a higher expression on the ears tips of the 91-T line. The expression of catalase and esterase enzymes reduced in seven-day-old seedlings in the two lines analyzed. Heat-resistant proteins had greater activity on the dried seeds of the 91-T line. The AOX2 gene was identified as a potential marker for the selection of maize genotypes tolerant to water deficit. |
| first_indexed | 2024-12-17T20:12:22Z |
| format | Article |
| id | doaj.art-8fd3f541876e499392acd7e9400e0069 |
| institution | Directory Open Access Journal |
| issn | 1981-1829 |
| language | English |
| last_indexed | 2024-12-17T20:12:22Z |
| publishDate | 2019-12-01 |
| publisher | Universidade Federal de Lavras |
| record_format | Article |
| series | Ciência e Agrotecnologia |
| spelling | doaj.art-8fd3f541876e499392acd7e9400e00692022-12-21T21:34:12ZengUniversidade Federal de LavrasCiência e Agrotecnologia1981-18292019-12-014310.1590/1413-7054201943022519Expression of ZmLEA3, AOX2 and ZmPP2C genes in maize lines associated with tolerance to water deficitThaís Lima Marqueshttps://orcid.org/0000-0001-7488-4919Renzo Garcia Von Pinhohttps://orcid.org/0000-0001-5276-2806Édila Vilela de Resende Von Pinhohttps://orcid.org/0000-0002-0757-0095Heloisa Oliveira dos Santoshttps://orcid.org/0000-0003-1384-4969ABSTRACT Knowing genes related to water deficit is a valuable tool to assist and accelerate the selection process of maize genotypes more adapted to drought conditions. Therefore, this work aimed to evaluate genes expression related to tolerance to water deficit in maize lines, using proteomic and transcriptomic analyses. Two lines previously classified as tolerant (91-T) and non-tolerant (57-NT) to low water availability were used. Dried seeds, ears tips, and seedlings (collected at three, five, and seven days after sowing) of the two maize lines (91-T and 57-NT) were used in proteomic analyses. Seedlings were subject to two contrasting water-availability conditions (70% and 10% of the water retention capacity). The expression of catalase, peroxidase, and esterase enzymes and heat-resistant proteins were evaluated by electrophoresis. Dried seeds, ears tips, and seedlings (collected at seven days after sowing, subject to two contrasting water-availability conditions) of two maize lines (91-T and 57-NT) were used in the transcriptomic analyses. The expression of genes associated with tolerance to low water availability (ZmPP2C, ZmLEA3, and AOX2) was evaluated by the qRT-PCR technique. Results revealed that catalase, peroxidase, and esterase enzymes had a higher expression on the ears tips of the 91-T line. The expression of catalase and esterase enzymes reduced in seven-day-old seedlings in the two lines analyzed. Heat-resistant proteins had greater activity on the dried seeds of the 91-T line. The AOX2 gene was identified as a potential marker for the selection of maize genotypes tolerant to water deficit.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542019000100233&tlng=enZea maysabiotic stresselectrophoresisqRT-PCR. |
| spellingShingle | Thaís Lima Marques Renzo Garcia Von Pinho Édila Vilela de Resende Von Pinho Heloisa Oliveira dos Santos Expression of ZmLEA3, AOX2 and ZmPP2C genes in maize lines associated with tolerance to water deficit Ciência e Agrotecnologia Zea mays abiotic stress electrophoresis qRT-PCR. |
| title | Expression of ZmLEA3, AOX2 and ZmPP2C genes in maize lines associated with tolerance to water deficit |
| title_full | Expression of ZmLEA3, AOX2 and ZmPP2C genes in maize lines associated with tolerance to water deficit |
| title_fullStr | Expression of ZmLEA3, AOX2 and ZmPP2C genes in maize lines associated with tolerance to water deficit |
| title_full_unstemmed | Expression of ZmLEA3, AOX2 and ZmPP2C genes in maize lines associated with tolerance to water deficit |
| title_short | Expression of ZmLEA3, AOX2 and ZmPP2C genes in maize lines associated with tolerance to water deficit |
| title_sort | expression of zmlea3 aox2 and zmpp2c genes in maize lines associated with tolerance to water deficit |
| topic | Zea mays abiotic stress electrophoresis qRT-PCR. |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542019000100233&tlng=en |
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