Development of a versatile nuclease prime editor with upgraded precision
Strategies to improve the specificity of nuclease-based prime editor (PEn) are needed. Here the authors report a 53BP1-inhibitory ubiquitin variant-assisted PEn platform (uPEn) to inhibit NHEJ and enable precise prime editing for generation of insertions, deletions and replacements.
Bibliographic Details
| Main Authors: |
Xiangyang Li,
Guiquan Zhang,
Shisheng Huang,
Yao Liu,
Jin Tang,
Mingtian Zhong,
Xin Wang,
Wenjun Sun,
Yuan Yao,
Quanjiang Ji,
Xiaolong Wang,
Jianghuai Liu,
Shiqiang Zhu,
Xingxu Huang |
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| Format: | Article
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| Language: | English |
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| Published: |
Nature Portfolio
2023-01-01
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| Series: | Nature Communications
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| Online Access: | https://doi.org/10.1038/s41467-023-35870-0
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