Bioconversion of Crude Glycerol into 1,3-Propanediol(1,3-PDO) with Bioelectrochemical System and Zero-Valent Iron Using <i>Klebsiella pneumoniae</i> L17

Crude glycerol is a major byproduct in the production of biodiesel and contains a large number of impurities. The transformation of crude glycerol into valuable compounds such as 1,3-propanediol (1,3-PDO) using clean and renewable processes, like bioconversion, is an important task for the future of...

Full description

Bibliographic Details
Main Authors: Da Seul Kong, Eun Joo Park, Sakuntala Mutyala, Minsoo Kim, Yunchul Cho, Sang Eun Oh, Changman Kim, Jung Rae Kim
Format: Article
Language:English
Published: MDPI AG 2021-10-01
Series:Energies
Subjects:
Online Access:https://www.mdpi.com/1996-1073/14/20/6806
_version_ 1797514666912514048
author Da Seul Kong
Eun Joo Park
Sakuntala Mutyala
Minsoo Kim
Yunchul Cho
Sang Eun Oh
Changman Kim
Jung Rae Kim
author_facet Da Seul Kong
Eun Joo Park
Sakuntala Mutyala
Minsoo Kim
Yunchul Cho
Sang Eun Oh
Changman Kim
Jung Rae Kim
author_sort Da Seul Kong
collection DOAJ
description Crude glycerol is a major byproduct in the production of biodiesel and contains a large number of impurities. The transformation of crude glycerol into valuable compounds such as 1,3-propanediol (1,3-PDO) using clean and renewable processes, like bioconversion, is an important task for the future of the chemical industry. In this study, 1,3-PDO bioproductions from crude and pure glycerol were estimated as 15.4 ± 0.8 and 11.4 ± 0.1 mmol/L, respectively. Because 1,3-PDO is a reductive metabolite that requires additional reducing energy, external supplements of electron for further improvement of 1,3-PDO biosynthesis were attempted using a bioelectrochemical system (BES) or zero-valent iron (ZVI). The conversions of crude and pure glycerol under electrode and iron-based cultivation were investigated for 1,3-PDO production accompanied by metabolic shift and cell growth. The BES-based conversion produced 32.6 ± 0.6 mmol/L of 1,3-PDO with ZVI implementation.
first_indexed 2024-03-10T06:34:50Z
format Article
id doaj.art-8fdf48a71297437e9fda39cfe9d51640
institution Directory Open Access Journal
issn 1996-1073
language English
last_indexed 2024-03-10T06:34:50Z
publishDate 2021-10-01
publisher MDPI AG
record_format Article
series Energies
spelling doaj.art-8fdf48a71297437e9fda39cfe9d516402023-11-22T18:09:08ZengMDPI AGEnergies1996-10732021-10-011420680610.3390/en14206806Bioconversion of Crude Glycerol into 1,3-Propanediol(1,3-PDO) with Bioelectrochemical System and Zero-Valent Iron Using <i>Klebsiella pneumoniae</i> L17Da Seul Kong0Eun Joo Park1Sakuntala Mutyala2Minsoo Kim3Yunchul Cho4Sang Eun Oh5Changman Kim6Jung Rae Kim7School of Chemical Engineering, Pusan National University, Busan 46241, KoreaSchool of Chemical Engineering, Pusan National University, Busan 46241, KoreaSchool of Chemical Engineering, Pusan National University, Busan 46241, KoreaSchool of Chemical Engineering, Pusan National University, Busan 46241, KoreaDepartment of Environmental Engineering, Daejeon University, 62 Daehak-ro, Dong-gu, Daejeon 34520, KoreaDepartment of Biological Environment, Kangwon National University, Gangwondo, Chuncheon 200-701, KoreaAdvanced Biofuel and Bioproducts Process Development Unit, Lawrence Berkeley National Laboratory, Emeryville, CA 94608, USASchool of Chemical Engineering, Pusan National University, Busan 46241, KoreaCrude glycerol is a major byproduct in the production of biodiesel and contains a large number of impurities. The transformation of crude glycerol into valuable compounds such as 1,3-propanediol (1,3-PDO) using clean and renewable processes, like bioconversion, is an important task for the future of the chemical industry. In this study, 1,3-PDO bioproductions from crude and pure glycerol were estimated as 15.4 ± 0.8 and 11.4 ± 0.1 mmol/L, respectively. Because 1,3-PDO is a reductive metabolite that requires additional reducing energy, external supplements of electron for further improvement of 1,3-PDO biosynthesis were attempted using a bioelectrochemical system (BES) or zero-valent iron (ZVI). The conversions of crude and pure glycerol under electrode and iron-based cultivation were investigated for 1,3-PDO production accompanied by metabolic shift and cell growth. The BES-based conversion produced 32.6 ± 0.6 mmol/L of 1,3-PDO with ZVI implementation.https://www.mdpi.com/1996-1073/14/20/6806crude glycerol<i>Klebsiella pneumoniae</i> L171,3-propanediol (1,3-PDO)bioelectrochemical system (BES)zero-valent iron (ZVI)
spellingShingle Da Seul Kong
Eun Joo Park
Sakuntala Mutyala
Minsoo Kim
Yunchul Cho
Sang Eun Oh
Changman Kim
Jung Rae Kim
Bioconversion of Crude Glycerol into 1,3-Propanediol(1,3-PDO) with Bioelectrochemical System and Zero-Valent Iron Using <i>Klebsiella pneumoniae</i> L17
Energies
crude glycerol
<i>Klebsiella pneumoniae</i> L17
1,3-propanediol (1,3-PDO)
bioelectrochemical system (BES)
zero-valent iron (ZVI)
title Bioconversion of Crude Glycerol into 1,3-Propanediol(1,3-PDO) with Bioelectrochemical System and Zero-Valent Iron Using <i>Klebsiella pneumoniae</i> L17
title_full Bioconversion of Crude Glycerol into 1,3-Propanediol(1,3-PDO) with Bioelectrochemical System and Zero-Valent Iron Using <i>Klebsiella pneumoniae</i> L17
title_fullStr Bioconversion of Crude Glycerol into 1,3-Propanediol(1,3-PDO) with Bioelectrochemical System and Zero-Valent Iron Using <i>Klebsiella pneumoniae</i> L17
title_full_unstemmed Bioconversion of Crude Glycerol into 1,3-Propanediol(1,3-PDO) with Bioelectrochemical System and Zero-Valent Iron Using <i>Klebsiella pneumoniae</i> L17
title_short Bioconversion of Crude Glycerol into 1,3-Propanediol(1,3-PDO) with Bioelectrochemical System and Zero-Valent Iron Using <i>Klebsiella pneumoniae</i> L17
title_sort bioconversion of crude glycerol into 1 3 propanediol 1 3 pdo with bioelectrochemical system and zero valent iron using i klebsiella pneumoniae i l17
topic crude glycerol
<i>Klebsiella pneumoniae</i> L17
1,3-propanediol (1,3-PDO)
bioelectrochemical system (BES)
zero-valent iron (ZVI)
url https://www.mdpi.com/1996-1073/14/20/6806
work_keys_str_mv AT daseulkong bioconversionofcrudeglycerolinto13propanediol13pdowithbioelectrochemicalsystemandzerovalentironusingiklebsiellapneumoniaeil17
AT eunjoopark bioconversionofcrudeglycerolinto13propanediol13pdowithbioelectrochemicalsystemandzerovalentironusingiklebsiellapneumoniaeil17
AT sakuntalamutyala bioconversionofcrudeglycerolinto13propanediol13pdowithbioelectrochemicalsystemandzerovalentironusingiklebsiellapneumoniaeil17
AT minsookim bioconversionofcrudeglycerolinto13propanediol13pdowithbioelectrochemicalsystemandzerovalentironusingiklebsiellapneumoniaeil17
AT yunchulcho bioconversionofcrudeglycerolinto13propanediol13pdowithbioelectrochemicalsystemandzerovalentironusingiklebsiellapneumoniaeil17
AT sangeunoh bioconversionofcrudeglycerolinto13propanediol13pdowithbioelectrochemicalsystemandzerovalentironusingiklebsiellapneumoniaeil17
AT changmankim bioconversionofcrudeglycerolinto13propanediol13pdowithbioelectrochemicalsystemandzerovalentironusingiklebsiellapneumoniaeil17
AT jungraekim bioconversionofcrudeglycerolinto13propanediol13pdowithbioelectrochemicalsystemandzerovalentironusingiklebsiellapneumoniaeil17