Niche-specific gene expression in a parasitic nematode; increased expression of immunomodulators in Teladorsagia circumcincta larvae derived from host mucosa
Abstract Metazoan parasites have to survive in many different niches in order to complete their life-cycles. In the absence of reliable methods to manipulate parasite genomes and/or proteomes, identification of the molecules critical for parasite survival within these niches has largely depended on...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-07092-0 |
| _version_ | 1818840708860084224 |
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| author | Tom N. McNeilly David Frew Stewart T. G. Burgess Harry Wright David J. Bartley Yvonne Bartley Alasdair J. Nisbet |
| author_facet | Tom N. McNeilly David Frew Stewart T. G. Burgess Harry Wright David J. Bartley Yvonne Bartley Alasdair J. Nisbet |
| author_sort | Tom N. McNeilly |
| collection | DOAJ |
| description | Abstract Metazoan parasites have to survive in many different niches in order to complete their life-cycles. In the absence of reliable methods to manipulate parasite genomes and/or proteomes, identification of the molecules critical for parasite survival within these niches has largely depended on comparative transcriptomic and proteomic analyses of different developmental stages of the parasite; however, changes may reflect differences associated with transition between developmental stages rather than specific adaptations to a particular niche. In this study, we compared the transcriptome of two fourth-stage larval populations of the nematode parasite, Teladorsagia circumcincta, which were of the same developmental stage but differed in their location within the abomasum, being either mucosal-dwelling (MD) or lumen-dwelling (LD). Using RNAseq, we identified 57 transcripts which were significantly differentially expressed between MD and LD larvae. Of these transcripts, the majority (54/57) were up-regulated in MD larvae, one of which encoded for an ShKT-domain containing protein, Tck6, capable of modulating ovine T cell cytokine responses. Other differentially expressed transcripts included homologues of ASP-like proteins, proteases, or excretory-secretory proteins of unknown function. Our study demonstrates the utility of niche- rather than stage-specific analysis of parasite transcriptomes to identify parasite molecules of potential importance for survival within the host. |
| first_indexed | 2024-12-19T04:14:29Z |
| format | Article |
| id | doaj.art-8fe00dbb773a47bfa5bcdfa8f679afdf |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T04:14:29Z |
| publishDate | 2017-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-8fe00dbb773a47bfa5bcdfa8f679afdf2022-12-21T20:36:20ZengNature PortfolioScientific Reports2045-23222017-08-017111210.1038/s41598-017-07092-0Niche-specific gene expression in a parasitic nematode; increased expression of immunomodulators in Teladorsagia circumcincta larvae derived from host mucosaTom N. McNeilly0David Frew1Stewart T. G. Burgess2Harry Wright3David J. Bartley4Yvonne Bartley5Alasdair J. Nisbet6Moredun Research InstituteMoredun Research InstituteMoredun Research InstituteMoredun Research InstituteMoredun Research InstituteMoredun Research InstituteMoredun Research InstituteAbstract Metazoan parasites have to survive in many different niches in order to complete their life-cycles. In the absence of reliable methods to manipulate parasite genomes and/or proteomes, identification of the molecules critical for parasite survival within these niches has largely depended on comparative transcriptomic and proteomic analyses of different developmental stages of the parasite; however, changes may reflect differences associated with transition between developmental stages rather than specific adaptations to a particular niche. In this study, we compared the transcriptome of two fourth-stage larval populations of the nematode parasite, Teladorsagia circumcincta, which were of the same developmental stage but differed in their location within the abomasum, being either mucosal-dwelling (MD) or lumen-dwelling (LD). Using RNAseq, we identified 57 transcripts which were significantly differentially expressed between MD and LD larvae. Of these transcripts, the majority (54/57) were up-regulated in MD larvae, one of which encoded for an ShKT-domain containing protein, Tck6, capable of modulating ovine T cell cytokine responses. Other differentially expressed transcripts included homologues of ASP-like proteins, proteases, or excretory-secretory proteins of unknown function. Our study demonstrates the utility of niche- rather than stage-specific analysis of parasite transcriptomes to identify parasite molecules of potential importance for survival within the host.https://doi.org/10.1038/s41598-017-07092-0 |
| spellingShingle | Tom N. McNeilly David Frew Stewart T. G. Burgess Harry Wright David J. Bartley Yvonne Bartley Alasdair J. Nisbet Niche-specific gene expression in a parasitic nematode; increased expression of immunomodulators in Teladorsagia circumcincta larvae derived from host mucosa Scientific Reports |
| title | Niche-specific gene expression in a parasitic nematode; increased expression of immunomodulators in Teladorsagia circumcincta larvae derived from host mucosa |
| title_full | Niche-specific gene expression in a parasitic nematode; increased expression of immunomodulators in Teladorsagia circumcincta larvae derived from host mucosa |
| title_fullStr | Niche-specific gene expression in a parasitic nematode; increased expression of immunomodulators in Teladorsagia circumcincta larvae derived from host mucosa |
| title_full_unstemmed | Niche-specific gene expression in a parasitic nematode; increased expression of immunomodulators in Teladorsagia circumcincta larvae derived from host mucosa |
| title_short | Niche-specific gene expression in a parasitic nematode; increased expression of immunomodulators in Teladorsagia circumcincta larvae derived from host mucosa |
| title_sort | niche specific gene expression in a parasitic nematode increased expression of immunomodulators in teladorsagia circumcincta larvae derived from host mucosa |
| url | https://doi.org/10.1038/s41598-017-07092-0 |
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