Obtaining of transgenic potato (Solanum tuberosum L.) cultivar IPB CP3 containing LYZ‐C gene resistant to bacterial wilt disease
Bacterial wilt caused by Ralstonia solanacearum is one of the most important bacterial diseases in potato production. This study aimed to obtain the transgenic potato (Solanum tuberosum L.) cultivar IPB CP3, containing LYZ‐C gene encoding for lysozyme type C, resistant to bacterial disease caused by...
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada, Yogyakarta
2021-03-01
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| Series: | Indonesian Journal of Biotechnology |
| Subjects: | |
| Online Access: | https://jurnal.ugm.ac.id/ijbiotech/article/view/61682 |
| _version_ | 1811171011369893888 |
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| author | Pasmawati Pasmawati Aris Tjahjoleksono Suharsono Suharsono |
| author_facet | Pasmawati Pasmawati Aris Tjahjoleksono Suharsono Suharsono |
| author_sort | Pasmawati Pasmawati |
| collection | DOAJ |
| description | Bacterial wilt caused by Ralstonia solanacearum is one of the most important bacterial diseases in potato production. This study aimed to obtain the transgenic potato (Solanum tuberosum L.) cultivar IPB CP3, containing LYZ‐C gene encoding for lysozyme type C, resistant to bacterial disease caused by R. solanacearum. Genetic transformation using Agrobacterium tumefaciens LBA4404 to 124 internode explants resulted in the transformation efficiency of about 47.58% with a regeneration efficiency of approximately 30.51%. Gene integration analysis showed that 16 clones were confirmed as transgenic clones containing the LYZ‐C gene. Analysis of resistance to R. solanacearum of three transgenic clones showed that all three transgenic clones were more resistant than a non‐transgenic one. This result showed that the LYZ‐C gene integrated in the genome of transgenic potato increased the resistance of potato plants to R. solanacearum. We obtained two transgenic clones considered resistant to bacterial wilt disease. |
| first_indexed | 2024-04-10T17:06:23Z |
| format | Article |
| id | doaj.art-8fe3a4de27a24f10a0c4dfe43c5fc048 |
| institution | Directory Open Access Journal |
| issn | 0853-8654 2089-2241 |
| language | English |
| last_indexed | 2024-04-10T17:06:23Z |
| publishDate | 2021-03-01 |
| publisher | Universitas Gadjah Mada, Yogyakarta |
| record_format | Article |
| series | Indonesian Journal of Biotechnology |
| spelling | doaj.art-8fe3a4de27a24f10a0c4dfe43c5fc0482023-02-06T03:00:10ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412021-03-01261485310.22146/ijbiotech.6168229527Obtaining of transgenic potato (Solanum tuberosum L.) cultivar IPB CP3 containing LYZ‐C gene resistant to bacterial wilt diseasePasmawati Pasmawati0Aris Tjahjoleksono1Suharsono Suharsono2Graduate School of Biotechnology, IPB University, Bogor 16680; Research Center for Bioresources and Biotechnology, IPB University, Bogor 16680Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680Graduate School of Biotechnology, IPB University, Bogor 16680; Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680; Research Center for Bioresources and Biotechnology, IPB University, Bogor 16680Bacterial wilt caused by Ralstonia solanacearum is one of the most important bacterial diseases in potato production. This study aimed to obtain the transgenic potato (Solanum tuberosum L.) cultivar IPB CP3, containing LYZ‐C gene encoding for lysozyme type C, resistant to bacterial disease caused by R. solanacearum. Genetic transformation using Agrobacterium tumefaciens LBA4404 to 124 internode explants resulted in the transformation efficiency of about 47.58% with a regeneration efficiency of approximately 30.51%. Gene integration analysis showed that 16 clones were confirmed as transgenic clones containing the LYZ‐C gene. Analysis of resistance to R. solanacearum of three transgenic clones showed that all three transgenic clones were more resistant than a non‐transgenic one. This result showed that the LYZ‐C gene integrated in the genome of transgenic potato increased the resistance of potato plants to R. solanacearum. We obtained two transgenic clones considered resistant to bacterial wilt disease.https://jurnal.ugm.ac.id/ijbiotech/article/view/61682bacterial wiltgenetic transformationlysozymepotatotransgenic |
| spellingShingle | Pasmawati Pasmawati Aris Tjahjoleksono Suharsono Suharsono Obtaining of transgenic potato (Solanum tuberosum L.) cultivar IPB CP3 containing LYZ‐C gene resistant to bacterial wilt disease Indonesian Journal of Biotechnology bacterial wilt genetic transformation lysozyme potato transgenic |
| title | Obtaining of transgenic potato (Solanum tuberosum L.) cultivar IPB CP3 containing LYZ‐C gene resistant to bacterial wilt disease |
| title_full | Obtaining of transgenic potato (Solanum tuberosum L.) cultivar IPB CP3 containing LYZ‐C gene resistant to bacterial wilt disease |
| title_fullStr | Obtaining of transgenic potato (Solanum tuberosum L.) cultivar IPB CP3 containing LYZ‐C gene resistant to bacterial wilt disease |
| title_full_unstemmed | Obtaining of transgenic potato (Solanum tuberosum L.) cultivar IPB CP3 containing LYZ‐C gene resistant to bacterial wilt disease |
| title_short | Obtaining of transgenic potato (Solanum tuberosum L.) cultivar IPB CP3 containing LYZ‐C gene resistant to bacterial wilt disease |
| title_sort | obtaining of transgenic potato solanum tuberosum l cultivar ipb cp3 containing lyz c gene resistant to bacterial wilt disease |
| topic | bacterial wilt genetic transformation lysozyme potato transgenic |
| url | https://jurnal.ugm.ac.id/ijbiotech/article/view/61682 |
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