Graphene-Assisted Sensor for Rapid Detection of Antibiotic Resistance in Escherichia coli
In recent years, antibiotic-resistant bacteria caused by antibiotic abuse in the medical industry have become a new environmental pollutant that endangers public health. Therefore, it is necessary to establish a detection method for evaluating drug-resistant bacteria. In this work, we used Escherich...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-05-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2021.696906/full |
| _version_ | 1818599776402276352 |
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| author | Chunlei Li Feng Sun |
| author_facet | Chunlei Li Feng Sun |
| author_sort | Chunlei Li |
| collection | DOAJ |
| description | In recent years, antibiotic-resistant bacteria caused by antibiotic abuse in the medical industry have become a new environmental pollutant that endangers public health. Therefore, it is necessary to establish a detection method for evaluating drug-resistant bacteria. In this work, we used Escherichia coli as a target model and proposed a method to evaluate its drug resistance for three antibiotics. Graphene dispersion was used to co-mix with E. coli cells for the purpose of increasing the current signal. This electrochemical-based sensor allows the evaluation of the activity of E. coli on the electrode surface. When antibiotics were present, the electrocatalytic reduction signal was diminished because of the reduced activity of E. coli. Based on the difference in the electrochemical reduction signal, we can evaluate the antibiotic resistance of different E. coli strains. |
| first_indexed | 2024-12-16T12:24:58Z |
| format | Article |
| id | doaj.art-8fe40712915c4c8e8f3969a91c6a52e4 |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-12-16T12:24:58Z |
| publishDate | 2021-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-8fe40712915c4c8e8f3969a91c6a52e42022-12-21T22:31:51ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462021-05-01910.3389/fchem.2021.696906696906Graphene-Assisted Sensor for Rapid Detection of Antibiotic Resistance in Escherichia coliChunlei Li0Feng Sun1Department of Gastroenterology, Jiaozhou Central Hospital, Jiaozhou, ChinaDepartment of Colorectal and Anal Surgery, The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou, ChinaIn recent years, antibiotic-resistant bacteria caused by antibiotic abuse in the medical industry have become a new environmental pollutant that endangers public health. Therefore, it is necessary to establish a detection method for evaluating drug-resistant bacteria. In this work, we used Escherichia coli as a target model and proposed a method to evaluate its drug resistance for three antibiotics. Graphene dispersion was used to co-mix with E. coli cells for the purpose of increasing the current signal. This electrochemical-based sensor allows the evaluation of the activity of E. coli on the electrode surface. When antibiotics were present, the electrocatalytic reduction signal was diminished because of the reduced activity of E. coli. Based on the difference in the electrochemical reduction signal, we can evaluate the antibiotic resistance of different E. coli strains.https://www.frontiersin.org/articles/10.3389/fchem.2021.696906/fullelectrochemical sensorsdrug-resistant strainsactivity determinationantibioticselectrode modificationcatalytic reduction current |
| spellingShingle | Chunlei Li Feng Sun Graphene-Assisted Sensor for Rapid Detection of Antibiotic Resistance in Escherichia coli Frontiers in Chemistry electrochemical sensors drug-resistant strains activity determination antibiotics electrode modification catalytic reduction current |
| title | Graphene-Assisted Sensor for Rapid Detection of Antibiotic Resistance in Escherichia coli |
| title_full | Graphene-Assisted Sensor for Rapid Detection of Antibiotic Resistance in Escherichia coli |
| title_fullStr | Graphene-Assisted Sensor for Rapid Detection of Antibiotic Resistance in Escherichia coli |
| title_full_unstemmed | Graphene-Assisted Sensor for Rapid Detection of Antibiotic Resistance in Escherichia coli |
| title_short | Graphene-Assisted Sensor for Rapid Detection of Antibiotic Resistance in Escherichia coli |
| title_sort | graphene assisted sensor for rapid detection of antibiotic resistance in escherichia coli |
| topic | electrochemical sensors drug-resistant strains activity determination antibiotics electrode modification catalytic reduction current |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2021.696906/full |
| work_keys_str_mv | AT chunleili grapheneassistedsensorforrapiddetectionofantibioticresistanceinescherichiacoli AT fengsun grapheneassistedsensorforrapiddetectionofantibioticresistanceinescherichiacoli |