Early-phase rotator training impairs tissue repair and functional recovery after spinal cord injury
Spinal cord injury (SCI) is a devastating disorder that often results in severe sensorimotor function impairment with limited recovery of function. In recent years, rehabilitation training for spinal cord injury has gradually emerged, and some of them play an important role in the repair of spinal c...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-07-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023053665 |
| _version_ | 1797771441697980416 |
|---|---|
| author | Junhao Deng Fanqi Meng Jianpeng Gao Kexue Zhang Zhongyang Liu Ming Li Xiao Liu Jiantao Li Yu Wang Licheng Zhang Peifu Tang |
| author_facet | Junhao Deng Fanqi Meng Jianpeng Gao Kexue Zhang Zhongyang Liu Ming Li Xiao Liu Jiantao Li Yu Wang Licheng Zhang Peifu Tang |
| author_sort | Junhao Deng |
| collection | DOAJ |
| description | Spinal cord injury (SCI) is a devastating disorder that often results in severe sensorimotor function impairment with limited recovery of function. In recent years, rehabilitation training for spinal cord injury has gradually emerged, and some of them play an important role in the repair of spinal cord injury However, the optimal training regimen for SCI remains to be determined. In this study, we explore the effects of rotarod training (began at 7 days post-injury) on the recovery of motor function after SCI, as well as its possible repair mechanism from the aspects of function and histopathological changes, the behaviors of specific trophic factors and cytokines, and the expression profile of specific genes. Multiple functional assessments showed that rotarod training initiated at 7 days post-injury is unsuitable for promoting neuro-electrophysiological improvement and trunk stability, but impaired functional coordination and motor recovery. In addition, rotarod training has negative effects on spinal cord repair after SCI, which is manifested as an increase of lesion area, a decrease in neuronal viability, a deterioration in immuno-microenvironment and remyelination, a significant reduction in the expression of trophic factors and an increase in the expression of pro-inflammatory factors. RNA sequencing suggested that the genes associated with angiogenesis and synaptogenesis were significantly downregulated and the PI3K-AKT pathway was inhibited, which was detrimental to spinal cord repair and impeded nerve regeneration. These results indicate that immediate rotarod training after SCI is currently unsuitable for rehabilitation in mice. |
| first_indexed | 2024-03-12T21:37:37Z |
| format | Article |
| id | doaj.art-8fe61a315f9240ac96700ba53ceee5cf |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-12T21:37:37Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-8fe61a315f9240ac96700ba53ceee5cf2023-07-27T05:58:44ZengElsevierHeliyon2405-84402023-07-0197e18158Early-phase rotator training impairs tissue repair and functional recovery after spinal cord injuryJunhao Deng0Fanqi Meng1Jianpeng Gao2Kexue Zhang3Zhongyang Liu4Ming Li5Xiao Liu6Jiantao Li7Yu Wang8Licheng Zhang9Peifu Tang10Department of Orthopedics, The Chinese PLA General Hospital, Beijing, 100853, China; School of Life Sciences, Tsinghua University, Beijing, 100084, ChinaDepartment of Anesthesiology, Xuanwu Hospital Capital Medical University, Beijing, 100050, ChinaDepartment of Orthopedics, The Chinese PLA General Hospital, Beijing, 100853, ChinaDepartment of Pediatric Surgery, The Chinese PLA General Hospital, Beijing, 100853, ChinaDepartment of Orthopedics, The Chinese PLA General Hospital, Beijing, 100853, ChinaDepartment of Orthopedics, The Chinese PLA General Hospital, Beijing, 100853, ChinaDepartment of Orthopedics, The Chinese PLA General Hospital, Beijing, 100853, ChinaDepartment of Orthopedics, The Chinese PLA General Hospital, Beijing, 100853, ChinaBeijing Key Lab of Regenerative Medicine in Orthopaedics, Key Laboratory of Musculoskeletal Trauma and War Injuries PLA, Institue of Orthopaedics, The Chinese PLA General Hospital, Beijing, 100853, ChinaDepartment of Orthopedics, The Chinese PLA General Hospital, Beijing, 100853, China; School of Life Sciences, Tsinghua University, Beijing, 100084, China; Corresponding author. Department of Orthopedics, The Chinese PLA General Hospital, Beijing, 100853, ChinaDepartment of Orthopedics, The Chinese PLA General Hospital, Beijing, 100853, China; School of Life Sciences, Tsinghua University, Beijing, 100084, ChinaSpinal cord injury (SCI) is a devastating disorder that often results in severe sensorimotor function impairment with limited recovery of function. In recent years, rehabilitation training for spinal cord injury has gradually emerged, and some of them play an important role in the repair of spinal cord injury However, the optimal training regimen for SCI remains to be determined. In this study, we explore the effects of rotarod training (began at 7 days post-injury) on the recovery of motor function after SCI, as well as its possible repair mechanism from the aspects of function and histopathological changes, the behaviors of specific trophic factors and cytokines, and the expression profile of specific genes. Multiple functional assessments showed that rotarod training initiated at 7 days post-injury is unsuitable for promoting neuro-electrophysiological improvement and trunk stability, but impaired functional coordination and motor recovery. In addition, rotarod training has negative effects on spinal cord repair after SCI, which is manifested as an increase of lesion area, a decrease in neuronal viability, a deterioration in immuno-microenvironment and remyelination, a significant reduction in the expression of trophic factors and an increase in the expression of pro-inflammatory factors. RNA sequencing suggested that the genes associated with angiogenesis and synaptogenesis were significantly downregulated and the PI3K-AKT pathway was inhibited, which was detrimental to spinal cord repair and impeded nerve regeneration. These results indicate that immediate rotarod training after SCI is currently unsuitable for rehabilitation in mice.http://www.sciencedirect.com/science/article/pii/S2405844023053665Spinal cord injuryRotarod trainingRehabilitative trainingInflammationEarly-phase training |
| spellingShingle | Junhao Deng Fanqi Meng Jianpeng Gao Kexue Zhang Zhongyang Liu Ming Li Xiao Liu Jiantao Li Yu Wang Licheng Zhang Peifu Tang Early-phase rotator training impairs tissue repair and functional recovery after spinal cord injury Heliyon Spinal cord injury Rotarod training Rehabilitative training Inflammation Early-phase training |
| title | Early-phase rotator training impairs tissue repair and functional recovery after spinal cord injury |
| title_full | Early-phase rotator training impairs tissue repair and functional recovery after spinal cord injury |
| title_fullStr | Early-phase rotator training impairs tissue repair and functional recovery after spinal cord injury |
| title_full_unstemmed | Early-phase rotator training impairs tissue repair and functional recovery after spinal cord injury |
| title_short | Early-phase rotator training impairs tissue repair and functional recovery after spinal cord injury |
| title_sort | early phase rotator training impairs tissue repair and functional recovery after spinal cord injury |
| topic | Spinal cord injury Rotarod training Rehabilitative training Inflammation Early-phase training |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023053665 |
| work_keys_str_mv | AT junhaodeng earlyphaserotatortrainingimpairstissuerepairandfunctionalrecoveryafterspinalcordinjury AT fanqimeng earlyphaserotatortrainingimpairstissuerepairandfunctionalrecoveryafterspinalcordinjury AT jianpenggao earlyphaserotatortrainingimpairstissuerepairandfunctionalrecoveryafterspinalcordinjury AT kexuezhang earlyphaserotatortrainingimpairstissuerepairandfunctionalrecoveryafterspinalcordinjury AT zhongyangliu earlyphaserotatortrainingimpairstissuerepairandfunctionalrecoveryafterspinalcordinjury AT mingli earlyphaserotatortrainingimpairstissuerepairandfunctionalrecoveryafterspinalcordinjury AT xiaoliu earlyphaserotatortrainingimpairstissuerepairandfunctionalrecoveryafterspinalcordinjury AT jiantaoli earlyphaserotatortrainingimpairstissuerepairandfunctionalrecoveryafterspinalcordinjury AT yuwang earlyphaserotatortrainingimpairstissuerepairandfunctionalrecoveryafterspinalcordinjury AT lichengzhang earlyphaserotatortrainingimpairstissuerepairandfunctionalrecoveryafterspinalcordinjury AT peifutang earlyphaserotatortrainingimpairstissuerepairandfunctionalrecoveryafterspinalcordinjury |