Analisis Kualitatif dan Kuantitatif Metabolit Sekunder Ekstrak Biji Kelor (Moringa oleifera Lam.) dengan Metode Spektrofotometri UV-Vis
This study aims to determine the content and total levels of secondary metabolites of ethanol extract of moringa seed (Moringa oleifera Lam.). Extraction of moringa seed using the maceration method to obtain a filtrate. The filtrate obtained was concentrated using a rotary evaporator to obtain a con...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Tadulako University
2020-12-01
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| Series: | Kovalen: Jurnal Riset Kimia |
| Online Access: | https://bestjournal.untad.ac.id/index.php/kovalen/article/view/15324 |
| _version_ | 1797792378100121600 |
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| author | Tien Wahyu Handayani Yulistien Yusuf Joni Tandi |
| author_facet | Tien Wahyu Handayani Yulistien Yusuf Joni Tandi |
| author_sort | Tien Wahyu Handayani |
| collection | DOAJ |
| description | This study aims to determine the content and total levels of secondary metabolites of ethanol extract of moringa seed (Moringa oleifera Lam.). Extraction of moringa seed using the maceration method to obtain a filtrate. The filtrate obtained was concentrated using a rotary evaporator to obtain a concentrated extract. The extract was tested qualitatively for alkaloids, flavonoids, saponins, and tannins using a suitable reagent with the test parameters. The quantitative test was using UV-Visible spectrophotometry. Alkaloids using test parameters equivalent total alkaloid quinine, flavonoids use parameter test equivalent of the total flavonoids quercetin, saponin using test parameters from Quillaja total saponins and tannins quantitative bark using test parameters total tannin tannic acid equivalent. The result showed that moringa seed positive for alkaloids characterized by orange deposition, flavonoids are characterized by the formation of the yellow color orange, saponin their stable foam, and tannins are marked in black. Quantitative test results alkaloids of 916,87 µg/g, flavonoids of 0.255%, saponin of 6.367%, and tannin of 3,724.5 µg/g.
Keywords: Moringa oleifera Lam., secondary metabolites, spectrophotometry |
| first_indexed | 2024-03-13T02:32:19Z |
| format | Article |
| id | doaj.art-8fe93d51e7184996ac42892025eacaba |
| institution | Directory Open Access Journal |
| issn | 2477-5398 |
| language | English |
| last_indexed | 2024-03-13T02:32:19Z |
| publishDate | 2020-12-01 |
| publisher | Tadulako University |
| record_format | Article |
| series | Kovalen: Jurnal Riset Kimia |
| spelling | doaj.art-8fe93d51e7184996ac42892025eacaba2023-06-29T13:04:27ZengTadulako UniversityKovalen: Jurnal Riset Kimia2477-53982020-12-016323023810.22487/kovalen.2020.v6.i3.1532415324Analisis Kualitatif dan Kuantitatif Metabolit Sekunder Ekstrak Biji Kelor (Moringa oleifera Lam.) dengan Metode Spektrofotometri UV-VisTien Wahyu Handayani0Yulistien Yusuf1Joni Tandi2Program Studi S1 Farmasi, STIFA Pelita Mas PaluProgram Studi S1 Farmasi, STIFA Pelita Mas PaluProgram Studi S1 Farmasi, STIFA Pelita Mas PaluThis study aims to determine the content and total levels of secondary metabolites of ethanol extract of moringa seed (Moringa oleifera Lam.). Extraction of moringa seed using the maceration method to obtain a filtrate. The filtrate obtained was concentrated using a rotary evaporator to obtain a concentrated extract. The extract was tested qualitatively for alkaloids, flavonoids, saponins, and tannins using a suitable reagent with the test parameters. The quantitative test was using UV-Visible spectrophotometry. Alkaloids using test parameters equivalent total alkaloid quinine, flavonoids use parameter test equivalent of the total flavonoids quercetin, saponin using test parameters from Quillaja total saponins and tannins quantitative bark using test parameters total tannin tannic acid equivalent. The result showed that moringa seed positive for alkaloids characterized by orange deposition, flavonoids are characterized by the formation of the yellow color orange, saponin their stable foam, and tannins are marked in black. Quantitative test results alkaloids of 916,87 µg/g, flavonoids of 0.255%, saponin of 6.367%, and tannin of 3,724.5 µg/g. Keywords: Moringa oleifera Lam., secondary metabolites, spectrophotometryhttps://bestjournal.untad.ac.id/index.php/kovalen/article/view/15324 |
| spellingShingle | Tien Wahyu Handayani Yulistien Yusuf Joni Tandi Analisis Kualitatif dan Kuantitatif Metabolit Sekunder Ekstrak Biji Kelor (Moringa oleifera Lam.) dengan Metode Spektrofotometri UV-Vis Kovalen: Jurnal Riset Kimia |
| title | Analisis Kualitatif dan Kuantitatif Metabolit Sekunder Ekstrak Biji Kelor (Moringa oleifera Lam.) dengan Metode Spektrofotometri UV-Vis |
| title_full | Analisis Kualitatif dan Kuantitatif Metabolit Sekunder Ekstrak Biji Kelor (Moringa oleifera Lam.) dengan Metode Spektrofotometri UV-Vis |
| title_fullStr | Analisis Kualitatif dan Kuantitatif Metabolit Sekunder Ekstrak Biji Kelor (Moringa oleifera Lam.) dengan Metode Spektrofotometri UV-Vis |
| title_full_unstemmed | Analisis Kualitatif dan Kuantitatif Metabolit Sekunder Ekstrak Biji Kelor (Moringa oleifera Lam.) dengan Metode Spektrofotometri UV-Vis |
| title_short | Analisis Kualitatif dan Kuantitatif Metabolit Sekunder Ekstrak Biji Kelor (Moringa oleifera Lam.) dengan Metode Spektrofotometri UV-Vis |
| title_sort | analisis kualitatif dan kuantitatif metabolit sekunder ekstrak biji kelor moringa oleifera lam dengan metode spektrofotometri uv vis |
| url | https://bestjournal.untad.ac.id/index.php/kovalen/article/view/15324 |
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