Enhanced platelet adhesion in essential thrombocythemia after in vitro activation
OBJECTIVE: Essential thrombocythemia (ET) is a chronic myeloproliferative disorder characterized by elevated platelet counts and increased risk of thrombosis. Ex vivo data suggest increased platelet reactivity in agreement with the increased thrombosis risk, while in vitro tests often detect decreas...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Galenos Publishing House
2010-06-01
|
| Series: | Turkish Journal of Hematology |
| Subjects: | |
| Online Access: | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-82612 |
| _version_ | 1797912439624302592 |
|---|---|
| author | Andreas C. Eriksson Kourosh Lotfi Per A. Whiss |
| author_facet | Andreas C. Eriksson Kourosh Lotfi Per A. Whiss |
| author_sort | Andreas C. Eriksson |
| collection | DOAJ |
| description | OBJECTIVE: Essential thrombocythemia (ET) is a chronic myeloproliferative disorder characterized by elevated platelet counts and increased risk of thrombosis. Ex vivo data suggest increased platelet reactivity in agreement with the increased thrombosis risk, while in vitro tests often detect decreased platelet activity. The present study aimed to investigate adhesion of ET-platelets in vitro, which is an aspect of platelet function that has been addressed in only a few studies on ET patients. METHODS: The study included 30 ET patients and 14 healthy controls. Platelet adhesion was measured with a static platelet adhesion assay. RESULTS: The main finding was that ET-platelets were more readily activated by adhesion-inducing stimuli in vitro than control platelets. This was particularly evident in elderly patients and when using multiple stimuli, such as surfaces of collagen or fibrinogen combined with addition of adenosine 5'-diphosphate or ristocetin. Such multiple stimuli resulted in adhesion above the control mean +2 standard deviations for approximately 50% of the patients. CONCLUSION: The results are in accordance with the concept of increased platelet activity in ET, but opposite to most other in vitro studies. We suggest that the conditions in the adhesion assay might mimic the in vivo situation regarding the presence of chronic platelet activation. |
| first_indexed | 2024-04-10T11:56:03Z |
| format | Article |
| id | doaj.art-8fecb786975a41ad8abf3b3fcf518078 |
| institution | Directory Open Access Journal |
| issn | 1308-5263 |
| language | English |
| last_indexed | 2024-04-10T11:56:03Z |
| publishDate | 2010-06-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Turkish Journal of Hematology |
| spelling | doaj.art-8fecb786975a41ad8abf3b3fcf5180782023-02-15T16:16:48ZengGalenos Publishing HouseTurkish Journal of Hematology1308-52632010-06-01272829010.5152/tjh.2010.05TJH-82612Enhanced platelet adhesion in essential thrombocythemia after in vitro activationAndreas C. Eriksson0Kourosh Lotfi1Per A. Whiss2Division Of Drug Research/pharmacology, Department Of Medical And Health Sciences, Linköping University, Sweden And Department Of Hematology, University Hospital, Linköping, SwedenDivision Of Drug Research/clinical Pharmacology, Department Of Medical And Health Sciences, Linköping University, SwedenDivision Of Drug Research/pharmacology, Department Of Medical And Health Sciences, Linköping University, Sweden And Department Of Hematology, University Hospital, Linköping, SwedenOBJECTIVE: Essential thrombocythemia (ET) is a chronic myeloproliferative disorder characterized by elevated platelet counts and increased risk of thrombosis. Ex vivo data suggest increased platelet reactivity in agreement with the increased thrombosis risk, while in vitro tests often detect decreased platelet activity. The present study aimed to investigate adhesion of ET-platelets in vitro, which is an aspect of platelet function that has been addressed in only a few studies on ET patients. METHODS: The study included 30 ET patients and 14 healthy controls. Platelet adhesion was measured with a static platelet adhesion assay. RESULTS: The main finding was that ET-platelets were more readily activated by adhesion-inducing stimuli in vitro than control platelets. This was particularly evident in elderly patients and when using multiple stimuli, such as surfaces of collagen or fibrinogen combined with addition of adenosine 5'-diphosphate or ristocetin. Such multiple stimuli resulted in adhesion above the control mean +2 standard deviations for approximately 50% of the patients. CONCLUSION: The results are in accordance with the concept of increased platelet activity in ET, but opposite to most other in vitro studies. We suggest that the conditions in the adhesion assay might mimic the in vivo situation regarding the presence of chronic platelet activation.https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-82612essential thrombocythemiaplatelet activationadhesionthrombosisplatelet assay |
| spellingShingle | Andreas C. Eriksson Kourosh Lotfi Per A. Whiss Enhanced platelet adhesion in essential thrombocythemia after in vitro activation Turkish Journal of Hematology essential thrombocythemia platelet activation adhesion thrombosis platelet assay |
| title | Enhanced platelet adhesion in essential thrombocythemia after in vitro activation |
| title_full | Enhanced platelet adhesion in essential thrombocythemia after in vitro activation |
| title_fullStr | Enhanced platelet adhesion in essential thrombocythemia after in vitro activation |
| title_full_unstemmed | Enhanced platelet adhesion in essential thrombocythemia after in vitro activation |
| title_short | Enhanced platelet adhesion in essential thrombocythemia after in vitro activation |
| title_sort | enhanced platelet adhesion in essential thrombocythemia after in vitro activation |
| topic | essential thrombocythemia platelet activation adhesion thrombosis platelet assay |
| url | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-82612 |
| work_keys_str_mv | AT andreasceriksson enhancedplateletadhesioninessentialthrombocythemiaafterinvitroactivation AT kouroshlotfi enhancedplateletadhesioninessentialthrombocythemiaafterinvitroactivation AT perawhiss enhancedplateletadhesioninessentialthrombocythemiaafterinvitroactivation |