Genomic Analysis of the Glutathione <i>S</i>-Transferase Family in Pear (<i>Pyrus communis</i>) and Functional Identification of <i>PcGST57</i> in Anthocyanin Accumulation
Anthocyanin accumulation in vacuoles results in red coloration in pear peels. Glutathione <i>S</i>-transferase (GST) proteins have emerged as important regulators of anthocyanin accumulation. Here, a total of 57 PcGST genes were identified in the European pear ‘Bartlett’ (<i>Pyrus...
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MDPI AG
2022-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/2/746 |
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| author | Bo Li Xiangzhan Zhang Ruiwei Duan Chunhong Han Jian Yang Long Wang Suke Wang Yanli Su Lei Wang Yongfei Dong Huabai Xue |
| author_facet | Bo Li Xiangzhan Zhang Ruiwei Duan Chunhong Han Jian Yang Long Wang Suke Wang Yanli Su Lei Wang Yongfei Dong Huabai Xue |
| author_sort | Bo Li |
| collection | DOAJ |
| description | Anthocyanin accumulation in vacuoles results in red coloration in pear peels. Glutathione <i>S</i>-transferase (GST) proteins have emerged as important regulators of anthocyanin accumulation. Here, a total of 57 PcGST genes were identified in the European pear ‘Bartlett’ (<i>Pyrus communis</i>) through comprehensive genomic analysis. Phylogenetic analysis showed that PcGST genes were divided into 10 subfamilies. The gene structure, chromosomal localization, collinearity relationship, <i>cis</i>-elements in the promoter region, and conserved motifs of PcGST genes were analyzed. Further research indicated that glutamic acid (Glu) can significantly improve anthocyanin accumulation in pear peels. RNA sequencing (RNA-seq) analysis showed that Glu induced the expression of most PcGST genes, among which <i>PcGST57</i> was most significantly induced. Further phylogenetic analysis indicated that <i>PcGST57</i> was closely related to GST genes identified in other species, which were involved in anthocyanin accumulation. Transcript analysis indicated that <i>PcGST57</i> was expressed in various tissues, other than flesh, and associated with peel coloration at different developmental stages. Silencing of <i>PcGST57</i> by virus-induced gene silencing (VIGS) inhibited the expression of <i>PcGST57</i> and reduced the anthocyanin content in pear fruit. In contrast, overexpression of <i>PcGST57</i> improved anthocyanin accumulation. Collectively, our results demonstrated that <i>PcGST57</i> was involved in anthocyanin accumulation in pear and provided candidate genes for red pear breeding. |
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| last_indexed | 2024-03-10T01:19:09Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8fecfbdb3809432f8ff4b1b36852d7612023-11-23T14:03:39ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-01-0123274610.3390/ijms23020746Genomic Analysis of the Glutathione <i>S</i>-Transferase Family in Pear (<i>Pyrus communis</i>) and Functional Identification of <i>PcGST57</i> in Anthocyanin AccumulationBo Li0Xiangzhan Zhang1Ruiwei Duan2Chunhong Han3Jian Yang4Long Wang5Suke Wang6Yanli Su7Lei Wang8Yongfei Dong9Huabai Xue10Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaAnthocyanin accumulation in vacuoles results in red coloration in pear peels. Glutathione <i>S</i>-transferase (GST) proteins have emerged as important regulators of anthocyanin accumulation. Here, a total of 57 PcGST genes were identified in the European pear ‘Bartlett’ (<i>Pyrus communis</i>) through comprehensive genomic analysis. Phylogenetic analysis showed that PcGST genes were divided into 10 subfamilies. The gene structure, chromosomal localization, collinearity relationship, <i>cis</i>-elements in the promoter region, and conserved motifs of PcGST genes were analyzed. Further research indicated that glutamic acid (Glu) can significantly improve anthocyanin accumulation in pear peels. RNA sequencing (RNA-seq) analysis showed that Glu induced the expression of most PcGST genes, among which <i>PcGST57</i> was most significantly induced. Further phylogenetic analysis indicated that <i>PcGST57</i> was closely related to GST genes identified in other species, which were involved in anthocyanin accumulation. Transcript analysis indicated that <i>PcGST57</i> was expressed in various tissues, other than flesh, and associated with peel coloration at different developmental stages. Silencing of <i>PcGST57</i> by virus-induced gene silencing (VIGS) inhibited the expression of <i>PcGST57</i> and reduced the anthocyanin content in pear fruit. In contrast, overexpression of <i>PcGST57</i> improved anthocyanin accumulation. Collectively, our results demonstrated that <i>PcGST57</i> was involved in anthocyanin accumulation in pear and provided candidate genes for red pear breeding.https://www.mdpi.com/1422-0067/23/2/746pear (<i>Pyrus communis</i>)glutathione <i>S</i>-transferaseanthocyanins<i>PcGST57</i> |
| spellingShingle | Bo Li Xiangzhan Zhang Ruiwei Duan Chunhong Han Jian Yang Long Wang Suke Wang Yanli Su Lei Wang Yongfei Dong Huabai Xue Genomic Analysis of the Glutathione <i>S</i>-Transferase Family in Pear (<i>Pyrus communis</i>) and Functional Identification of <i>PcGST57</i> in Anthocyanin Accumulation International Journal of Molecular Sciences pear (<i>Pyrus communis</i>) glutathione <i>S</i>-transferase anthocyanins <i>PcGST57</i> |
| title | Genomic Analysis of the Glutathione <i>S</i>-Transferase Family in Pear (<i>Pyrus communis</i>) and Functional Identification of <i>PcGST57</i> in Anthocyanin Accumulation |
| title_full | Genomic Analysis of the Glutathione <i>S</i>-Transferase Family in Pear (<i>Pyrus communis</i>) and Functional Identification of <i>PcGST57</i> in Anthocyanin Accumulation |
| title_fullStr | Genomic Analysis of the Glutathione <i>S</i>-Transferase Family in Pear (<i>Pyrus communis</i>) and Functional Identification of <i>PcGST57</i> in Anthocyanin Accumulation |
| title_full_unstemmed | Genomic Analysis of the Glutathione <i>S</i>-Transferase Family in Pear (<i>Pyrus communis</i>) and Functional Identification of <i>PcGST57</i> in Anthocyanin Accumulation |
| title_short | Genomic Analysis of the Glutathione <i>S</i>-Transferase Family in Pear (<i>Pyrus communis</i>) and Functional Identification of <i>PcGST57</i> in Anthocyanin Accumulation |
| title_sort | genomic analysis of the glutathione i s i transferase family in pear i pyrus communis i and functional identification of i pcgst57 i in anthocyanin accumulation |
| topic | pear (<i>Pyrus communis</i>) glutathione <i>S</i>-transferase anthocyanins <i>PcGST57</i> |
| url | https://www.mdpi.com/1422-0067/23/2/746 |
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