Quercetin and Coumarin Inhibit Dipeptidyl Peptidase-IV and Exhibits Antioxidant Properties: In Silico, In Vitro, Ex Vivo
Quercetin and coumarin, two naturally occurring phytochemicals of plant origin, are known to regulate hyperglycemia and oxidative stress. The present study was designed to evaluate the inhibitory activity of quercetin and coumarin on dipeptidyl peptidase-IV (DPP-IV) and their antioxidant potential....
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MDPI AG
2020-01-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/2/207 |
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| author | Anand-Krishna Singh Pankaj Kumar Patel Komal Choudhary Jaya Joshi Dhananjay Yadav Jun-O Jin |
| author_facet | Anand-Krishna Singh Pankaj Kumar Patel Komal Choudhary Jaya Joshi Dhananjay Yadav Jun-O Jin |
| author_sort | Anand-Krishna Singh |
| collection | DOAJ |
| description | Quercetin and coumarin, two naturally occurring phytochemicals of plant origin, are known to regulate hyperglycemia and oxidative stress. The present study was designed to evaluate the inhibitory activity of quercetin and coumarin on dipeptidyl peptidase-IV (DPP-IV) and their antioxidant potential. DPP-IV inhibition assays were performed, and evaluated IC<sub>50</sub> values of diprotin A, quercetin, coumarin, and sitagliptin were found to be 0.653, 4.02, 54.83, and 5.49 nmol/mL, respectively. Furthermore, in silico studies such as the drug-likeliness and docking efficiency of quercetin and coumarin to the DPP-IV protein were performed; the ex vivo antiperoxidative potential of quercetin and coumarin were also evaluated. The results of the present study showed that the DPP-IV inhibitory potential of quercetin was slightly higher than that of sitagliptin. Virtual docking revealed the tight binding of quercetin with DPP-IV protein. Quercetin and coumarin reduced oxidative stress in vitro and ex vivo systems. We report for the first time that both compounds inhibited the DPP-IV along with antioxidant activity and thus may be use as function food ingredients in the prevention of diabetes. |
| first_indexed | 2024-12-13T07:48:46Z |
| format | Article |
| id | doaj.art-8fede49f73f74844a474c95c71b03f34 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-13T07:48:46Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-8fede49f73f74844a474c95c71b03f342022-12-21T23:54:44ZengMDPI AGBiomolecules2218-273X2020-01-0110220710.3390/biom10020207biom10020207Quercetin and Coumarin Inhibit Dipeptidyl Peptidase-IV and Exhibits Antioxidant Properties: In Silico, In Vitro, Ex VivoAnand-Krishna Singh0Pankaj Kumar Patel1Komal Choudhary2Jaya Joshi3Dhananjay Yadav4Jun-O Jin5Institute of Life Science, Shri Vaishnav Vidyapeeth Vishwavidyalaya, Indore 452111, IndiaSchool of Life Sciences, Devi Ahilya University, Indore, Madhya Pradesh 452001, IndiaSchool of Life Sciences, Devi Ahilya University, Indore, Madhya Pradesh 452001, IndiaOral Pathology and Microbiology, Government Dental College, Indore, Madhya Pradesh 452001, IndiaDepartment of Medical Biotechnology, Yeungnam University, Gyeongsan 712-749, KoreaDepartment of Medical Biotechnology, Yeungnam University, Gyeongsan 712-749, KoreaQuercetin and coumarin, two naturally occurring phytochemicals of plant origin, are known to regulate hyperglycemia and oxidative stress. The present study was designed to evaluate the inhibitory activity of quercetin and coumarin on dipeptidyl peptidase-IV (DPP-IV) and their antioxidant potential. DPP-IV inhibition assays were performed, and evaluated IC<sub>50</sub> values of diprotin A, quercetin, coumarin, and sitagliptin were found to be 0.653, 4.02, 54.83, and 5.49 nmol/mL, respectively. Furthermore, in silico studies such as the drug-likeliness and docking efficiency of quercetin and coumarin to the DPP-IV protein were performed; the ex vivo antiperoxidative potential of quercetin and coumarin were also evaluated. The results of the present study showed that the DPP-IV inhibitory potential of quercetin was slightly higher than that of sitagliptin. Virtual docking revealed the tight binding of quercetin with DPP-IV protein. Quercetin and coumarin reduced oxidative stress in vitro and ex vivo systems. We report for the first time that both compounds inhibited the DPP-IV along with antioxidant activity and thus may be use as function food ingredients in the prevention of diabetes.https://www.mdpi.com/2218-273X/10/2/207antiperoxidativecoumarindiabetesdipeptidyl peptidase-ivhyperglycemiaquercetin |
| spellingShingle | Anand-Krishna Singh Pankaj Kumar Patel Komal Choudhary Jaya Joshi Dhananjay Yadav Jun-O Jin Quercetin and Coumarin Inhibit Dipeptidyl Peptidase-IV and Exhibits Antioxidant Properties: In Silico, In Vitro, Ex Vivo Biomolecules antiperoxidative coumarin diabetes dipeptidyl peptidase-iv hyperglycemia quercetin |
| title | Quercetin and Coumarin Inhibit Dipeptidyl Peptidase-IV and Exhibits Antioxidant Properties: In Silico, In Vitro, Ex Vivo |
| title_full | Quercetin and Coumarin Inhibit Dipeptidyl Peptidase-IV and Exhibits Antioxidant Properties: In Silico, In Vitro, Ex Vivo |
| title_fullStr | Quercetin and Coumarin Inhibit Dipeptidyl Peptidase-IV and Exhibits Antioxidant Properties: In Silico, In Vitro, Ex Vivo |
| title_full_unstemmed | Quercetin and Coumarin Inhibit Dipeptidyl Peptidase-IV and Exhibits Antioxidant Properties: In Silico, In Vitro, Ex Vivo |
| title_short | Quercetin and Coumarin Inhibit Dipeptidyl Peptidase-IV and Exhibits Antioxidant Properties: In Silico, In Vitro, Ex Vivo |
| title_sort | quercetin and coumarin inhibit dipeptidyl peptidase iv and exhibits antioxidant properties in silico in vitro ex vivo |
| topic | antiperoxidative coumarin diabetes dipeptidyl peptidase-iv hyperglycemia quercetin |
| url | https://www.mdpi.com/2218-273X/10/2/207 |
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