Measurement of cholic acid synthesis and secretion by isolated rat hepatocytes.
Liver cells isolated from normal and cholestyramine-treated rats were incubated as cell suspensions for up to 4 hr in a simple, defined medium. The bile acid concentration in cells plus cell medium was determined by gas-liquid chromatography. Normal hepatocytes synthesized cholic acid at an initial...
Main Authors: | M J Whiting, A M Edwards |
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Format: | Article |
Language: | English |
Published: |
Elsevier
1979-09-01
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Series: | Journal of Lipid Research |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520400227 |
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