Assessment of Neutrophil Chemotaxis, Phagocytosis and Specific Granule Release in Aggressive and Chronic Periodontitis Patients: An In-Vitro Study
Introduction: Neutrophils are responsible for immunologically induced tissue injury. When normal regulatory mechanisms fail, following activation by bacterial by-products or other immune stimuli, neutrophils execute several specialized functions that include chemotaxis, phagocytosis, generation of r...
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| Format: | Article |
| Language: | English |
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Sonali Sharma on behalf of Rajasthan University of Health Sciences
2023-10-01
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| Series: | RUHS Journal of Health Sciences |
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| Online Access: | https://ruhsjhs.in/articleDetails.php?artid=TWpNNQ== |
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| author | Priyanka M Agrawal; Chanchal S Bherwani; Dnyaneshwari Gujar; Amit P Thareja; Nilima M Landge; Darshana Dalaya; Setu Mathur; Amit Chaudhari |
| author_facet | Priyanka M Agrawal; Chanchal S Bherwani; Dnyaneshwari Gujar; Amit P Thareja; Nilima M Landge; Darshana Dalaya; Setu Mathur; Amit Chaudhari |
| author_sort | Priyanka M Agrawal; Chanchal S Bherwani; Dnyaneshwari Gujar; Amit P Thareja; Nilima M Landge; Darshana Dalaya; Setu Mathur; Amit Chaudhari |
| collection | DOAJ |
| description | Introduction: Neutrophils are responsible for immunologically induced tissue injury. When normal regulatory mechanisms fail, following activation by bacterial by-products or other immune stimuli, neutrophils execute several specialized functions that include chemotaxis, phagocytosis, generation of reactive oxygen metabolites and specific granule release. The important role polymorphonuclear neutrophils (PMNs) play in optimal functioning of the immune defense system has led to speculation that a partly compromised system could severely weaken the defense mounted against a bacterial insult and permit the occurrence and progression of infections. The study was aimed to assess neutrophil function i.e. chemotaxis, phagocytosis and specific granule release in patients with aggressive and chronic periodontitis in Indian population. Methodology: 60 patients with age group varying between 13-60 years from both sex were selected and were divided into three groups: Group I — 20 Generalized Aggressive Periodontitis subjects (GAgP), Group II — 20 Generalized Chronic Periodontitis subjects (CP), Group III — 20 Control Group of periodontally healthy subjects. Venous blood samples were collected and various neutrophil functions viz. chemotaxis, phagocytosis and specific granule release were carried out using different assays such as N-Formylmethionyl-leucyl-phenylalanine (FMLP), Candidia albicans suspension, Nitroblue tetrazolium test (NBT). Results: The results of the study suggest that chemotaxis was significantly reduced in generalised aggressive periodontitis patients as compared to chronic periodontitis and periodontally healthy subjects. GAgP and CP patients showed no significant difference in phagocytosis rate as compared to periodontally healthy control group. Unstimulated NBT reduction is increased in neutrophils from patients with infection and the intracellular killing capacity of neutrophils is reduced in GAgP and CP patients. Conclusion: The assessment of neutrophil fu |
| first_indexed | 2024-03-11T20:44:29Z |
| format | Article |
| id | doaj.art-8fef147c621140f6aade3fa3fa8bb7df |
| institution | Directory Open Access Journal |
| issn | 2456-8309 2582-3590 |
| language | English |
| last_indexed | 2024-03-11T20:44:29Z |
| publishDate | 2023-10-01 |
| publisher | Sonali Sharma on behalf of Rajasthan University of Health Sciences |
| record_format | Article |
| series | RUHS Journal of Health Sciences |
| spelling | doaj.art-8fef147c621140f6aade3fa3fa8bb7df2023-10-01T18:53:12ZengSonali Sharma on behalf of Rajasthan University of Health SciencesRUHS Journal of Health Sciences2456-83092582-35902023-10-01http://dx.doi.org/10.37821/ruhsjhs.2.2.2017.71-76Assessment of Neutrophil Chemotaxis, Phagocytosis and Specific Granule Release in Aggressive and Chronic Periodontitis Patients: An In-Vitro StudyPriyanka M Agrawal; Chanchal S Bherwani; Dnyaneshwari Gujar; Amit P Thareja; Nilima M Landge; Darshana Dalaya; Setu Mathur; Amit Chaudhari0Assistant Professor, Department of Periodontology, BVDU Dental College and Hospital, Pune; Associate Professor, Department of Periodontology, Dr D Y Patil Dental College and Hospital, Navi Mumbai; Associate Professor, Department of Periodontology, D Y Patil Dental College and Hospital, Pune; Associate Professor, Department of Prosthodontics, BVDU Dental College & Hospital, Pune; Assistant Professor, Department of Periodontics, RUHS College of Dental Sciences, Jaipur; Associate Professor, Department of Periodontics, BVDU Dental College & Hospital, Pune.Introduction: Neutrophils are responsible for immunologically induced tissue injury. When normal regulatory mechanisms fail, following activation by bacterial by-products or other immune stimuli, neutrophils execute several specialized functions that include chemotaxis, phagocytosis, generation of reactive oxygen metabolites and specific granule release. The important role polymorphonuclear neutrophils (PMNs) play in optimal functioning of the immune defense system has led to speculation that a partly compromised system could severely weaken the defense mounted against a bacterial insult and permit the occurrence and progression of infections. The study was aimed to assess neutrophil function i.e. chemotaxis, phagocytosis and specific granule release in patients with aggressive and chronic periodontitis in Indian population. Methodology: 60 patients with age group varying between 13-60 years from both sex were selected and were divided into three groups: Group I — 20 Generalized Aggressive Periodontitis subjects (GAgP), Group II — 20 Generalized Chronic Periodontitis subjects (CP), Group III — 20 Control Group of periodontally healthy subjects. Venous blood samples were collected and various neutrophil functions viz. chemotaxis, phagocytosis and specific granule release were carried out using different assays such as N-Formylmethionyl-leucyl-phenylalanine (FMLP), Candidia albicans suspension, Nitroblue tetrazolium test (NBT). Results: The results of the study suggest that chemotaxis was significantly reduced in generalised aggressive periodontitis patients as compared to chronic periodontitis and periodontally healthy subjects. GAgP and CP patients showed no significant difference in phagocytosis rate as compared to periodontally healthy control group. Unstimulated NBT reduction is increased in neutrophils from patients with infection and the intracellular killing capacity of neutrophils is reduced in GAgP and CP patients. Conclusion: The assessment of neutrophil fuhttps://ruhsjhs.in/articleDetails.php?artid=TWpNNQ==Not available |
| spellingShingle | Priyanka M Agrawal; Chanchal S Bherwani; Dnyaneshwari Gujar; Amit P Thareja; Nilima M Landge; Darshana Dalaya; Setu Mathur; Amit Chaudhari Assessment of Neutrophil Chemotaxis, Phagocytosis and Specific Granule Release in Aggressive and Chronic Periodontitis Patients: An In-Vitro Study RUHS Journal of Health Sciences Not available |
| title | Assessment of Neutrophil Chemotaxis, Phagocytosis and Specific Granule Release in Aggressive and Chronic Periodontitis Patients: An In-Vitro Study |
| title_full | Assessment of Neutrophil Chemotaxis, Phagocytosis and Specific Granule Release in Aggressive and Chronic Periodontitis Patients: An In-Vitro Study |
| title_fullStr | Assessment of Neutrophil Chemotaxis, Phagocytosis and Specific Granule Release in Aggressive and Chronic Periodontitis Patients: An In-Vitro Study |
| title_full_unstemmed | Assessment of Neutrophil Chemotaxis, Phagocytosis and Specific Granule Release in Aggressive and Chronic Periodontitis Patients: An In-Vitro Study |
| title_short | Assessment of Neutrophil Chemotaxis, Phagocytosis and Specific Granule Release in Aggressive and Chronic Periodontitis Patients: An In-Vitro Study |
| title_sort | assessment of neutrophil chemotaxis phagocytosis and specific granule release in aggressive and chronic periodontitis patients an in vitro study |
| topic | Not available |
| url | https://ruhsjhs.in/articleDetails.php?artid=TWpNNQ== |
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