<i>Bartonella </i>spp. Prevalence (Serology, Culture, and PCR) in Sanitary Workers in La Rioja Spain
<i>Bartonella </i>spp. are increasingly implicated in association with a spectrum of zoonotic infectious diseases. One hundred sanitary workers in La Rioja, Spain completed a questionnaire and provided blood specimens for <i>Bartonella </i>spp. serology and <i>Bartonell...
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| Format: | Article |
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MDPI AG
2020-03-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/3/189 |
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| author | Aránzazu Portillo Ricardo Maggi José A. Oteo Julie Bradley Lara García-Álvarez Montserrat San-Martín Xavier Roura Edward Breitschwerdt |
| author_facet | Aránzazu Portillo Ricardo Maggi José A. Oteo Julie Bradley Lara García-Álvarez Montserrat San-Martín Xavier Roura Edward Breitschwerdt |
| author_sort | Aránzazu Portillo |
| collection | DOAJ |
| description | <i>Bartonella </i>spp. are increasingly implicated in association with a spectrum of zoonotic infectious diseases. One hundred sanitary workers in La Rioja, Spain completed a questionnaire and provided blood specimens for <i>Bartonella </i>spp. serology and <i>Bartonella </i>alpha-Proteobacteria growth medium (BAPGM) enrichment blood culture/PCR. Six immunofluorescence assays (IFA) were performed and aseptically obtained blood specimens were inoculated into liquid BAPGM and subcultured onto blood agar plates. <i>Bartonella </i>DNA was amplified using conventional and real-time PCR assays. The <i>Bartonella </i>spp., strain, or genotype was determined by DNA sequencing. <i>Bartonella </i>seroreactivity was documented in 83.1% and bloodstream infection in 21.6% of participants. <i>Bartonella henselae</i>, <i>B. vinsonii </i>subsp. <i>berkhoffii </i>genotypes I and III, and <i>B. quintana </i>were identified. IFA seroreactivity and PCR positivity were not statistically associated with self-reported symptoms. Our results suggest that exposure to and non-clinical infection with <i>Bartonella </i>spp. may occur more often than previously suspected in the La Rioja region. |
| first_indexed | 2024-04-11T22:13:48Z |
| format | Article |
| id | doaj.art-8fefb598bf9146759cc6fa6e221594c9 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-04-11T22:13:48Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-8fefb598bf9146759cc6fa6e221594c92022-12-22T04:00:28ZengMDPI AGPathogens2076-08172020-03-019318910.3390/pathogens9030189pathogens9030189<i>Bartonella </i>spp. Prevalence (Serology, Culture, and PCR) in Sanitary Workers in La Rioja SpainAránzazu Portillo0Ricardo Maggi1José A. Oteo2Julie Bradley3Lara García-Álvarez4Montserrat San-Martín5Xavier Roura6Edward Breitschwerdt7Departamento de Enfermedades Infecciosas, Hospital U. San Pedro-Centro de Investigación Biomédica de La Rioja (CIBIR), 26006 Logroño, La Rioja, SpainGalaxy Diagnostics, Research Triangle Park, Morrisville, NC 27709, USADepartamento de Enfermedades Infecciosas, Hospital U. San Pedro-Centro de Investigación Biomédica de La Rioja (CIBIR), 26006 Logroño, La Rioja, SpainIntracellular Pathogens Research Laboratory, Comparative Medicine Institute, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27695, USADepartamento de Enfermedades Infecciosas, Hospital U. San Pedro-Centro de Investigación Biomédica de La Rioja (CIBIR), 26006 Logroño, La Rioja, SpainFaculty of Social Sciences, University of Granada, 52006 Melilla, SpainHospital Clínic Veterinari, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, SpainGalaxy Diagnostics, Research Triangle Park, Morrisville, NC 27709, USA<i>Bartonella </i>spp. are increasingly implicated in association with a spectrum of zoonotic infectious diseases. One hundred sanitary workers in La Rioja, Spain completed a questionnaire and provided blood specimens for <i>Bartonella </i>spp. serology and <i>Bartonella </i>alpha-Proteobacteria growth medium (BAPGM) enrichment blood culture/PCR. Six immunofluorescence assays (IFA) were performed and aseptically obtained blood specimens were inoculated into liquid BAPGM and subcultured onto blood agar plates. <i>Bartonella </i>DNA was amplified using conventional and real-time PCR assays. The <i>Bartonella </i>spp., strain, or genotype was determined by DNA sequencing. <i>Bartonella </i>seroreactivity was documented in 83.1% and bloodstream infection in 21.6% of participants. <i>Bartonella henselae</i>, <i>B. vinsonii </i>subsp. <i>berkhoffii </i>genotypes I and III, and <i>B. quintana </i>were identified. IFA seroreactivity and PCR positivity were not statistically associated with self-reported symptoms. Our results suggest that exposure to and non-clinical infection with <i>Bartonella </i>spp. may occur more often than previously suspected in the La Rioja region.https://www.mdpi.com/2076-0817/9/3/189<i>bartonella </i>alpha-proteobacteria growth medium (bapgm), <i>b. henselae</i>b. quintanab. vinsonii subsp. <i>berkhoffii</i>b. koehleraesanitary workers |
| spellingShingle | Aránzazu Portillo Ricardo Maggi José A. Oteo Julie Bradley Lara García-Álvarez Montserrat San-Martín Xavier Roura Edward Breitschwerdt <i>Bartonella </i>spp. Prevalence (Serology, Culture, and PCR) in Sanitary Workers in La Rioja Spain Pathogens <i>bartonella </i>alpha-proteobacteria growth medium (bapgm), <i>b. henselae</i> b. quintana b. vinsonii subsp. <i>berkhoffii</i> b. koehlerae sanitary workers |
| title | <i>Bartonella </i>spp. Prevalence (Serology, Culture, and PCR) in Sanitary Workers in La Rioja Spain |
| title_full | <i>Bartonella </i>spp. Prevalence (Serology, Culture, and PCR) in Sanitary Workers in La Rioja Spain |
| title_fullStr | <i>Bartonella </i>spp. Prevalence (Serology, Culture, and PCR) in Sanitary Workers in La Rioja Spain |
| title_full_unstemmed | <i>Bartonella </i>spp. Prevalence (Serology, Culture, and PCR) in Sanitary Workers in La Rioja Spain |
| title_short | <i>Bartonella </i>spp. Prevalence (Serology, Culture, and PCR) in Sanitary Workers in La Rioja Spain |
| title_sort | i bartonella i spp prevalence serology culture and pcr in sanitary workers in la rioja spain |
| topic | <i>bartonella </i>alpha-proteobacteria growth medium (bapgm), <i>b. henselae</i> b. quintana b. vinsonii subsp. <i>berkhoffii</i> b. koehlerae sanitary workers |
| url | https://www.mdpi.com/2076-0817/9/3/189 |
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