Abrupt Photoperiod Changes Differentially Modulate Hepatic Antioxidant Response in Healthy and Obese Rats: Effects of Grape Seed Proanthocyanidin Extract (GSPE)
Disruptions of the light/dark cycle and unhealthy diets can promote misalignment of biological rhythms and metabolic alterations, ultimately leading to an oxidative stress condition. Grape seed proanthocyanidin extract (GSPE), which possesses antioxidant properties, has demonstrated its beneficial e...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-12-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/23/17057 |
| _version_ | 1797400057753894912 |
|---|---|
| author | Antonio J. Cortés-Espinar Néstor Ibarz-Blanch Jorge R. Soliz-Rueda Enrique Calvo Francisca Isabel Bravo Miquel Mulero Javier Ávila-Román |
| author_facet | Antonio J. Cortés-Espinar Néstor Ibarz-Blanch Jorge R. Soliz-Rueda Enrique Calvo Francisca Isabel Bravo Miquel Mulero Javier Ávila-Román |
| author_sort | Antonio J. Cortés-Espinar |
| collection | DOAJ |
| description | Disruptions of the light/dark cycle and unhealthy diets can promote misalignment of biological rhythms and metabolic alterations, ultimately leading to an oxidative stress condition. Grape seed proanthocyanidin extract (GSPE), which possesses antioxidant properties, has demonstrated its beneficial effects in metabolic-associated diseases and its potential role in modulating circadian disruptions. Therefore, this study aimed to assess the impact of GSPE administration on the liver oxidant system of healthy and diet-induced obese rats undergoing a sudden photoperiod shift. To this end, forty-eight photoperiod-sensitive Fischer 344/IcoCrl rats were fed either a standard (STD) or a cafeteria diet (CAF) for 6 weeks. A week before euthanizing, rats were abruptly transferred from a standard photoperiod of 12 h of light/day (L12) to either a short (6 h light/day, L6) or a long photoperiod (18 h light/day, L18) while receiving a daily oral dose of vehicle (VH) or GSPE (25 mg/kg). Alterations in body weight gain, serum and liver biochemical parameters, antioxidant gene and protein expression, and antioxidant metabolites were observed. Interestingly, GSPE partially ameliorated these effects by reducing the oxidative stress status in L6 through an increase in <i>GPx1</i> expression and in hepatic antioxidant metabolites and in L18 by increasing the NRF2/KEAP1/ARE pathway, thereby showing potential in the treatment of circadian-related disorders by increasing the hepatic antioxidant response in a photoperiod-dependent manner. |
| first_indexed | 2024-03-09T01:49:04Z |
| format | Article |
| id | doaj.art-8ff295917e404553bdc8215313caf20d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T01:49:04Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8ff295917e404553bdc8215313caf20d2023-12-08T15:18:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0124231705710.3390/ijms242317057Abrupt Photoperiod Changes Differentially Modulate Hepatic Antioxidant Response in Healthy and Obese Rats: Effects of Grape Seed Proanthocyanidin Extract (GSPE)Antonio J. Cortés-Espinar0Néstor Ibarz-Blanch1Jorge R. Soliz-Rueda2Enrique Calvo3Francisca Isabel Bravo4Miquel Mulero5Javier Ávila-Román6Nutrigenomics Research Group, Department of Biochemistry and Biotechnology, Universitat Rovira i Virgili, 43007 Tarragona, SpainNutrigenomics Research Group, Department of Biochemistry and Biotechnology, Universitat Rovira i Virgili, 43007 Tarragona, SpainNutrigenomics Research Group, Department of Biochemistry and Biotechnology, Universitat Rovira i Virgili, 43007 Tarragona, SpainNutrigenomics Research Group, Department of Biochemistry and Biotechnology, Universitat Rovira i Virgili, 43007 Tarragona, SpainNutrigenomics Research Group, Department of Biochemistry and Biotechnology, Universitat Rovira i Virgili, 43007 Tarragona, SpainNutrigenomics Research Group, Department of Biochemistry and Biotechnology, Universitat Rovira i Virgili, 43007 Tarragona, SpainMolecular and Applied Pharmacology Group (FARMOLAP), Department of Pharmacology, Universidad de Sevilla, 41012 Sevilla, SpainDisruptions of the light/dark cycle and unhealthy diets can promote misalignment of biological rhythms and metabolic alterations, ultimately leading to an oxidative stress condition. Grape seed proanthocyanidin extract (GSPE), which possesses antioxidant properties, has demonstrated its beneficial effects in metabolic-associated diseases and its potential role in modulating circadian disruptions. Therefore, this study aimed to assess the impact of GSPE administration on the liver oxidant system of healthy and diet-induced obese rats undergoing a sudden photoperiod shift. To this end, forty-eight photoperiod-sensitive Fischer 344/IcoCrl rats were fed either a standard (STD) or a cafeteria diet (CAF) for 6 weeks. A week before euthanizing, rats were abruptly transferred from a standard photoperiod of 12 h of light/day (L12) to either a short (6 h light/day, L6) or a long photoperiod (18 h light/day, L18) while receiving a daily oral dose of vehicle (VH) or GSPE (25 mg/kg). Alterations in body weight gain, serum and liver biochemical parameters, antioxidant gene and protein expression, and antioxidant metabolites were observed. Interestingly, GSPE partially ameliorated these effects by reducing the oxidative stress status in L6 through an increase in <i>GPx1</i> expression and in hepatic antioxidant metabolites and in L18 by increasing the NRF2/KEAP1/ARE pathway, thereby showing potential in the treatment of circadian-related disorders by increasing the hepatic antioxidant response in a photoperiod-dependent manner.https://www.mdpi.com/1422-0067/24/23/17057oxidative stressphenolic compoundsobesityGSPEchronodisruptionliver |
| spellingShingle | Antonio J. Cortés-Espinar Néstor Ibarz-Blanch Jorge R. Soliz-Rueda Enrique Calvo Francisca Isabel Bravo Miquel Mulero Javier Ávila-Román Abrupt Photoperiod Changes Differentially Modulate Hepatic Antioxidant Response in Healthy and Obese Rats: Effects of Grape Seed Proanthocyanidin Extract (GSPE) International Journal of Molecular Sciences oxidative stress phenolic compounds obesity GSPE chronodisruption liver |
| title | Abrupt Photoperiod Changes Differentially Modulate Hepatic Antioxidant Response in Healthy and Obese Rats: Effects of Grape Seed Proanthocyanidin Extract (GSPE) |
| title_full | Abrupt Photoperiod Changes Differentially Modulate Hepatic Antioxidant Response in Healthy and Obese Rats: Effects of Grape Seed Proanthocyanidin Extract (GSPE) |
| title_fullStr | Abrupt Photoperiod Changes Differentially Modulate Hepatic Antioxidant Response in Healthy and Obese Rats: Effects of Grape Seed Proanthocyanidin Extract (GSPE) |
| title_full_unstemmed | Abrupt Photoperiod Changes Differentially Modulate Hepatic Antioxidant Response in Healthy and Obese Rats: Effects of Grape Seed Proanthocyanidin Extract (GSPE) |
| title_short | Abrupt Photoperiod Changes Differentially Modulate Hepatic Antioxidant Response in Healthy and Obese Rats: Effects of Grape Seed Proanthocyanidin Extract (GSPE) |
| title_sort | abrupt photoperiod changes differentially modulate hepatic antioxidant response in healthy and obese rats effects of grape seed proanthocyanidin extract gspe |
| topic | oxidative stress phenolic compounds obesity GSPE chronodisruption liver |
| url | https://www.mdpi.com/1422-0067/24/23/17057 |
| work_keys_str_mv | AT antoniojcortesespinar abruptphotoperiodchangesdifferentiallymodulatehepaticantioxidantresponseinhealthyandobeseratseffectsofgrapeseedproanthocyanidinextractgspe AT nestoribarzblanch abruptphotoperiodchangesdifferentiallymodulatehepaticantioxidantresponseinhealthyandobeseratseffectsofgrapeseedproanthocyanidinextractgspe AT jorgersolizrueda abruptphotoperiodchangesdifferentiallymodulatehepaticantioxidantresponseinhealthyandobeseratseffectsofgrapeseedproanthocyanidinextractgspe AT enriquecalvo abruptphotoperiodchangesdifferentiallymodulatehepaticantioxidantresponseinhealthyandobeseratseffectsofgrapeseedproanthocyanidinextractgspe AT franciscaisabelbravo abruptphotoperiodchangesdifferentiallymodulatehepaticantioxidantresponseinhealthyandobeseratseffectsofgrapeseedproanthocyanidinextractgspe AT miquelmulero abruptphotoperiodchangesdifferentiallymodulatehepaticantioxidantresponseinhealthyandobeseratseffectsofgrapeseedproanthocyanidinextractgspe AT javieravilaroman abruptphotoperiodchangesdifferentiallymodulatehepaticantioxidantresponseinhealthyandobeseratseffectsofgrapeseedproanthocyanidinextractgspe |