Nano-encapsulation of curcumin in fish collagen grafted succinyl chitosan hydrogel accelerates wound healing process in experimental rats
Succinyl chitosan-fish collagen composite hydrogel containing nano-encapsulated curcumin was developed for wound healing application. Curcumin was encapsulated in succinyl chitosan nano-particles using ionic gelation technique. The Z-average diameter of the nanoparticles as observed by dynamic light...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Food Hydrocolloids for Health |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667025922000097 |
| _version_ | 1811207997154656256 |
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| author | Niladri Sekhar Chatterjee Hema Girija Sukumaran Pavan Kumar Dara Balaraman Ganesan Muhamed Ashraf Rangasamy Anandan Suseela Mathew Ravishankar Chandragiri Nagarajarao |
| author_facet | Niladri Sekhar Chatterjee Hema Girija Sukumaran Pavan Kumar Dara Balaraman Ganesan Muhamed Ashraf Rangasamy Anandan Suseela Mathew Ravishankar Chandragiri Nagarajarao |
| author_sort | Niladri Sekhar Chatterjee |
| collection | DOAJ |
| description | Succinyl chitosan-fish collagen composite hydrogel containing nano-encapsulated curcumin was developed for wound healing application. Curcumin was encapsulated in succinyl chitosan nano-particles using ionic gelation technique. The Z-average diameter of the nanoparticles as observed by dynamic light scattering experiment was ∼47 nm. Similar size ranges were observed in atomic force microscope (AFM) and transmission electron microscopy (TEM) images. A simple one pot gelation of the composite hydrogel containing nano-encapsulated curcumin was achieved by crosslinking succinyl chitosan and fish collagen with caffeic acid. The scanning electron microscopy (SEM) images of the crosslinked hydrogel showed porous sheet like/spongy morphology typical to crosslinked hydrogels. The developed hydrogel was evaluated for its wound healing efficacy in subcutaneous wound model on Wistar albino rats. The hydrogel significantly enhanced the collagen deposition and hydroxyproline content of 599.4 μg/gm and 74.9 μg/gm in wound tissue on the 14th day of post wounding. |
| first_indexed | 2024-04-12T04:14:32Z |
| format | Article |
| id | doaj.art-8ff6022fe1b94e1494354a61777c41d3 |
| institution | Directory Open Access Journal |
| issn | 2667-0259 |
| language | English |
| last_indexed | 2024-04-12T04:14:32Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Hydrocolloids for Health |
| spelling | doaj.art-8ff6022fe1b94e1494354a61777c41d32022-12-22T03:48:26ZengElsevierFood Hydrocolloids for Health2667-02592022-12-012100061Nano-encapsulation of curcumin in fish collagen grafted succinyl chitosan hydrogel accelerates wound healing process in experimental ratsNiladri Sekhar Chatterjee0Hema Girija Sukumaran1Pavan Kumar Dara2Balaraman Ganesan3Muhamed Ashraf4Rangasamy Anandan5Suseela Mathew6Ravishankar Chandragiri Nagarajarao7Biochemistry and Nutrition Division, ICAR- Central Institute of Fisheries Technology, Cochin, Kerala 682029, IndiaDepartment of Biotechnology, Mar Ivanios College, Thiruvananthapuram, Kerala 695015, IndiaBiochemistry and Nutrition Division, ICAR- Central Institute of Fisheries Technology, Cochin, Kerala 682029, IndiaBiochemistry and Nutrition Division, ICAR- Central Institute of Fisheries Technology, Cochin, Kerala 682029, IndiaBiochemistry and Nutrition Division, ICAR- Central Institute of Fisheries Technology, Cochin, Kerala 682029, IndiaBiochemistry and Nutrition Division, ICAR- Central Institute of Fisheries Technology, Cochin, Kerala 682029, India; Corresponding author.Biochemistry and Nutrition Division, ICAR- Central Institute of Fisheries Technology, Cochin, Kerala 682029, IndiaBiochemistry and Nutrition Division, ICAR- Central Institute of Fisheries Technology, Cochin, Kerala 682029, IndiaSuccinyl chitosan-fish collagen composite hydrogel containing nano-encapsulated curcumin was developed for wound healing application. Curcumin was encapsulated in succinyl chitosan nano-particles using ionic gelation technique. The Z-average diameter of the nanoparticles as observed by dynamic light scattering experiment was ∼47 nm. Similar size ranges were observed in atomic force microscope (AFM) and transmission electron microscopy (TEM) images. A simple one pot gelation of the composite hydrogel containing nano-encapsulated curcumin was achieved by crosslinking succinyl chitosan and fish collagen with caffeic acid. The scanning electron microscopy (SEM) images of the crosslinked hydrogel showed porous sheet like/spongy morphology typical to crosslinked hydrogels. The developed hydrogel was evaluated for its wound healing efficacy in subcutaneous wound model on Wistar albino rats. The hydrogel significantly enhanced the collagen deposition and hydroxyproline content of 599.4 μg/gm and 74.9 μg/gm in wound tissue on the 14th day of post wounding.http://www.sciencedirect.com/science/article/pii/S2667025922000097Succinyl chitosanFish collagenNano-encapsulationCurcuminWound healingComposite hydrogel |
| spellingShingle | Niladri Sekhar Chatterjee Hema Girija Sukumaran Pavan Kumar Dara Balaraman Ganesan Muhamed Ashraf Rangasamy Anandan Suseela Mathew Ravishankar Chandragiri Nagarajarao Nano-encapsulation of curcumin in fish collagen grafted succinyl chitosan hydrogel accelerates wound healing process in experimental rats Food Hydrocolloids for Health Succinyl chitosan Fish collagen Nano-encapsulation Curcumin Wound healing Composite hydrogel |
| title | Nano-encapsulation of curcumin in fish collagen grafted succinyl chitosan hydrogel accelerates wound healing process in experimental rats |
| title_full | Nano-encapsulation of curcumin in fish collagen grafted succinyl chitosan hydrogel accelerates wound healing process in experimental rats |
| title_fullStr | Nano-encapsulation of curcumin in fish collagen grafted succinyl chitosan hydrogel accelerates wound healing process in experimental rats |
| title_full_unstemmed | Nano-encapsulation of curcumin in fish collagen grafted succinyl chitosan hydrogel accelerates wound healing process in experimental rats |
| title_short | Nano-encapsulation of curcumin in fish collagen grafted succinyl chitosan hydrogel accelerates wound healing process in experimental rats |
| title_sort | nano encapsulation of curcumin in fish collagen grafted succinyl chitosan hydrogel accelerates wound healing process in experimental rats |
| topic | Succinyl chitosan Fish collagen Nano-encapsulation Curcumin Wound healing Composite hydrogel |
| url | http://www.sciencedirect.com/science/article/pii/S2667025922000097 |
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