Evaluation of Antibiotic Resistance Pattern of Meropenem and Piperacillin- Tazobactam in Multi Drug Resistant Acinetobacter baumannii Isolates by Flow Cytometry Method
Background and Aims: Flow cytometry is a rapid method that can analyze thousands of cells per second and can be used for determination of microbial populations and determination of bacterial antimicrobial susceptibility. In this study antibiotic resistance pattern of Acinetobacter baumannii isolates...
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| Format: | Article |
| Language: | English |
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Farname
2019-10-01
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| Series: | Iranian Journal of Medical Microbiology |
| Subjects: | |
| Online Access: | http://ijmm.ir/article-1-955-en.html |
| _version_ | 1798026856427945984 |
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| author | Nahid Rahimi Sahar Honarmand Jahromy Shohreh Zare Karizi |
| author_facet | Nahid Rahimi Sahar Honarmand Jahromy Shohreh Zare Karizi |
| author_sort | Nahid Rahimi |
| collection | DOAJ |
| description | Background and Aims: Flow cytometry is a rapid method that can analyze thousands of cells per second and can be used for determination of microbial populations and determination of bacterial antimicrobial susceptibility. In this study antibiotic resistance pattern of Acinetobacter baumannii isolates by flow cytometer was evaluated.
Materials and Methods: 55 isolates of Acinetobacter baumannii were isolated from clinical specimen of patients and were identified by biochemical tests. Antibiotic resistance patterns were studied by disc diffusion method and MDR strains were selected. MIC of Meropenem and Piperacillin were determined. Also antibiotic resistance pattern of isolates was determined by coloring with Rhodamine-123 and flow cytometry.
Results: 98% of isolates were MDR. The MIC ranges for maropenem were 8 - 256 μg/mL and for piperacillin were 128-1024 μg/mL. By flow cytometry it was demonstrated that at concentrations of 8, 4 and 2 μg/mL of meropenem, only 1.96%, 1.44% and 0.59%, of cells were killed respectively. At concentrations of 64,128 and 16 μg/mL of piperacillin, 13.8%, 11.3% and 5.9%of cells were killed respectively. Reducing the number of living bacteria was observed with increasing concentrations of both antibiotics.
Conclusion: The similarity between the results of flow cytometry and both agar and broth antibacterial susceptibility methods showed flow cytometry as a reliable and rapid test that can be used for this purpose. |
| first_indexed | 2024-04-11T18:42:12Z |
| format | Article |
| id | doaj.art-8ff618d5cd804f499a1df89800eb97cb |
| institution | Directory Open Access Journal |
| issn | 1735-8612 2345-4342 |
| language | English |
| last_indexed | 2024-04-11T18:42:12Z |
| publishDate | 2019-10-01 |
| publisher | Farname |
| record_format | Article |
| series | Iranian Journal of Medical Microbiology |
| spelling | doaj.art-8ff618d5cd804f499a1df89800eb97cb2022-12-22T04:08:57ZengFarnameIranian Journal of Medical Microbiology1735-86122345-43422019-10-01133194209Evaluation of Antibiotic Resistance Pattern of Meropenem and Piperacillin- Tazobactam in Multi Drug Resistant Acinetobacter baumannii Isolates by Flow Cytometry MethodNahid Rahimi0Sahar Honarmand Jahromy1Shohreh Zare Karizi2 Department of Microbiology, School of Biological Sciences, Varamin- Pishva Branch, Islamic Azad University, Varamin, Iran Department of Microbiology, School of Biological Sciences, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran Department of Genetics, School of Biological Sciences, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran Background and Aims: Flow cytometry is a rapid method that can analyze thousands of cells per second and can be used for determination of microbial populations and determination of bacterial antimicrobial susceptibility. In this study antibiotic resistance pattern of Acinetobacter baumannii isolates by flow cytometer was evaluated. Materials and Methods: 55 isolates of Acinetobacter baumannii were isolated from clinical specimen of patients and were identified by biochemical tests. Antibiotic resistance patterns were studied by disc diffusion method and MDR strains were selected. MIC of Meropenem and Piperacillin were determined. Also antibiotic resistance pattern of isolates was determined by coloring with Rhodamine-123 and flow cytometry. Results: 98% of isolates were MDR. The MIC ranges for maropenem were 8 - 256 μg/mL and for piperacillin were 128-1024 μg/mL. By flow cytometry it was demonstrated that at concentrations of 8, 4 and 2 μg/mL of meropenem, only 1.96%, 1.44% and 0.59%, of cells were killed respectively. At concentrations of 64,128 and 16 μg/mL of piperacillin, 13.8%, 11.3% and 5.9%of cells were killed respectively. Reducing the number of living bacteria was observed with increasing concentrations of both antibiotics. Conclusion: The similarity between the results of flow cytometry and both agar and broth antibacterial susceptibility methods showed flow cytometry as a reliable and rapid test that can be used for this purpose.http://ijmm.ir/article-1-955-en.htmlantibiotic susceptibilitymdracinetobacter baumanniiflow cytometer |
| spellingShingle | Nahid Rahimi Sahar Honarmand Jahromy Shohreh Zare Karizi Evaluation of Antibiotic Resistance Pattern of Meropenem and Piperacillin- Tazobactam in Multi Drug Resistant Acinetobacter baumannii Isolates by Flow Cytometry Method Iranian Journal of Medical Microbiology antibiotic susceptibility mdr acinetobacter baumannii flow cytometer |
| title | Evaluation of Antibiotic Resistance Pattern of Meropenem and Piperacillin- Tazobactam in Multi Drug Resistant Acinetobacter baumannii Isolates by Flow Cytometry Method |
| title_full | Evaluation of Antibiotic Resistance Pattern of Meropenem and Piperacillin- Tazobactam in Multi Drug Resistant Acinetobacter baumannii Isolates by Flow Cytometry Method |
| title_fullStr | Evaluation of Antibiotic Resistance Pattern of Meropenem and Piperacillin- Tazobactam in Multi Drug Resistant Acinetobacter baumannii Isolates by Flow Cytometry Method |
| title_full_unstemmed | Evaluation of Antibiotic Resistance Pattern of Meropenem and Piperacillin- Tazobactam in Multi Drug Resistant Acinetobacter baumannii Isolates by Flow Cytometry Method |
| title_short | Evaluation of Antibiotic Resistance Pattern of Meropenem and Piperacillin- Tazobactam in Multi Drug Resistant Acinetobacter baumannii Isolates by Flow Cytometry Method |
| title_sort | evaluation of antibiotic resistance pattern of meropenem and piperacillin tazobactam in multi drug resistant acinetobacter baumannii isolates by flow cytometry method |
| topic | antibiotic susceptibility mdr acinetobacter baumannii flow cytometer |
| url | http://ijmm.ir/article-1-955-en.html |
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