Characterization of exosomal microRNAs in preterm infants fed with breast milk and infant formula
Breastfeeding not only reduces infection-related morbidity, but also increases growth of preterm infants. Advantages of breast milk (BM) for preterm infants are significant. They continue to be studied. However, because not all preterm infants can receive breastfeeding, bovine-based infant formula (...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2024.1339919/full |
| _version_ | 1827379561601433600 |
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| author | Eun-Bit Kim Jun Hwan Song Linh Nguy-Hoang Le Ho Kim Ji Won Koh Yekyeng Seo Hwal Rim Jeong Hyun-Taek Kim Seongho Ryu |
| author_facet | Eun-Bit Kim Jun Hwan Song Linh Nguy-Hoang Le Ho Kim Ji Won Koh Yekyeng Seo Hwal Rim Jeong Hyun-Taek Kim Seongho Ryu |
| author_sort | Eun-Bit Kim |
| collection | DOAJ |
| description | Breastfeeding not only reduces infection-related morbidity, but also increases growth of preterm infants. Advantages of breast milk (BM) for preterm infants are significant. They continue to be studied. However, because not all preterm infants can receive breastfeeding, bovine-based infant formula (IF) is used as an alternative, which may increase the risk of several preterm complications. Exosomes isolated from biofluids are emerging as biomarkers in research of various diseases. Here, we characterized miRNA contents of exosomes in urine and serum samples of preterm infants who were BM and IF fed and performed transcriptomic analysis of small RNA libraries. We identified significantly up-regulated 6 miRNAs and 10 miRNAs, respectively. Gene Ontology (GO) analysis revealed that target genes of these miRNAs might participate in neuronal development, immunity modulation, detoxification of reactive oxygen species, and transmembrane exchange. Our data suggest that exosome-based systemic screening for preterm infants with breastfeeding might be a screening tool for identifying target molecules involved in therapy for preterm infants in neonatal intensive care unit (NICU) and for future application as nutraceutical formulations or pharmaceuticals. |
| first_indexed | 2024-03-08T13:18:41Z |
| format | Article |
| id | doaj.art-8ff69f9937ed4a3383d541dc65bd2772 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-03-08T13:18:41Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-8ff69f9937ed4a3383d541dc65bd27722024-01-18T04:37:11ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2024-01-011110.3389/fnut.2024.13399191339919Characterization of exosomal microRNAs in preterm infants fed with breast milk and infant formulaEun-Bit Kim0Jun Hwan Song1Linh Nguy-Hoang Le2Ho Kim3Ji Won Koh4Yekyeng Seo5Hwal Rim Jeong6Hyun-Taek Kim7Seongho Ryu8Soonchunhyang Institute of Med-bio Science (SIMS), Soonchunhyang University, Cheonan, Republic of KoreaSoonchunhyang University Cheonan Hospital, College of Medicine, Soon-chunhyang University, Cheonan, Republic of KoreaDepartment of Integrated Biomedical Science, Soonchunhyang University, Cheonan, Republic of KoreaSoonchunhyang University Cheonan Hospital, College of Medicine, Soon-chunhyang University, Cheonan, Republic of KoreaSoonchunhyang University Cheonan Hospital, College of Medicine, Soon-chunhyang University, Cheonan, Republic of KoreaSoonchunhyang University Cheonan Hospital, College of Medicine, Soon-chunhyang University, Cheonan, Republic of KoreaDepartment of Pediatrics, College of Medicine, Soonchunhyang University, Cheonan, Republic of KoreaSoonchunhyang Institute of Med-bio Science (SIMS), Soonchunhyang University, Cheonan, Republic of KoreaDepartment of Integrated Biomedical Science, Soonchunhyang University, Cheonan, Republic of KoreaBreastfeeding not only reduces infection-related morbidity, but also increases growth of preterm infants. Advantages of breast milk (BM) for preterm infants are significant. They continue to be studied. However, because not all preterm infants can receive breastfeeding, bovine-based infant formula (IF) is used as an alternative, which may increase the risk of several preterm complications. Exosomes isolated from biofluids are emerging as biomarkers in research of various diseases. Here, we characterized miRNA contents of exosomes in urine and serum samples of preterm infants who were BM and IF fed and performed transcriptomic analysis of small RNA libraries. We identified significantly up-regulated 6 miRNAs and 10 miRNAs, respectively. Gene Ontology (GO) analysis revealed that target genes of these miRNAs might participate in neuronal development, immunity modulation, detoxification of reactive oxygen species, and transmembrane exchange. Our data suggest that exosome-based systemic screening for preterm infants with breastfeeding might be a screening tool for identifying target molecules involved in therapy for preterm infants in neonatal intensive care unit (NICU) and for future application as nutraceutical formulations or pharmaceuticals.https://www.frontiersin.org/articles/10.3389/fnut.2024.1339919/fullpreterm infantexosomesmiRNAsmall RNA sequencingbreastfeedingserum |
| spellingShingle | Eun-Bit Kim Jun Hwan Song Linh Nguy-Hoang Le Ho Kim Ji Won Koh Yekyeng Seo Hwal Rim Jeong Hyun-Taek Kim Seongho Ryu Characterization of exosomal microRNAs in preterm infants fed with breast milk and infant formula Frontiers in Nutrition preterm infant exosomes miRNA small RNA sequencing breastfeeding serum |
| title | Characterization of exosomal microRNAs in preterm infants fed with breast milk and infant formula |
| title_full | Characterization of exosomal microRNAs in preterm infants fed with breast milk and infant formula |
| title_fullStr | Characterization of exosomal microRNAs in preterm infants fed with breast milk and infant formula |
| title_full_unstemmed | Characterization of exosomal microRNAs in preterm infants fed with breast milk and infant formula |
| title_short | Characterization of exosomal microRNAs in preterm infants fed with breast milk and infant formula |
| title_sort | characterization of exosomal micrornas in preterm infants fed with breast milk and infant formula |
| topic | preterm infant exosomes miRNA small RNA sequencing breastfeeding serum |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2024.1339919/full |
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