Rhizosphere Bacterial Communities Differ According to Fertilizer Regimes and Cabbage (Brassica oleracea var. capitata L.) Harvest Time, but Not Aphid Herbivory
Rhizosphere microbial communities are known to be highly diverse and strongly dependent on various attributes of the host plant, such as species, nutritional status, and growth stage. High-throughput 16S rRNA gene amplicon sequencing has been used to characterize the rhizosphere bacterial community...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-07-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.01620/full |
| _version_ | 1811314992077602816 |
|---|---|
| author | Flora J. M. O’Brien Flora J. M. O’Brien Marc G. Dumont Jeremy S. Webb Guy M. Poppy |
| author_facet | Flora J. M. O’Brien Flora J. M. O’Brien Marc G. Dumont Jeremy S. Webb Guy M. Poppy |
| author_sort | Flora J. M. O’Brien |
| collection | DOAJ |
| description | Rhizosphere microbial communities are known to be highly diverse and strongly dependent on various attributes of the host plant, such as species, nutritional status, and growth stage. High-throughput 16S rRNA gene amplicon sequencing has been used to characterize the rhizosphere bacterial community of many important crop species, but this is the first study to date to characterize the bacterial and archaeal community of Brassica oleracea var. capitata. The study also tested the response of the bacterial community to fertilizer type (organic or synthetic) and N dosage (high or low), in addition to plant age (9 or 12 weeks) and aphid (Myzus persicae) herbivory (present/absent). The impact of aboveground herbivory on belowground microbial communities has received little attention in the literature, and since the type (organic or mineral) and amount of fertilizer applications are known to affect M. percicae populations, these treatments were applied at agricultural rates to test for synergistic effects on the soil bacterial community. Fertilizer type and plant growth were found to result in significantly different rhizosphere bacterial communities, while there was no effect of aphid herbivory. Several operational taxonomic units were identified as varying significantly in abundance between the treatment groups and age cohorts. These included members of the S-oxidizing genus Thiobacillus, which was significantly more abundant in organically fertilized 12-week-old cabbages, and the N-fixing cyanobacteria Phormidium, which appeared to decline in synthetically fertilized soils relative to controls. These responses may be an effect of accumulating root-derived glucosinolates in the B. oleracea rhizosphere and increased N-availability, respectively. |
| first_indexed | 2024-04-13T11:22:24Z |
| format | Article |
| id | doaj.art-8ff9b4d60c3947ca9ccc8b40888bf2a6 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T11:22:24Z |
| publishDate | 2018-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-8ff9b4d60c3947ca9ccc8b40888bf2a62022-12-22T02:48:48ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-07-01910.3389/fmicb.2018.01620333756Rhizosphere Bacterial Communities Differ According to Fertilizer Regimes and Cabbage (Brassica oleracea var. capitata L.) Harvest Time, but Not Aphid HerbivoryFlora J. M. O’Brien0Flora J. M. O’Brien1Marc G. Dumont2Jeremy S. Webb3Guy M. Poppy4Biological Sciences, University of Southampton, Southampton, United KingdomNIAB EMR, East Malling, United KingdomBiological Sciences, University of Southampton, Southampton, United KingdomBiological Sciences, University of Southampton, Southampton, United KingdomBiological Sciences, University of Southampton, Southampton, United KingdomRhizosphere microbial communities are known to be highly diverse and strongly dependent on various attributes of the host plant, such as species, nutritional status, and growth stage. High-throughput 16S rRNA gene amplicon sequencing has been used to characterize the rhizosphere bacterial community of many important crop species, but this is the first study to date to characterize the bacterial and archaeal community of Brassica oleracea var. capitata. The study also tested the response of the bacterial community to fertilizer type (organic or synthetic) and N dosage (high or low), in addition to plant age (9 or 12 weeks) and aphid (Myzus persicae) herbivory (present/absent). The impact of aboveground herbivory on belowground microbial communities has received little attention in the literature, and since the type (organic or mineral) and amount of fertilizer applications are known to affect M. percicae populations, these treatments were applied at agricultural rates to test for synergistic effects on the soil bacterial community. Fertilizer type and plant growth were found to result in significantly different rhizosphere bacterial communities, while there was no effect of aphid herbivory. Several operational taxonomic units were identified as varying significantly in abundance between the treatment groups and age cohorts. These included members of the S-oxidizing genus Thiobacillus, which was significantly more abundant in organically fertilized 12-week-old cabbages, and the N-fixing cyanobacteria Phormidium, which appeared to decline in synthetically fertilized soils relative to controls. These responses may be an effect of accumulating root-derived glucosinolates in the B. oleracea rhizosphere and increased N-availability, respectively.https://www.frontiersin.org/article/10.3389/fmicb.2018.01620/fullrhizospherefertilizersplant growthbacterial communitiesBrassica oleracea |
| spellingShingle | Flora J. M. O’Brien Flora J. M. O’Brien Marc G. Dumont Jeremy S. Webb Guy M. Poppy Rhizosphere Bacterial Communities Differ According to Fertilizer Regimes and Cabbage (Brassica oleracea var. capitata L.) Harvest Time, but Not Aphid Herbivory Frontiers in Microbiology rhizosphere fertilizers plant growth bacterial communities Brassica oleracea |
| title | Rhizosphere Bacterial Communities Differ According to Fertilizer Regimes and Cabbage (Brassica oleracea var. capitata L.) Harvest Time, but Not Aphid Herbivory |
| title_full | Rhizosphere Bacterial Communities Differ According to Fertilizer Regimes and Cabbage (Brassica oleracea var. capitata L.) Harvest Time, but Not Aphid Herbivory |
| title_fullStr | Rhizosphere Bacterial Communities Differ According to Fertilizer Regimes and Cabbage (Brassica oleracea var. capitata L.) Harvest Time, but Not Aphid Herbivory |
| title_full_unstemmed | Rhizosphere Bacterial Communities Differ According to Fertilizer Regimes and Cabbage (Brassica oleracea var. capitata L.) Harvest Time, but Not Aphid Herbivory |
| title_short | Rhizosphere Bacterial Communities Differ According to Fertilizer Regimes and Cabbage (Brassica oleracea var. capitata L.) Harvest Time, but Not Aphid Herbivory |
| title_sort | rhizosphere bacterial communities differ according to fertilizer regimes and cabbage brassica oleracea var capitata l harvest time but not aphid herbivory |
| topic | rhizosphere fertilizers plant growth bacterial communities Brassica oleracea |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.01620/full |
| work_keys_str_mv | AT florajmobrien rhizospherebacterialcommunitiesdifferaccordingtofertilizerregimesandcabbagebrassicaoleraceavarcapitatalharvesttimebutnotaphidherbivory AT florajmobrien rhizospherebacterialcommunitiesdifferaccordingtofertilizerregimesandcabbagebrassicaoleraceavarcapitatalharvesttimebutnotaphidherbivory AT marcgdumont rhizospherebacterialcommunitiesdifferaccordingtofertilizerregimesandcabbagebrassicaoleraceavarcapitatalharvesttimebutnotaphidherbivory AT jeremyswebb rhizospherebacterialcommunitiesdifferaccordingtofertilizerregimesandcabbagebrassicaoleraceavarcapitatalharvesttimebutnotaphidherbivory AT guympoppy rhizospherebacterialcommunitiesdifferaccordingtofertilizerregimesandcabbagebrassicaoleraceavarcapitatalharvesttimebutnotaphidherbivory |