Glycation Leads to Increased Polysialylation and Promotes the Metastatic Potential of Neuroblastoma Cells
Neuroblastoma is the second most frequent extracranial tumor, affecting young children worldwide. One hallmark of tumors such as neuroblastomas, is the expression of polysialic acid, which interferes with adhesion and may promote invasion and metastasis. Since tumor cells use glycolysis for energy p...
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MDPI AG
2020-04-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/9/4/868 |
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| author | Maximilian Scheer Kaya Bork Frieder Simon Manimozhi Nagasundaram Rüdiger Horstkorte Vinayaga Srinivasan Gnanapragassam |
| author_facet | Maximilian Scheer Kaya Bork Frieder Simon Manimozhi Nagasundaram Rüdiger Horstkorte Vinayaga Srinivasan Gnanapragassam |
| author_sort | Maximilian Scheer |
| collection | DOAJ |
| description | Neuroblastoma is the second most frequent extracranial tumor, affecting young children worldwide. One hallmark of tumors such as neuroblastomas, is the expression of polysialic acid, which interferes with adhesion and may promote invasion and metastasis. Since tumor cells use glycolysis for energy production, they thereby produce as side product methylglyoxal (MGO), which reacts with proteins to advanced glycation end products in a mechanism called glycation. Here we analyzed the expression of (poly) sialic acid and adhesion of Kelly neuroblastoma cells after glycation with MGO. We found that both sialylation and polysialylation is increased after glycation. Furthermore, glycated Kelly neuroblastoma cells had a much higher potential for migration and invasion compared with non-glycated cells. |
| first_indexed | 2024-03-10T20:43:05Z |
| format | Article |
| id | doaj.art-8ffb589510d74a2e85168d1a4486f45b |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T20:43:05Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-8ffb589510d74a2e85168d1a4486f45b2023-11-19T20:33:09ZengMDPI AGCells2073-44092020-04-019486810.3390/cells9040868Glycation Leads to Increased Polysialylation and Promotes the Metastatic Potential of Neuroblastoma CellsMaximilian Scheer0Kaya Bork1Frieder Simon2Manimozhi Nagasundaram3Rüdiger Horstkorte4Vinayaga Srinivasan Gnanapragassam5Institute for Physiological Chemistry, Medical Faculty, Martin-Luther-University Halle-Wittenberg, 06114 Halle (Saale), GermanyInstitute for Physiological Chemistry, Medical Faculty, Martin-Luther-University Halle-Wittenberg, 06114 Halle (Saale), GermanyInstitute for Physiological Chemistry, Medical Faculty, Martin-Luther-University Halle-Wittenberg, 06114 Halle (Saale), GermanyInstitute for Physiological Chemistry, Medical Faculty, Martin-Luther-University Halle-Wittenberg, 06114 Halle (Saale), GermanyInstitute for Physiological Chemistry, Medical Faculty, Martin-Luther-University Halle-Wittenberg, 06114 Halle (Saale), GermanyInstitute for Physiological Chemistry, Medical Faculty, Martin-Luther-University Halle-Wittenberg, 06114 Halle (Saale), GermanyNeuroblastoma is the second most frequent extracranial tumor, affecting young children worldwide. One hallmark of tumors such as neuroblastomas, is the expression of polysialic acid, which interferes with adhesion and may promote invasion and metastasis. Since tumor cells use glycolysis for energy production, they thereby produce as side product methylglyoxal (MGO), which reacts with proteins to advanced glycation end products in a mechanism called glycation. Here we analyzed the expression of (poly) sialic acid and adhesion of Kelly neuroblastoma cells after glycation with MGO. We found that both sialylation and polysialylation is increased after glycation. Furthermore, glycated Kelly neuroblastoma cells had a much higher potential for migration and invasion compared with non-glycated cells.https://www.mdpi.com/2073-4409/9/4/868(poly) sialic acidNCAMadhesionmigrationmethylglyoxalKelly cells |
| spellingShingle | Maximilian Scheer Kaya Bork Frieder Simon Manimozhi Nagasundaram Rüdiger Horstkorte Vinayaga Srinivasan Gnanapragassam Glycation Leads to Increased Polysialylation and Promotes the Metastatic Potential of Neuroblastoma Cells Cells (poly) sialic acid NCAM adhesion migration methylglyoxal Kelly cells |
| title | Glycation Leads to Increased Polysialylation and Promotes the Metastatic Potential of Neuroblastoma Cells |
| title_full | Glycation Leads to Increased Polysialylation and Promotes the Metastatic Potential of Neuroblastoma Cells |
| title_fullStr | Glycation Leads to Increased Polysialylation and Promotes the Metastatic Potential of Neuroblastoma Cells |
| title_full_unstemmed | Glycation Leads to Increased Polysialylation and Promotes the Metastatic Potential of Neuroblastoma Cells |
| title_short | Glycation Leads to Increased Polysialylation and Promotes the Metastatic Potential of Neuroblastoma Cells |
| title_sort | glycation leads to increased polysialylation and promotes the metastatic potential of neuroblastoma cells |
| topic | (poly) sialic acid NCAM adhesion migration methylglyoxal Kelly cells |
| url | https://www.mdpi.com/2073-4409/9/4/868 |
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