Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (2): impacts of post-partum negative energy balance on stromal, glandular and luminal epithelial cells
Abstract Background In post-partum dairy cows, the energy needs to satisfy high milk production induces a status of more or less pronounced Negative Energy Balance (NEB). NEB associated with fat mobilization impairs reproductive function. In a companion paper, we described constitutive gene expressi...
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| Format: | Article |
| Language: | English |
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BMC
2021-06-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-021-07713-z |
| _version_ | 1819024664012259328 |
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| author | Wiruntita Chankeaw Sandra Lignier Christophe Richard Theodoros Ntallaris Mariam Raliou Yongzhi Guo Damien Plassard Claudia Bevilacqua Olivier Sandra Göran Andersson Patrice Humblot Gilles Charpigny |
| author_facet | Wiruntita Chankeaw Sandra Lignier Christophe Richard Theodoros Ntallaris Mariam Raliou Yongzhi Guo Damien Plassard Claudia Bevilacqua Olivier Sandra Göran Andersson Patrice Humblot Gilles Charpigny |
| author_sort | Wiruntita Chankeaw |
| collection | DOAJ |
| description | Abstract Background In post-partum dairy cows, the energy needs to satisfy high milk production induces a status of more or less pronounced Negative Energy Balance (NEB). NEB associated with fat mobilization impairs reproductive function. In a companion paper, we described constitutive gene expression in the three main endometrial cell types (stromal, glandular and luminal epithelial cells) isolated by laser capture micro-dissection (LCM) showing the specificities of their transcriptomic profiles. This study investigates the specific impact of NEB on gene expression in these cells around 80 days after parturition at day 15 of the oestrus cycle and describes their specific response to NEB. Results Following the description of their constitutive expression, the transcriptome profiles obtained by RNA sequencing of the three cells types revealed that differences related to the severity of NEB altered mainly specific patterns of expression related to individual cell types. Number of differentially expressed genes between severe NEB (SNEB) and mild NEB (MNEB) cows was higher in ST than in LE and GE, respectively. SNEB was associated with differential expression of genes coding for proteins involved in metabolic processes and embryo-maternal interactions in ST. Under-expression of genes encoding proteins with functions related to cell structure was found in GE whereas genes encoding proteins participating in pro-inflammatory pathways were over-expressed. Genes associated to adaptive immunity were under-expressed in LE. Conclusion The severity of NEB after calving is associated with changes in gene expression around 80 days after parturition corresponding to the time of breeding. Specific alterations in GEs are associated with activation of pro-inflammatory mechanisms. Concomitantly, changes in the expression of genes encoding proteins involved in cell interactions and maternal recognition of pregnancy takes place in ST. The combination of these effects possibly altering the uterine environment and embryo maternal interactions may negatively influence the establishment of pregnancy. |
| first_indexed | 2024-12-21T04:58:22Z |
| format | Article |
| id | doaj.art-8ffbe95d22704c1ab11dcd8c091d02e5 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-21T04:58:22Z |
| publishDate | 2021-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-8ffbe95d22704c1ab11dcd8c091d02e52022-12-21T19:15:18ZengBMCBMC Genomics1471-21642021-06-0122111810.1186/s12864-021-07713-zAnalysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (2): impacts of post-partum negative energy balance on stromal, glandular and luminal epithelial cellsWiruntita Chankeaw0Sandra Lignier1Christophe Richard2Theodoros Ntallaris3Mariam Raliou4Yongzhi Guo5Damien Plassard6Claudia Bevilacqua7Olivier Sandra8Göran Andersson9Patrice Humblot10Gilles Charpigny11Department of Clinical Sciences, Swedish University of Agricultural Sciences, SLUUniversité Paris-Saclay, UVSQ, INRAE, BREEDUniversité Paris-Saclay, UVSQ, INRAE, BREEDDepartment of Clinical Sciences, Swedish University of Agricultural Sciences, SLUUniversité Paris-Saclay, UVSQ, INRAE, BREEDDepartment of Clinical Sciences, Swedish University of Agricultural Sciences, SLUGenomEast Platform CERBM GIE, IGBMCUniversité Paris-Saclay, INRAE, AgroParisTech, GABIUniversité Paris-Saclay, UVSQ, INRAE, BREEDDepartment of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, SLUDepartment of Clinical Sciences, Swedish University of Agricultural Sciences, SLUUniversité Paris-Saclay, UVSQ, INRAE, BREEDAbstract Background In post-partum dairy cows, the energy needs to satisfy high milk production induces a status of more or less pronounced Negative Energy Balance (NEB). NEB associated with fat mobilization impairs reproductive function. In a companion paper, we described constitutive gene expression in the three main endometrial cell types (stromal, glandular and luminal epithelial cells) isolated by laser capture micro-dissection (LCM) showing the specificities of their transcriptomic profiles. This study investigates the specific impact of NEB on gene expression in these cells around 80 days after parturition at day 15 of the oestrus cycle and describes their specific response to NEB. Results Following the description of their constitutive expression, the transcriptome profiles obtained by RNA sequencing of the three cells types revealed that differences related to the severity of NEB altered mainly specific patterns of expression related to individual cell types. Number of differentially expressed genes between severe NEB (SNEB) and mild NEB (MNEB) cows was higher in ST than in LE and GE, respectively. SNEB was associated with differential expression of genes coding for proteins involved in metabolic processes and embryo-maternal interactions in ST. Under-expression of genes encoding proteins with functions related to cell structure was found in GE whereas genes encoding proteins participating in pro-inflammatory pathways were over-expressed. Genes associated to adaptive immunity were under-expressed in LE. Conclusion The severity of NEB after calving is associated with changes in gene expression around 80 days after parturition corresponding to the time of breeding. Specific alterations in GEs are associated with activation of pro-inflammatory mechanisms. Concomitantly, changes in the expression of genes encoding proteins involved in cell interactions and maternal recognition of pregnancy takes place in ST. The combination of these effects possibly altering the uterine environment and embryo maternal interactions may negatively influence the establishment of pregnancy.https://doi.org/10.1186/s12864-021-07713-zNegative energy balanceEndometriumCell type-specificTranscriptomeLCMRNA-seq |
| spellingShingle | Wiruntita Chankeaw Sandra Lignier Christophe Richard Theodoros Ntallaris Mariam Raliou Yongzhi Guo Damien Plassard Claudia Bevilacqua Olivier Sandra Göran Andersson Patrice Humblot Gilles Charpigny Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (2): impacts of post-partum negative energy balance on stromal, glandular and luminal epithelial cells BMC Genomics Negative energy balance Endometrium Cell type-specific Transcriptome LCM RNA-seq |
| title | Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (2): impacts of post-partum negative energy balance on stromal, glandular and luminal epithelial cells |
| title_full | Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (2): impacts of post-partum negative energy balance on stromal, glandular and luminal epithelial cells |
| title_fullStr | Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (2): impacts of post-partum negative energy balance on stromal, glandular and luminal epithelial cells |
| title_full_unstemmed | Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (2): impacts of post-partum negative energy balance on stromal, glandular and luminal epithelial cells |
| title_short | Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (2): impacts of post-partum negative energy balance on stromal, glandular and luminal epithelial cells |
| title_sort | analysis of the transcriptome of bovine endometrial cells isolated by laser micro dissection 2 impacts of post partum negative energy balance on stromal glandular and luminal epithelial cells |
| topic | Negative energy balance Endometrium Cell type-specific Transcriptome LCM RNA-seq |
| url | https://doi.org/10.1186/s12864-021-07713-z |
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