STUDY ON THE SEPARATION THROUGH THIN LAYER CHROMATOGRAPHY OF THE CHOLESTEROL IN THE BUFFALO MILK ORIGINATING IN THE MESENDORF FARM
Within the present study, we conducted a thin layer chromatographic separation of neuter fats in buffalo milk, following sodium metoxyde saponification and the exposition to λ=366 nm. The extraction of free and esterized milk cholesterol was conducted according to the Folch et al. (1957) method. The...
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-11-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | https://spasb.ro/index.php/public_html/article/view/2065 |
| _version_ | 1797223801424969728 |
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| author | AURELIA PECE C. COROIAN BIANCA GHIRILĂ G. MUREŞAN |
| author_facet | AURELIA PECE C. COROIAN BIANCA GHIRILĂ G. MUREŞAN |
| author_sort | AURELIA PECE |
| collection | DOAJ |
| description | Within the present study, we conducted a thin layer chromatographic separation of
neuter fats in buffalo milk, following sodium metoxyde saponification and the
exposition to λ=366 nm. The extraction of free and esterized milk cholesterol was
conducted according to the Folch et al. (1957) method. The free and esterized
cholesterol, as well as triglycerids appear as brown spots (iodine complexes with the
double connections between the compounds under anlysis). Qualitative identification
was conducted through the comparison of Rfs for free and esterized cholesterol, in
the samples analyzed with existing standards. The semi-quantitative anlysis was
conducted through a visual comparison of the intensity encountered in free
cholesterol spots within samples under anlysis and the standard of different
concentrations applied on the same board. Buffalo milk samples originated in the
Mesendorf Farm, considered and certified as an ecologic farm. |
| first_indexed | 2024-04-24T13:43:00Z |
| format | Article |
| id | doaj.art-8fffedeed8fb41bd8825c061eaffb91c |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T13:43:00Z |
| publishDate | 2023-11-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-8fffedeed8fb41bd8825c061eaffb91c2024-04-04T07:37:03ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-11-014122362362065STUDY ON THE SEPARATION THROUGH THIN LAYER CHROMATOGRAPHY OF THE CHOLESTEROL IN THE BUFFALO MILK ORIGINATING IN THE MESENDORF FARMAURELIA PECE0C. COROIAN1BIANCA GHIRILĂ2G. MUREŞAN3University Agricultural Science and Veterinary Medicine, Faculty of Animal Husbandry and Biotehnology, 3 – 5 Mănăştur Street, Cluj-Napoca, RomaniaUniversity Agricultural Science and Veterinary Medicine, Faculty of Animal Husbandry and Biotehnology, 3 – 5 Mănăştur Street, Cluj-Napoca, RomaniaUniversity Agricultural Science and Veterinary Medicine, Faculty of Animal Husbandry and Biotehnology, 3 – 5 Mănăştur Street, Cluj-Napoca, Romania University Agricultural Science and Veterinary Medicine, Faculty of Animal Husbandry and Biotehnology, 3 – 5 Mănăştur Street, Cluj-Napoca, Romania Within the present study, we conducted a thin layer chromatographic separation of neuter fats in buffalo milk, following sodium metoxyde saponification and the exposition to λ=366 nm. The extraction of free and esterized milk cholesterol was conducted according to the Folch et al. (1957) method. The free and esterized cholesterol, as well as triglycerids appear as brown spots (iodine complexes with the double connections between the compounds under anlysis). Qualitative identification was conducted through the comparison of Rfs for free and esterized cholesterol, in the samples analyzed with existing standards. The semi-quantitative anlysis was conducted through a visual comparison of the intensity encountered in free cholesterol spots within samples under anlysis and the standard of different concentrations applied on the same board. Buffalo milk samples originated in the Mesendorf Farm, considered and certified as an ecologic farm.https://spasb.ro/index.php/public_html/article/view/2065cholesterolmilkbuffalo |
| spellingShingle | AURELIA PECE C. COROIAN BIANCA GHIRILĂ G. MUREŞAN STUDY ON THE SEPARATION THROUGH THIN LAYER CHROMATOGRAPHY OF THE CHOLESTEROL IN THE BUFFALO MILK ORIGINATING IN THE MESENDORF FARM Scientific Papers Animal Science and Biotechnologies cholesterol milk buffalo |
| title | STUDY ON THE SEPARATION THROUGH THIN LAYER CHROMATOGRAPHY OF THE CHOLESTEROL IN THE BUFFALO MILK ORIGINATING IN THE MESENDORF FARM |
| title_full | STUDY ON THE SEPARATION THROUGH THIN LAYER CHROMATOGRAPHY OF THE CHOLESTEROL IN THE BUFFALO MILK ORIGINATING IN THE MESENDORF FARM |
| title_fullStr | STUDY ON THE SEPARATION THROUGH THIN LAYER CHROMATOGRAPHY OF THE CHOLESTEROL IN THE BUFFALO MILK ORIGINATING IN THE MESENDORF FARM |
| title_full_unstemmed | STUDY ON THE SEPARATION THROUGH THIN LAYER CHROMATOGRAPHY OF THE CHOLESTEROL IN THE BUFFALO MILK ORIGINATING IN THE MESENDORF FARM |
| title_short | STUDY ON THE SEPARATION THROUGH THIN LAYER CHROMATOGRAPHY OF THE CHOLESTEROL IN THE BUFFALO MILK ORIGINATING IN THE MESENDORF FARM |
| title_sort | study on the separation through thin layer chromatography of the cholesterol in the buffalo milk originating in the mesendorf farm |
| topic | cholesterol milk buffalo |
| url | https://spasb.ro/index.php/public_html/article/view/2065 |
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