Screening of Spore-Forming Bacteria with Probiotic Potential in Pristine Algerian Caves
ABSTRACT The interest and exploration of biodiversity in subsurface ecosystems have increased significantly during the last 2 decades. The aim of this study was to investigate the in vitro probiotic properties of spore-forming bacteria isolated from deep caves. Two hundred fifty spore-forming microb...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2022-10-01
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| Series: | Microbiology Spectrum |
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.00248-22 |
| _version_ | 1811329712958472192 |
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| author | Baraa Rehamnia Natuschka M. Lee Ramune Kuktaite Noreddine Kacem Chaouche |
| author_facet | Baraa Rehamnia Natuschka M. Lee Ramune Kuktaite Noreddine Kacem Chaouche |
| author_sort | Baraa Rehamnia |
| collection | DOAJ |
| description | ABSTRACT The interest and exploration of biodiversity in subsurface ecosystems have increased significantly during the last 2 decades. The aim of this study was to investigate the in vitro probiotic properties of spore-forming bacteria isolated from deep caves. Two hundred fifty spore-forming microbes were enriched from sediment samples from 10 different pristine caves in Algeria at different depths. Isolates showing nonpathogenic profiles were screened for their potential to produce digestive enzymes (gliadinase and beta-galactosidase) in solid and liquid media, respectively. Different probiotic potentialities were studied, including (i) growth at 37°C, (ii) survival in simulated gastric juice, (iii) survival in simulated intestinal fluid, and (iv) antibiotic sensitivity and cell surface properties. The results showed that out of 250 isolates, 13 isolates demonstrated nonpathogenic character, probiotic potentialities, and ability to hydrolyze gliadin and lactose in solution. These findings suggest that a selection of cave microbes might serve as a source of interesting candidates for probiotics. IMPORTANCE Previous microbial studies of subsurface ecosystems like caves focused mainly on the natural biodiversity in these systems. So far, only a few studies focused on the biotechnological potential of microbes in these systems, focusing in particular on their antibacterial potential, antibiotic production, and, to some extent, enzymatic potential. This study explores whether subsurface ecosystems can serve as an alternative source for microbes relevant to probiotics. The research focused on the ability of cave microbes to degrade two substrates (lactose and gliadin) that cause common digestive disorders. Since these enzymes may prove to be useful in food processing and in reducing the effect of lactose and gliadin digestion within intolerant patients, isolation of microbes such as in this study may expand the possibilities of developing alternative strategies to deal with these intolerances. |
| first_indexed | 2024-04-13T15:48:44Z |
| format | Article |
| id | doaj.art-9002e2fffdd544de8e8e6e433043e36a |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-04-13T15:48:44Z |
| publishDate | 2022-10-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-9002e2fffdd544de8e8e6e433043e36a2022-12-22T02:40:54ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972022-10-0110510.1128/spectrum.00248-22Screening of Spore-Forming Bacteria with Probiotic Potential in Pristine Algerian CavesBaraa Rehamnia0Natuschka M. Lee1Ramune Kuktaite2Noreddine Kacem Chaouche3Laboratoire de Mycologie, de Biotechnologie et l’Activité Microbienne (LaMyBAM), Université Frères Mentouri Constantine 1, Constantine, AlgeriaMicrobial Geoecology and Astrobiology, Department of Ecology and Environmental Science, Umeå University, Umeå, SwedenDepartment of Plant Breeding, Swedish University of Agricultural Sciences (Alnarp), Lomma, SwedenLaboratoire de Mycologie, de Biotechnologie et l’Activité Microbienne (LaMyBAM), Université Frères Mentouri Constantine 1, Constantine, AlgeriaABSTRACT The interest and exploration of biodiversity in subsurface ecosystems have increased significantly during the last 2 decades. The aim of this study was to investigate the in vitro probiotic properties of spore-forming bacteria isolated from deep caves. Two hundred fifty spore-forming microbes were enriched from sediment samples from 10 different pristine caves in Algeria at different depths. Isolates showing nonpathogenic profiles were screened for their potential to produce digestive enzymes (gliadinase and beta-galactosidase) in solid and liquid media, respectively. Different probiotic potentialities were studied, including (i) growth at 37°C, (ii) survival in simulated gastric juice, (iii) survival in simulated intestinal fluid, and (iv) antibiotic sensitivity and cell surface properties. The results showed that out of 250 isolates, 13 isolates demonstrated nonpathogenic character, probiotic potentialities, and ability to hydrolyze gliadin and lactose in solution. These findings suggest that a selection of cave microbes might serve as a source of interesting candidates for probiotics. IMPORTANCE Previous microbial studies of subsurface ecosystems like caves focused mainly on the natural biodiversity in these systems. So far, only a few studies focused on the biotechnological potential of microbes in these systems, focusing in particular on their antibacterial potential, antibiotic production, and, to some extent, enzymatic potential. This study explores whether subsurface ecosystems can serve as an alternative source for microbes relevant to probiotics. The research focused on the ability of cave microbes to degrade two substrates (lactose and gliadin) that cause common digestive disorders. Since these enzymes may prove to be useful in food processing and in reducing the effect of lactose and gliadin digestion within intolerant patients, isolation of microbes such as in this study may expand the possibilities of developing alternative strategies to deal with these intolerances.https://journals.asm.org/doi/10.1128/spectrum.00248-22subsurfacecave geobiologygliadinasebeta-galactosidaselactoseprobiotics |
| spellingShingle | Baraa Rehamnia Natuschka M. Lee Ramune Kuktaite Noreddine Kacem Chaouche Screening of Spore-Forming Bacteria with Probiotic Potential in Pristine Algerian Caves Microbiology Spectrum subsurface cave geobiology gliadinase beta-galactosidase lactose probiotics |
| title | Screening of Spore-Forming Bacteria with Probiotic Potential in Pristine Algerian Caves |
| title_full | Screening of Spore-Forming Bacteria with Probiotic Potential in Pristine Algerian Caves |
| title_fullStr | Screening of Spore-Forming Bacteria with Probiotic Potential in Pristine Algerian Caves |
| title_full_unstemmed | Screening of Spore-Forming Bacteria with Probiotic Potential in Pristine Algerian Caves |
| title_short | Screening of Spore-Forming Bacteria with Probiotic Potential in Pristine Algerian Caves |
| title_sort | screening of spore forming bacteria with probiotic potential in pristine algerian caves |
| topic | subsurface cave geobiology gliadinase beta-galactosidase lactose probiotics |
| url | https://journals.asm.org/doi/10.1128/spectrum.00248-22 |
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