Three Component Composite Scaffolds Based on PCL, Hydroxyapatite, and L-Lysine Obtained in TIPS-SL: Bioactive Material for Bone Tissue Engineering
In this research, we describe the properties of three-component composite foam scaffolds based on poly(ε-caprolactone) (PCL) as a matrix and hydroxyapatite whiskers (HAP) and L-Lysine as fillers (PCL/HAP/Lys with wt% ratio 50/48/2). The scaffolds were prepared using a thermally induced phase separat...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/24/13589 |
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| author | Aleksandra Korbut Marcin Włodarczyk Karolina Rudnicka Aleksandra Szwed Przemysław Płociński Monika Biernat Paulina Tymowicz-Grzyb Martyna Michalska Natalia Karska Sylwia Rodziewicz-Motowidło Konrad Szustakiewicz |
| author_facet | Aleksandra Korbut Marcin Włodarczyk Karolina Rudnicka Aleksandra Szwed Przemysław Płociński Monika Biernat Paulina Tymowicz-Grzyb Martyna Michalska Natalia Karska Sylwia Rodziewicz-Motowidło Konrad Szustakiewicz |
| author_sort | Aleksandra Korbut |
| collection | DOAJ |
| description | In this research, we describe the properties of three-component composite foam scaffolds based on poly(ε-caprolactone) (PCL) as a matrix and hydroxyapatite whiskers (HAP) and L-Lysine as fillers (PCL/HAP/Lys with wt% ratio 50/48/2). The scaffolds were prepared using a thermally induced phase separation technique supported by salt leaching (TIPS-SL). All materials were precisely characterized: porosity, density, water uptake, wettability, DSC, and TGA measurements and compression tests were carried out. The microstructure of the obtained scaffolds was analyzed via SEM. It was found that the PCL/HAP/Lys scaffold has a 45% higher Young’s modulus and better wettability compared to the PCL/HAP system. At the same time, the porosity of the system was ~90%. The osteoblast hFOB 1.19 cell response was also investigated in osteogenic conditions (39 °C) and the cytokine release profile of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α was determined. Modification of PCL scaffolds with HAP and L-Lysine significantly improved the proliferation of pre-osteoblasts cultured on such materials. |
| first_indexed | 2024-03-10T03:54:46Z |
| format | Article |
| id | doaj.art-9004bb9c67264d85a88867445533ea0e |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:54:46Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9004bb9c67264d85a88867445533ea0e2023-11-23T08:48:40ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-12-0122241358910.3390/ijms222413589Three Component Composite Scaffolds Based on PCL, Hydroxyapatite, and L-Lysine Obtained in TIPS-SL: Bioactive Material for Bone Tissue EngineeringAleksandra Korbut0Marcin Włodarczyk1Karolina Rudnicka2Aleksandra Szwed3Przemysław Płociński4Monika Biernat5Paulina Tymowicz-Grzyb6Martyna Michalska7Natalia Karska8Sylwia Rodziewicz-Motowidło9Konrad Szustakiewicz10Department of Polymer Engineering and Technology, Faculty of Chemistry, Wrocław University of Science and Technology (WUST), Wyb. Wyspiańskiego 27, 50-370 Wrocław, PolandDepartment of Immunology and Infectious Biology, Faculty of Biology and Environmental Protection, University of Łódź, Banacha 12/16, 90-237 Łódź, PolandDepartment of Immunology and Infectious Biology, Faculty of Biology and Environmental Protection, University of Łódź, Banacha 12/16, 90-237 Łódź, PolandDepartment of Immunology and Infectious Biology, Faculty of Biology and Environmental Protection, University of Łódź, Banacha 12/16, 90-237 Łódź, PolandDepartment of Immunology and Infectious Biology, Faculty of Biology and Environmental Protection, University of Łódź, Banacha 12/16, 90-237 Łódź, PolandBiomaterials Research Group, Ceramic and Concrete Division in Warsaw, Łukasiewicz Research Network Institute of Ceramics and Building Materials, Postępu 9, 02-676 Warsaw, PolandBiomaterials Research Group, Ceramic and Concrete Division in Warsaw, Łukasiewicz Research Network Institute of Ceramics and Building Materials, Postępu 9, 02-676 Warsaw, PolandDepartment of Polymer Engineering and Technology, Faculty of Chemistry, Wrocław University of Science and Technology (WUST), Wyb. Wyspiańskiego 27, 50-370 Wrocław, PolandFaculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308 Gdańsk, PolandFaculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308 Gdańsk, PolandDepartment of Polymer Engineering and Technology, Faculty of Chemistry, Wrocław University of Science and Technology (WUST), Wyb. Wyspiańskiego 27, 50-370 Wrocław, PolandIn this research, we describe the properties of three-component composite foam scaffolds based on poly(ε-caprolactone) (PCL) as a matrix and hydroxyapatite whiskers (HAP) and L-Lysine as fillers (PCL/HAP/Lys with wt% ratio 50/48/2). The scaffolds were prepared using a thermally induced phase separation technique supported by salt leaching (TIPS-SL). All materials were precisely characterized: porosity, density, water uptake, wettability, DSC, and TGA measurements and compression tests were carried out. The microstructure of the obtained scaffolds was analyzed via SEM. It was found that the PCL/HAP/Lys scaffold has a 45% higher Young’s modulus and better wettability compared to the PCL/HAP system. At the same time, the porosity of the system was ~90%. The osteoblast hFOB 1.19 cell response was also investigated in osteogenic conditions (39 °C) and the cytokine release profile of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α was determined. Modification of PCL scaffolds with HAP and L-Lysine significantly improved the proliferation of pre-osteoblasts cultured on such materials.https://www.mdpi.com/1422-0067/22/24/13589polymersfoam scaffoldsPCLcell adhesionL-Lysine |
| spellingShingle | Aleksandra Korbut Marcin Włodarczyk Karolina Rudnicka Aleksandra Szwed Przemysław Płociński Monika Biernat Paulina Tymowicz-Grzyb Martyna Michalska Natalia Karska Sylwia Rodziewicz-Motowidło Konrad Szustakiewicz Three Component Composite Scaffolds Based on PCL, Hydroxyapatite, and L-Lysine Obtained in TIPS-SL: Bioactive Material for Bone Tissue Engineering International Journal of Molecular Sciences polymers foam scaffolds PCL cell adhesion L-Lysine |
| title | Three Component Composite Scaffolds Based on PCL, Hydroxyapatite, and L-Lysine Obtained in TIPS-SL: Bioactive Material for Bone Tissue Engineering |
| title_full | Three Component Composite Scaffolds Based on PCL, Hydroxyapatite, and L-Lysine Obtained in TIPS-SL: Bioactive Material for Bone Tissue Engineering |
| title_fullStr | Three Component Composite Scaffolds Based on PCL, Hydroxyapatite, and L-Lysine Obtained in TIPS-SL: Bioactive Material for Bone Tissue Engineering |
| title_full_unstemmed | Three Component Composite Scaffolds Based on PCL, Hydroxyapatite, and L-Lysine Obtained in TIPS-SL: Bioactive Material for Bone Tissue Engineering |
| title_short | Three Component Composite Scaffolds Based on PCL, Hydroxyapatite, and L-Lysine Obtained in TIPS-SL: Bioactive Material for Bone Tissue Engineering |
| title_sort | three component composite scaffolds based on pcl hydroxyapatite and l lysine obtained in tips sl bioactive material for bone tissue engineering |
| topic | polymers foam scaffolds PCL cell adhesion L-Lysine |
| url | https://www.mdpi.com/1422-0067/22/24/13589 |
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