Antimicrobial and Antioxidant Activity of Natural Honeys of Different Origin
To examine the antimicrobial and antioxidant activity of 15 natural honeys, honey samples were collected from different locations of Slovakia, Poland and Serbia. For antimicrobial activity determination honey solutions were prepared at three concentrations: 50, 25 and 12.5 % (by mass per volume). Th...
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-09-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
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| Online Access: | https://spasb.ro/index.php/public_html/article/view/192 |
| _version_ | 1797214767159443456 |
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| author | Martina Fikselová Miroslava Kačániová Lukáš Hleba Martin Mellen Nenad Vuković Malgorzata Džugan |
| author_facet | Martina Fikselová Miroslava Kačániová Lukáš Hleba Martin Mellen Nenad Vuković Malgorzata Džugan |
| author_sort | Martina Fikselová |
| collection | DOAJ |
| description | To examine the antimicrobial and antioxidant activity of 15 natural honeys, honey samples were collected from different locations of Slovakia, Poland and Serbia. For antimicrobial activity determination honey solutions were prepared at three concentrations: 50, 25 and 12.5 % (by mass per volume). The potential antimicrobial activity of selected samples against four species of bacteria (Escherichia coli CCM 3988, Pseudomonas aeroginosa CCM 1960, Staphylococcus epidermis CCM 4418, Bacillus cereus CCM 2010) and two species of yeasts (Saccharomyces cerevisiae CCM 8191, Candida albicans CCM 8216) was studied using the disc diffusion method. After incubation, the zones of inhibition of the growth of the microorganisms around the disks were measured. The strongest antimicrobial activity was shown at honey samples of 50 % concentration against Escherichia coli, Pseudomonas aeroginosa and Staphylococcus epidermis. Against Saccharomyces cerevisae and Candida albicans very low (at 50 %, 25 % concentration) or zero antifugal (at 12.5 % concentration) activity was determined. From the results obtained it was shown the variable ability of honey samples to scavenge stable free radical DPPH. TEACDPPH values ranged between 0.1-1.0 mmol.kg-1. As the antioxidative best source buckwheat honey was manifested and the lowest antioxidant activity was shown at acacia honey. |
| first_indexed | 2024-04-24T11:19:24Z |
| format | Article |
| id | doaj.art-900ab2e2bead4c0893d22a76298819b6 |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:19:24Z |
| publishDate | 2023-09-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-900ab2e2bead4c0893d22a76298819b62024-04-11T05:05:15ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-09-01472218218192Antimicrobial and Antioxidant Activity of Natural Honeys of Different OriginMartina Fikselová0Miroslava Kačániová1Lukáš Hleba2Martin Mellen3Nenad Vuković4Malgorzata Džugan5Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra , Tr. A. Hlinku 2, 949 76, SlovakiaDepartment of Microbiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Tr. A. Hlinku 2, 949 76, SlovakiaDepartment of Microbiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Tr. A. Hlinku 2, 949 76, SlovakiaDepartment of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra , Tr. A. Hlinku 2, 949 76, SlovakiaDepartment of Chemistry, Faculty of Science, University of Kragujevac, Kragujevac, Radoja Domanovica 12, 34000, SerbiaDepartment of Food Chemistry and Toxicology, Faculty of Biology and Agriculture, University of Rzeszow, Rzeszow 2, Ćwiklińskiej St., 35-601 PolandTo examine the antimicrobial and antioxidant activity of 15 natural honeys, honey samples were collected from different locations of Slovakia, Poland and Serbia. For antimicrobial activity determination honey solutions were prepared at three concentrations: 50, 25 and 12.5 % (by mass per volume). The potential antimicrobial activity of selected samples against four species of bacteria (Escherichia coli CCM 3988, Pseudomonas aeroginosa CCM 1960, Staphylococcus epidermis CCM 4418, Bacillus cereus CCM 2010) and two species of yeasts (Saccharomyces cerevisiae CCM 8191, Candida albicans CCM 8216) was studied using the disc diffusion method. After incubation, the zones of inhibition of the growth of the microorganisms around the disks were measured. The strongest antimicrobial activity was shown at honey samples of 50 % concentration against Escherichia coli, Pseudomonas aeroginosa and Staphylococcus epidermis. Against Saccharomyces cerevisae and Candida albicans very low (at 50 %, 25 % concentration) or zero antifugal (at 12.5 % concentration) activity was determined. From the results obtained it was shown the variable ability of honey samples to scavenge stable free radical DPPH. TEACDPPH values ranged between 0.1-1.0 mmol.kg-1. As the antioxidative best source buckwheat honey was manifested and the lowest antioxidant activity was shown at acacia honey.https://spasb.ro/index.php/public_html/article/view/192antimicrobial activityantioxidantsbacteriadpphnatural honeyyeasts |
| spellingShingle | Martina Fikselová Miroslava Kačániová Lukáš Hleba Martin Mellen Nenad Vuković Malgorzata Džugan Antimicrobial and Antioxidant Activity of Natural Honeys of Different Origin Scientific Papers Animal Science and Biotechnologies antimicrobial activity antioxidants bacteria dpph natural honey yeasts |
| title | Antimicrobial and Antioxidant Activity of Natural Honeys of Different Origin |
| title_full | Antimicrobial and Antioxidant Activity of Natural Honeys of Different Origin |
| title_fullStr | Antimicrobial and Antioxidant Activity of Natural Honeys of Different Origin |
| title_full_unstemmed | Antimicrobial and Antioxidant Activity of Natural Honeys of Different Origin |
| title_short | Antimicrobial and Antioxidant Activity of Natural Honeys of Different Origin |
| title_sort | antimicrobial and antioxidant activity of natural honeys of different origin |
| topic | antimicrobial activity antioxidants bacteria dpph natural honey yeasts |
| url | https://spasb.ro/index.php/public_html/article/view/192 |
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