Volatile Profile of Portuguese Monofloral Honeys: Significance in Botanical Origin Determination
The volatile profiles of 51 samples from 12 monofloral-labelled Portuguese honey types were assessed. Honeys of bell heather, carob tree, chestnut, eucalyptus, incense, lavender, orange, rape, raspberry, rosemary, sunflower and strawberry tree were collected from several regions from mainland Portug...
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MDPI AG
2021-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/16/4970 |
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| author | Alexandra M. Machado Marília Antunes Maria Graça Miguel Miguel Vilas-Boas Ana Cristina Figueiredo |
| author_facet | Alexandra M. Machado Marília Antunes Maria Graça Miguel Miguel Vilas-Boas Ana Cristina Figueiredo |
| author_sort | Alexandra M. Machado |
| collection | DOAJ |
| description | The volatile profiles of 51 samples from 12 monofloral-labelled Portuguese honey types were assessed. Honeys of bell heather, carob tree, chestnut, eucalyptus, incense, lavender, orange, rape, raspberry, rosemary, sunflower and strawberry tree were collected from several regions from mainland Portugal and from the Azores Islands. When available, the corresponding flower volatiles were comparatively evaluated. Honey volatiles were isolated using two different extraction methods, solid-phase microextraction (SPME) and hydrodistillation (HD), with HD proving to be more effective in the number of volatiles extracted. Agglomerative cluster analysis of honey HD volatiles evidenced two main clusters, one of which had nine sub-clusters. Components grouped by biosynthetic pathway defined alkanes and fatty acids as dominant, namely <i>n</i>-nonadecane, <i>n</i>-heneicosane, <i>n</i>-tricosane and <i>n</i>-pentacosane and palmitic, linoleic and oleic acids. Oxygen-containing monoterpenes, such as <i>cis</i>- and <i>trans</i>-linalool oxide (furanoid), hotrienol and the apocarotenoid α-isophorone, were also present in lower amounts. Aromatic amino acid derivatives were also identified, namely benzene acetaldehyde and 3,4,5-trimethylphenol. Fully grown classification tree analysis allowed the identification of the most relevant volatiles for discriminating the different honey types. Twelve volatile compounds were enough to fully discriminate eleven honey types (92%) according to the botanical origin. |
| first_indexed | 2024-03-10T08:33:20Z |
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| language | English |
| last_indexed | 2024-03-10T08:33:20Z |
| publishDate | 2021-08-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-900bc8f7a0d346f2b1be2215620470342023-11-22T08:54:40ZengMDPI AGMolecules1420-30492021-08-012616497010.3390/molecules26164970Volatile Profile of Portuguese Monofloral Honeys: Significance in Botanical Origin DeterminationAlexandra M. Machado0Marília Antunes1Maria Graça Miguel2Miguel Vilas-Boas3Ana Cristina Figueiredo4Centro de Estudos do Ambiente e do Mar (CESAM Lisboa), Centro de Biotecnologia Vegetal (CBV), Faculdade de Ciências da Universidade de Lisboa, DBV, C2, Piso 1, Campo Grande, 1749-016 Lisboa, PortugalCentro de Estatística e Aplicações (CEAUL), Departamento de Estatística e Investigação Operacional, Faculdade de Ciências da Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, PortugalFaculdade de Ciências e Tecnologia, Mediterranean Institute for Agriculture, Environment and Development, Campus de Gambelas, Universidade do Algarve, 8005-139 Faro, PortugalCIMO, Centro de Investigação de Montanha, Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, PortugalCentro de Estudos do Ambiente e do Mar (CESAM Lisboa), Centro de Biotecnologia Vegetal (CBV), Faculdade de Ciências da Universidade de Lisboa, DBV, C2, Piso 1, Campo Grande, 1749-016 Lisboa, PortugalThe volatile profiles of 51 samples from 12 monofloral-labelled Portuguese honey types were assessed. Honeys of bell heather, carob tree, chestnut, eucalyptus, incense, lavender, orange, rape, raspberry, rosemary, sunflower and strawberry tree were collected from several regions from mainland Portugal and from the Azores Islands. When available, the corresponding flower volatiles were comparatively evaluated. Honey volatiles were isolated using two different extraction methods, solid-phase microextraction (SPME) and hydrodistillation (HD), with HD proving to be more effective in the number of volatiles extracted. Agglomerative cluster analysis of honey HD volatiles evidenced two main clusters, one of which had nine sub-clusters. Components grouped by biosynthetic pathway defined alkanes and fatty acids as dominant, namely <i>n</i>-nonadecane, <i>n</i>-heneicosane, <i>n</i>-tricosane and <i>n</i>-pentacosane and palmitic, linoleic and oleic acids. Oxygen-containing monoterpenes, such as <i>cis</i>- and <i>trans</i>-linalool oxide (furanoid), hotrienol and the apocarotenoid α-isophorone, were also present in lower amounts. Aromatic amino acid derivatives were also identified, namely benzene acetaldehyde and 3,4,5-trimethylphenol. Fully grown classification tree analysis allowed the identification of the most relevant volatiles for discriminating the different honey types. Twelve volatile compounds were enough to fully discriminate eleven honey types (92%) according to the botanical origin.https://www.mdpi.com/1420-3049/26/16/4970Portuguese honeysvolatile profilehoney type discriminationbotanical origin determination |
| spellingShingle | Alexandra M. Machado Marília Antunes Maria Graça Miguel Miguel Vilas-Boas Ana Cristina Figueiredo Volatile Profile of Portuguese Monofloral Honeys: Significance in Botanical Origin Determination Molecules Portuguese honeys volatile profile honey type discrimination botanical origin determination |
| title | Volatile Profile of Portuguese Monofloral Honeys: Significance in Botanical Origin Determination |
| title_full | Volatile Profile of Portuguese Monofloral Honeys: Significance in Botanical Origin Determination |
| title_fullStr | Volatile Profile of Portuguese Monofloral Honeys: Significance in Botanical Origin Determination |
| title_full_unstemmed | Volatile Profile of Portuguese Monofloral Honeys: Significance in Botanical Origin Determination |
| title_short | Volatile Profile of Portuguese Monofloral Honeys: Significance in Botanical Origin Determination |
| title_sort | volatile profile of portuguese monofloral honeys significance in botanical origin determination |
| topic | Portuguese honeys volatile profile honey type discrimination botanical origin determination |
| url | https://www.mdpi.com/1420-3049/26/16/4970 |
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