Effect of Lactobacillus pentosus-Fermented Artemisiae Argi Folium on Nitric Oxide Production of Macrophage impaired with Various Toxicants
Objectives : The purpose of this study is to investigate the effect of Water Extract from Lactobacillus pentosus-fermented ARTEMISIAE ARGI FOLIUM (AFL) on nitric oxide production of mouse macrophage Raw 264.7 cells impaired by various toxicants such as gallic acid, EtOH, nicotine, acetaminophen, and...
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| Format: | Article |
| Language: | English |
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Korean Pharmacopuncture Institute
2009-12-01
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| Series: | Journal of Pharmacopuncture |
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| Online Access: | http://dx.doi.org/10.3831/KPI.2009.12.4.089 |
| _version_ | 1819171763843497984 |
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| author | Wansu Park |
| author_facet | Wansu Park |
| author_sort | Wansu Park |
| collection | DOAJ |
| description | Objectives : The purpose of this study is to investigate the effect of Water Extract from Lactobacillus pentosus-fermented ARTEMISIAE ARGI FOLIUM (AFL) on nitric oxide production of mouse macrophage Raw 264.7 cells impaired by various toxicants such as gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde.
Methods : ARTEMISIAE ARGI FOLIUM was fermented with Lactobacillus pentosus and extracted by water. Nitric oxide production of mouse macrophage Raw 264.7 cells was measured by Griess reagent assay. Examined concentrations of AFL were 10, 50, 100, 200, 400 ug/mL.
Results : The results of the experiment are as below.
1. AFL at the concentration of 400 ug/mL significantly recovered nitric oxide production which was reduced by gallic acid (100 uM) in Raw 264.7 cells.
2. AFL at the concentration of 200, 400 ug/mL significantly recovered nitric oxide production which was reduced by EtOH (100 uM) in Raw 264.7 cells.
3. AFL at the concentration of 400 ug/mL significantly recovered nitric oxide production which was reduced by nicotine (1mM) in
Raw 264.7 cells.
4. AFL at the concentration of 200, 400 ug/mL significantly recovered nitric oxide production which was reduced by acetaminophen(2mM) in Raw 264.7 cells.
5. AFL at the concentration of 200, 400 ug/mL significantly recovered nitric oxide production which was reduced by acetaldehyde (200 uM) in Raw 264.7 cells.
Conclusions : AFL could be supposed to have the immune-enhancing activity related with nitric oxide production of macrophage impaired by various toxicants. |
| first_indexed | 2024-12-22T19:56:28Z |
| format | Article |
| id | doaj.art-900bd7bb9621444e9914e20322a7142c |
| institution | Directory Open Access Journal |
| issn | 2093-6966 2234-6856 |
| language | English |
| last_indexed | 2024-12-22T19:56:28Z |
| publishDate | 2009-12-01 |
| publisher | Korean Pharmacopuncture Institute |
| record_format | Article |
| series | Journal of Pharmacopuncture |
| spelling | doaj.art-900bd7bb9621444e9914e20322a7142c2022-12-21T18:14:24ZengKorean Pharmacopuncture InstituteJournal of Pharmacopuncture2093-69662234-68562009-12-01124899610.3831/KPI.2009.12.4.089Effect of Lactobacillus pentosus-Fermented Artemisiae Argi Folium on Nitric Oxide Production of Macrophage impaired with Various ToxicantsWansu Park0Dept. of Pathology, College of Oriental Medicine, Kyungwon UniversityObjectives : The purpose of this study is to investigate the effect of Water Extract from Lactobacillus pentosus-fermented ARTEMISIAE ARGI FOLIUM (AFL) on nitric oxide production of mouse macrophage Raw 264.7 cells impaired by various toxicants such as gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde. Methods : ARTEMISIAE ARGI FOLIUM was fermented with Lactobacillus pentosus and extracted by water. Nitric oxide production of mouse macrophage Raw 264.7 cells was measured by Griess reagent assay. Examined concentrations of AFL were 10, 50, 100, 200, 400 ug/mL. Results : The results of the experiment are as below. 1. AFL at the concentration of 400 ug/mL significantly recovered nitric oxide production which was reduced by gallic acid (100 uM) in Raw 264.7 cells. 2. AFL at the concentration of 200, 400 ug/mL significantly recovered nitric oxide production which was reduced by EtOH (100 uM) in Raw 264.7 cells. 3. AFL at the concentration of 400 ug/mL significantly recovered nitric oxide production which was reduced by nicotine (1mM) in Raw 264.7 cells. 4. AFL at the concentration of 200, 400 ug/mL significantly recovered nitric oxide production which was reduced by acetaminophen(2mM) in Raw 264.7 cells. 5. AFL at the concentration of 200, 400 ug/mL significantly recovered nitric oxide production which was reduced by acetaldehyde (200 uM) in Raw 264.7 cells. Conclusions : AFL could be supposed to have the immune-enhancing activity related with nitric oxide production of macrophage impaired by various toxicants.http://dx.doi.org/10.3831/KPI.2009.12.4.089macrophageARTEMISIAE ARGI FOLIUMLactobacillus pentosusfermentationnitric oxide |
| spellingShingle | Wansu Park Effect of Lactobacillus pentosus-Fermented Artemisiae Argi Folium on Nitric Oxide Production of Macrophage impaired with Various Toxicants Journal of Pharmacopuncture macrophage ARTEMISIAE ARGI FOLIUM Lactobacillus pentosus fermentation nitric oxide |
| title | Effect of Lactobacillus pentosus-Fermented Artemisiae Argi Folium on Nitric Oxide Production of Macrophage impaired with Various Toxicants |
| title_full | Effect of Lactobacillus pentosus-Fermented Artemisiae Argi Folium on Nitric Oxide Production of Macrophage impaired with Various Toxicants |
| title_fullStr | Effect of Lactobacillus pentosus-Fermented Artemisiae Argi Folium on Nitric Oxide Production of Macrophage impaired with Various Toxicants |
| title_full_unstemmed | Effect of Lactobacillus pentosus-Fermented Artemisiae Argi Folium on Nitric Oxide Production of Macrophage impaired with Various Toxicants |
| title_short | Effect of Lactobacillus pentosus-Fermented Artemisiae Argi Folium on Nitric Oxide Production of Macrophage impaired with Various Toxicants |
| title_sort | effect of lactobacillus pentosus fermented artemisiae argi folium on nitric oxide production of macrophage impaired with various toxicants |
| topic | macrophage ARTEMISIAE ARGI FOLIUM Lactobacillus pentosus fermentation nitric oxide |
| url | http://dx.doi.org/10.3831/KPI.2009.12.4.089 |
| work_keys_str_mv | AT wansupark effectoflactobacilluspentosusfermentedartemisiaeargifoliumonnitricoxideproductionofmacrophageimpairedwithvarioustoxicants |