Genetic Diversity and Phylogenetic Relationship Analysis between Red Jungle Fowl and Domestic Chicken using AFLP Markers
Genetic variation and phylogenetic relationships among 76 birds belonging to Indian Red Jungle Fowl (RJF) and three domestic chicken breeds viz. one Indian native sport breed, Aseel (AS) and two global high yielding breeds of egg type, White Leghorn (WL) and meat type, Red Cornish (RC) were evaluate...
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2015-04-01
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| Series: | The Journal of Poultry Science |
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| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/52/2/52_0140145/_pdf/-char/en |
| _version_ | 1827938952899723264 |
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| author | Vinay Kumar Sanjeev Kumar Shukla Satyendra Kumar Singh Girraj Goyal Jose Mathew Deepak Sharma |
| author_facet | Vinay Kumar Sanjeev Kumar Shukla Satyendra Kumar Singh Girraj Goyal Jose Mathew Deepak Sharma |
| author_sort | Vinay Kumar |
| collection | DOAJ |
| description | Genetic variation and phylogenetic relationships among 76 birds belonging to Indian Red Jungle Fowl (RJF) and three domestic chicken breeds viz. one Indian native sport breed, Aseel (AS) and two global high yielding breeds of egg type, White Leghorn (WL) and meat type, Red Cornish (RC) were evaluated using Amplified Fragment Length Polymorphism (AFLP) markers. A total of 318 scorable dominant AFLP bands in the range of 50-500 bp using 20 EcoRI/TaqI primer combinations were detected across populations. The mean number of polymorphic bands across all four populations was 15.5 per primer combination. Species-specific bands were also found in all populations except WL. Nei’s gene diversity was measured between RJF and domestic chickens and it was significantly higher in RJF population (0.309) as compared to the domestic chicken populations of AS (0.129), WL (0.067) and RC (0.066). RJF showed maximum genetic distance with RC (0.221) and minimum with AS (0.177) population. The divergence between RJF and domestic chicken was observed by constructing an UPGMA dendrogram. RJF was present in one cluster and whereas the three domestic chickens were tightly clustered in another group. This clustering pattern was also confirmed by principal component analysis (PCA). The estimate of genetic identities (GI) and genetic distances (GD) using Nei (1972) also suggest comparatively more closeness of RJF with AS (an Indian native chicken breed) than those of commercial breeds WL and RC. These results support the earlier understanding that chicken was first domesticated for game purpose rather than as food. |
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| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:46:15Z |
| publishDate | 2015-04-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-900e28b8a82f4a86afae867fdf33f2c52023-05-30T04:41:33ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862015-04-015229410010.2141/jpsa.0140145jpsaGenetic Diversity and Phylogenetic Relationship Analysis between Red Jungle Fowl and Domestic Chicken using AFLP MarkersVinay Kumar0Sanjeev Kumar Shukla1Satyendra Kumar Singh2Girraj Goyal3Jose Mathew4Deepak Sharma5Avian Genetics and Breeding, Central Avian Research Institute, IndiaAvian Genetics and Breeding, Central Avian Research Institute, IndiaAvian Genetics and Breeding, Central Avian Research Institute, IndiaAvian Genetics and Breeding, Central Avian Research Institute, IndiaDepartment of Biotechnology, Bundelkhand University, IndiaAvian Genetics and Breeding, Central Avian Research Institute, IndiaGenetic variation and phylogenetic relationships among 76 birds belonging to Indian Red Jungle Fowl (RJF) and three domestic chicken breeds viz. one Indian native sport breed, Aseel (AS) and two global high yielding breeds of egg type, White Leghorn (WL) and meat type, Red Cornish (RC) were evaluated using Amplified Fragment Length Polymorphism (AFLP) markers. A total of 318 scorable dominant AFLP bands in the range of 50-500 bp using 20 EcoRI/TaqI primer combinations were detected across populations. The mean number of polymorphic bands across all four populations was 15.5 per primer combination. Species-specific bands were also found in all populations except WL. Nei’s gene diversity was measured between RJF and domestic chickens and it was significantly higher in RJF population (0.309) as compared to the domestic chicken populations of AS (0.129), WL (0.067) and RC (0.066). RJF showed maximum genetic distance with RC (0.221) and minimum with AS (0.177) population. The divergence between RJF and domestic chicken was observed by constructing an UPGMA dendrogram. RJF was present in one cluster and whereas the three domestic chickens were tightly clustered in another group. This clustering pattern was also confirmed by principal component analysis (PCA). The estimate of genetic identities (GI) and genetic distances (GD) using Nei (1972) also suggest comparatively more closeness of RJF with AS (an Indian native chicken breed) than those of commercial breeds WL and RC. These results support the earlier understanding that chicken was first domesticated for game purpose rather than as food.https://www.jstage.jst.go.jp/article/jpsa/52/2/52_0140145/_pdf/-char/enaflpgenetic diversityphylogenetic relationshipred jungle fowl |
| spellingShingle | Vinay Kumar Sanjeev Kumar Shukla Satyendra Kumar Singh Girraj Goyal Jose Mathew Deepak Sharma Genetic Diversity and Phylogenetic Relationship Analysis between Red Jungle Fowl and Domestic Chicken using AFLP Markers The Journal of Poultry Science aflp genetic diversity phylogenetic relationship red jungle fowl |
| title | Genetic Diversity and Phylogenetic Relationship Analysis between Red Jungle Fowl and Domestic Chicken using AFLP Markers |
| title_full | Genetic Diversity and Phylogenetic Relationship Analysis between Red Jungle Fowl and Domestic Chicken using AFLP Markers |
| title_fullStr | Genetic Diversity and Phylogenetic Relationship Analysis between Red Jungle Fowl and Domestic Chicken using AFLP Markers |
| title_full_unstemmed | Genetic Diversity and Phylogenetic Relationship Analysis between Red Jungle Fowl and Domestic Chicken using AFLP Markers |
| title_short | Genetic Diversity and Phylogenetic Relationship Analysis between Red Jungle Fowl and Domestic Chicken using AFLP Markers |
| title_sort | genetic diversity and phylogenetic relationship analysis between red jungle fowl and domestic chicken using aflp markers |
| topic | aflp genetic diversity phylogenetic relationship red jungle fowl |
| url | https://www.jstage.jst.go.jp/article/jpsa/52/2/52_0140145/_pdf/-char/en |
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