Integrated Metabolomic and Transcriptomic Analysis of the Flavonoid Accumulation in the Leaves of Cyclocarya paliurus at Different Altitudes
Cyclocarya paliurus is a medicinal plant containing flavonoids, triterpenoids, polyphenolics, polysaccharides, and other compounds with diverse biological functions. C. paliurus is distributed across altitudes ranging from 400 to 1,000 m. However, little is known about the effect of altitude on meta...
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Frontiers Media S.A.
2022-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.794137/full |
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| author | Zhaokui Du Weida Lin Weida Lin Binbin Yu Jinxing Zhu Junmin Li |
| author_facet | Zhaokui Du Weida Lin Weida Lin Binbin Yu Jinxing Zhu Junmin Li |
| author_sort | Zhaokui Du |
| collection | DOAJ |
| description | Cyclocarya paliurus is a medicinal plant containing flavonoids, triterpenoids, polyphenolics, polysaccharides, and other compounds with diverse biological functions. C. paliurus is distributed across altitudes ranging from 400 to 1,000 m. However, little is known about the effect of altitude on metabolite accumulation in C. paliurus. Also, the biosynthetic pathway involved in flavonoid accumulation in C. paliurus has not been fully elucidated. In this study, mature leaves of C. paliurus growing at low altitude (280 m) and high altitude (920 m) were sampled and subjected to metabolomic and transcriptomic analyses. The flavonoid content and composition were higher in the leaves of C. paliurus collected at high altitude than in those collected at low altitude. Most of the differentially accumulated metabolites (DAMs) were enriched in “flavone and flavonol biosynthesis.” The significant differentially expressed genes (DEGs) between low and high altitudes were mainly enriched in “biological process.” The most heavily enriched KEGG pathway was related to the subcategory “Oxidative phosphorylation,” indicating that complicated biological processes are involved in the response of C. paliurus to harsh environmental factors. High UV-light might be the main influencing factor among the harsh environmental factors found in high altitudes. Integrated analysis of metabolomic and transcriptomic data showed that 31 flavonoids were significantly correlated with 227 DEGs, resulting in 412 related pairs (283 positive and 129 negative) between the DEGs and flavonoids. The possible mechanisms underlying the differentially accumulation of flavonoids at different altitude might be due to variations in transport and relocation of flavonoids in C. paliurus leaves, but not different flavonoid biosynthesis pathways. The up-regulation of genes related to energy and protein synthesis might contribute to flavonoid accumulation at high altitudes. This study broadens our understanding of the effect of altitude on metabolite accumulation and biosynthesis in C. paliurus. |
| first_indexed | 2024-12-24T01:48:56Z |
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| language | English |
| last_indexed | 2024-12-24T01:48:56Z |
| publishDate | 2022-02-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-900e981402cc44e3aa603f18fa7264502022-12-21T17:21:47ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-02-011210.3389/fpls.2021.794137794137Integrated Metabolomic and Transcriptomic Analysis of the Flavonoid Accumulation in the Leaves of Cyclocarya paliurus at Different AltitudesZhaokui Du0Weida Lin1Weida Lin2Binbin Yu3Jinxing Zhu4Junmin Li5Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation, Taizhou University, Taizhou, ChinaZhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation, Taizhou University, Taizhou, ChinaTaizhou Vocational College of Science and Technology, Taizhou, ChinaZhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation, Taizhou University, Taizhou, ChinaSuichang County Bureau of Agriculture and Rural Affairs, Lishui, ChinaZhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation, Taizhou University, Taizhou, ChinaCyclocarya paliurus is a medicinal plant containing flavonoids, triterpenoids, polyphenolics, polysaccharides, and other compounds with diverse biological functions. C. paliurus is distributed across altitudes ranging from 400 to 1,000 m. However, little is known about the effect of altitude on metabolite accumulation in C. paliurus. Also, the biosynthetic pathway involved in flavonoid accumulation in C. paliurus has not been fully elucidated. In this study, mature leaves of C. paliurus growing at low altitude (280 m) and high altitude (920 m) were sampled and subjected to metabolomic and transcriptomic analyses. The flavonoid content and composition were higher in the leaves of C. paliurus collected at high altitude than in those collected at low altitude. Most of the differentially accumulated metabolites (DAMs) were enriched in “flavone and flavonol biosynthesis.” The significant differentially expressed genes (DEGs) between low and high altitudes were mainly enriched in “biological process.” The most heavily enriched KEGG pathway was related to the subcategory “Oxidative phosphorylation,” indicating that complicated biological processes are involved in the response of C. paliurus to harsh environmental factors. High UV-light might be the main influencing factor among the harsh environmental factors found in high altitudes. Integrated analysis of metabolomic and transcriptomic data showed that 31 flavonoids were significantly correlated with 227 DEGs, resulting in 412 related pairs (283 positive and 129 negative) between the DEGs and flavonoids. The possible mechanisms underlying the differentially accumulation of flavonoids at different altitude might be due to variations in transport and relocation of flavonoids in C. paliurus leaves, but not different flavonoid biosynthesis pathways. The up-regulation of genes related to energy and protein synthesis might contribute to flavonoid accumulation at high altitudes. This study broadens our understanding of the effect of altitude on metabolite accumulation and biosynthesis in C. paliurus.https://www.frontiersin.org/articles/10.3389/fpls.2021.794137/fullflaovnoidmetabolometranscriptomealtitudeCyclocarya paliurus |
| spellingShingle | Zhaokui Du Weida Lin Weida Lin Binbin Yu Jinxing Zhu Junmin Li Integrated Metabolomic and Transcriptomic Analysis of the Flavonoid Accumulation in the Leaves of Cyclocarya paliurus at Different Altitudes Frontiers in Plant Science flaovnoid metabolome transcriptome altitude Cyclocarya paliurus |
| title | Integrated Metabolomic and Transcriptomic Analysis of the Flavonoid Accumulation in the Leaves of Cyclocarya paliurus at Different Altitudes |
| title_full | Integrated Metabolomic and Transcriptomic Analysis of the Flavonoid Accumulation in the Leaves of Cyclocarya paliurus at Different Altitudes |
| title_fullStr | Integrated Metabolomic and Transcriptomic Analysis of the Flavonoid Accumulation in the Leaves of Cyclocarya paliurus at Different Altitudes |
| title_full_unstemmed | Integrated Metabolomic and Transcriptomic Analysis of the Flavonoid Accumulation in the Leaves of Cyclocarya paliurus at Different Altitudes |
| title_short | Integrated Metabolomic and Transcriptomic Analysis of the Flavonoid Accumulation in the Leaves of Cyclocarya paliurus at Different Altitudes |
| title_sort | integrated metabolomic and transcriptomic analysis of the flavonoid accumulation in the leaves of cyclocarya paliurus at different altitudes |
| topic | flaovnoid metabolome transcriptome altitude Cyclocarya paliurus |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.794137/full |
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