Virulence Determinants in Staphylococcus aureus Clones Causing Osteomyelitis in Italy
Staphylococcus aureus is the most common pathogen causing osteomyelitis (OM). The aim of this study was to explore the clonal complex (CC) distribution and the pattern of virulence determinants of S. aureus isolates from OM in Italy. Whole-genome sequencing was performed on 83 S. aureus isolates fro...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.846167/full |
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| author | Fernanda Pimentel de Araujo Fernanda Pimentel de Araujo Mattia Pirolo Monica Monaco Maria Del Grosso Simone Ambretti Donatella Lombardo Tiziana Cassetti Raffaele Gargiulo Eleonora Riccobono Paolo Visca Paolo Visca Annalisa Pantosti |
| author_facet | Fernanda Pimentel de Araujo Fernanda Pimentel de Araujo Mattia Pirolo Monica Monaco Maria Del Grosso Simone Ambretti Donatella Lombardo Tiziana Cassetti Raffaele Gargiulo Eleonora Riccobono Paolo Visca Paolo Visca Annalisa Pantosti |
| author_sort | Fernanda Pimentel de Araujo |
| collection | DOAJ |
| description | Staphylococcus aureus is the most common pathogen causing osteomyelitis (OM). The aim of this study was to explore the clonal complex (CC) distribution and the pattern of virulence determinants of S. aureus isolates from OM in Italy. Whole-genome sequencing was performed on 83 S. aureus isolates from OM cases in six hospitals. Antibiotic susceptibility tests showed that 30.1% of the isolates were methicillin-resistant S. aureus (MRSA). The most frequent CCs detected were CC22, CC5, CC8, CC30, and CC15, which represent the most common lineages circulating in Italian hospitals. MRSA were limited in the number of lineages (CC22, CC5, CC8, and CC1). Phylogenetic analysis followed the sequence type-CC groupings and revealed a non-uniform distribution of the isolates from the different hospitals. No significant difference in the mean number of virulence genes carried by MRSA or MSSA isolates was observed. Some virulence genes, namely cna, fib, fnbA, coa, lukD, lukE, sak, and tst, were correlated with the CC. However, different categories of virulence factors, such as adhesins, exoenzymes, and toxins, were frequently detected and unevenly distributed among all lineages. Indeed, each lineage carried a variable combination of virulence genes, likely reflecting functional redundancy, and arguing for the importance of those traits for the pathogenicity in OM. In conclusion, no specific genetic trait in the most frequent lineages could explain their high prevalence among OM isolates. Our findings highlight that CCs detected in OM isolates follow the epidemiology of S. aureus infections in the country. It is conceivable that any of the most common S. aureus CC can cause a variety of infections, including OM. |
| first_indexed | 2024-12-12T02:27:05Z |
| format | Article |
| id | doaj.art-900fa4a8f1d04b1986450ef0c5a7a74c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T02:27:05Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-900fa4a8f1d04b1986450ef0c5a7a74c2022-12-22T00:41:31ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-03-011310.3389/fmicb.2022.846167846167Virulence Determinants in Staphylococcus aureus Clones Causing Osteomyelitis in ItalyFernanda Pimentel de Araujo0Fernanda Pimentel de Araujo1Mattia Pirolo2Monica Monaco3Maria Del Grosso4Simone Ambretti5Donatella Lombardo6Tiziana Cassetti7Raffaele Gargiulo8Eleonora Riccobono9Paolo Visca10Paolo Visca11Annalisa Pantosti12Department of Infectious Diseases, Istituto Superiore di Sanità, Rome, ItalyDepartment of Science, Roma Tre University, Rome, ItalyDepartment of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, DenmarkDepartment of Infectious Diseases, Istituto Superiore di Sanità, Rome, ItalyDepartment of Infectious Diseases, Istituto Superiore di Sanità, Rome, ItalyUnit of Microbiology, Policlinico S. Orsola, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, ItalyUnit of Microbiology, Policlinico S. Orsola, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, ItalyUnit of Clinical Microbiology, S. Agostino-Estense Hospital Baggiovara, AUSL Modena, Modena, ItalyUnit of Clinical Microbiology, S. Agostino-Estense Hospital Baggiovara, AUSL Modena, Modena, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, Florence, ItalyDepartment of Science, Roma Tre University, Rome, ItalySanta Lucia Foundation (IRCCS), Rome, ItalyDepartment of Infectious Diseases, Istituto Superiore di Sanità, Rome, ItalyStaphylococcus aureus is the most common pathogen causing osteomyelitis (OM). The aim of this study was to explore the clonal complex (CC) distribution and the pattern of virulence determinants of S. aureus isolates from OM in Italy. Whole-genome sequencing was performed on 83 S. aureus isolates from OM cases in six hospitals. Antibiotic susceptibility tests showed that 30.1% of the isolates were methicillin-resistant S. aureus (MRSA). The most frequent CCs detected were CC22, CC5, CC8, CC30, and CC15, which represent the most common lineages circulating in Italian hospitals. MRSA were limited in the number of lineages (CC22, CC5, CC8, and CC1). Phylogenetic analysis followed the sequence type-CC groupings and revealed a non-uniform distribution of the isolates from the different hospitals. No significant difference in the mean number of virulence genes carried by MRSA or MSSA isolates was observed. Some virulence genes, namely cna, fib, fnbA, coa, lukD, lukE, sak, and tst, were correlated with the CC. However, different categories of virulence factors, such as adhesins, exoenzymes, and toxins, were frequently detected and unevenly distributed among all lineages. Indeed, each lineage carried a variable combination of virulence genes, likely reflecting functional redundancy, and arguing for the importance of those traits for the pathogenicity in OM. In conclusion, no specific genetic trait in the most frequent lineages could explain their high prevalence among OM isolates. Our findings highlight that CCs detected in OM isolates follow the epidemiology of S. aureus infections in the country. It is conceivable that any of the most common S. aureus CC can cause a variety of infections, including OM.https://www.frontiersin.org/articles/10.3389/fmicb.2022.846167/fullStaphylococcus aureusvirulence genesosteomyelitisclonesantibiotic resistancewhole genome sequencing |
| spellingShingle | Fernanda Pimentel de Araujo Fernanda Pimentel de Araujo Mattia Pirolo Monica Monaco Maria Del Grosso Simone Ambretti Donatella Lombardo Tiziana Cassetti Raffaele Gargiulo Eleonora Riccobono Paolo Visca Paolo Visca Annalisa Pantosti Virulence Determinants in Staphylococcus aureus Clones Causing Osteomyelitis in Italy Frontiers in Microbiology Staphylococcus aureus virulence genes osteomyelitis clones antibiotic resistance whole genome sequencing |
| title | Virulence Determinants in Staphylococcus aureus Clones Causing Osteomyelitis in Italy |
| title_full | Virulence Determinants in Staphylococcus aureus Clones Causing Osteomyelitis in Italy |
| title_fullStr | Virulence Determinants in Staphylococcus aureus Clones Causing Osteomyelitis in Italy |
| title_full_unstemmed | Virulence Determinants in Staphylococcus aureus Clones Causing Osteomyelitis in Italy |
| title_short | Virulence Determinants in Staphylococcus aureus Clones Causing Osteomyelitis in Italy |
| title_sort | virulence determinants in staphylococcus aureus clones causing osteomyelitis in italy |
| topic | Staphylococcus aureus virulence genes osteomyelitis clones antibiotic resistance whole genome sequencing |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.846167/full |
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