The GhMAP3K62-GhMKK16-GhMPK32 kinase cascade regulates drought tolerance by activating GhEDT1-mediated ABA accumulation in cotton
Introduction: Drought is the principal abiotic stress that severely impacts cotton (Gossypium hirsutum) growth and productivity. Upon sensing drought, plants activate stress-related signal transduction pathways, including ABA signal and mitogen-activated protein kinase (MAPK) cascade. However, as th...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-09-01
|
| Series: | Journal of Advanced Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2090123222002491 |
| _version_ | 1797729984139231232 |
|---|---|
| author | Lin Chen Bing Zhang Linjie Xia Dandan Yue Bei Han Weinan Sun Fengjiao Wang Keith Lindsey Xianlong Zhang Xiyan Yang |
| author_facet | Lin Chen Bing Zhang Linjie Xia Dandan Yue Bei Han Weinan Sun Fengjiao Wang Keith Lindsey Xianlong Zhang Xiyan Yang |
| author_sort | Lin Chen |
| collection | DOAJ |
| description | Introduction: Drought is the principal abiotic stress that severely impacts cotton (Gossypium hirsutum) growth and productivity. Upon sensing drought, plants activate stress-related signal transduction pathways, including ABA signal and mitogen-activated protein kinase (MAPK) cascade. However, as the key components with the fewest members in the MAPK cascade, the function and regulation of GhMKKs need to be elucidated. In addition, the relationship between MAPK module and the ABA core signaling pathway remains incompletely understood. Objective: Here we aim to elucidate the molecular mechanism of cotton response to drought, with a focus on mitogen-activated protein kinase (MAPK) cascades activating ABA signaling. Methods: Biochemical, molecular and genetic analysis were used to study the GhMAP3K62-GhMKK16-GhMPK32-GhEDT1 pathway genes. Results: A nucleus- and membrane-localized MAPK cascade pathway GhMAP3K62-GhMKK16-GhMPK32, which targets and phosphorylates the nuclear-localized transcription factor GhEDT1, to activate downstream GhNCED3 to mediate ABA-induced stomatal closure and drought response was characterized in cotton. Overexpression of GhMKK16 promotes ABA accumulation, and enhances drought tolerance via regulating stomatal closure under drought stress. Conversely, RNAi-mediated knockdown of GhMKK16 expression inhibits ABA accumulation, and reduces drought tolerance. Virus-induced gene silencing (VIGS)-mediated knockdown of either GhMAP3K62, GhMPK32 or GhEDT1 expression represses ABA accumulation and reduces drought tolerance through inhibiting stomatal closure. Expression knockdown of GhMPK32 or GhEDT1 in GhMKK16-overexpressing cotton reinstates ABA content and stomatal opening-dependent drought sensitivity to wild type levels. GhEDT1 could bind to the HD boxes in the promoter of GhNCED3 to activate its expression, resulting in ABA accumulation. We propose that the MAPK cascade GhMAP3K62-GhMKK16-GhMPK32 pathway functions on drought response through ABA-dependent stomatal movement in cotton. |
| first_indexed | 2024-03-12T11:37:30Z |
| format | Article |
| id | doaj.art-9014b018519e4d6c8db649022964f37e |
| institution | Directory Open Access Journal |
| issn | 2090-1232 |
| language | English |
| last_indexed | 2024-03-12T11:37:30Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Advanced Research |
| spelling | doaj.art-9014b018519e4d6c8db649022964f37e2023-09-01T05:01:02ZengElsevierJournal of Advanced Research2090-12322023-09-01511325The GhMAP3K62-GhMKK16-GhMPK32 kinase cascade regulates drought tolerance by activating GhEDT1-mediated ABA accumulation in cottonLin Chen0Bing Zhang1Linjie Xia2Dandan Yue3Bei Han4Weinan Sun5Fengjiao Wang6Keith Lindsey7Xianlong Zhang8Xiyan Yang9National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei 430070, P. R. ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei 430070, P. R. China; Hubei Hongshan Laboratory, Wuhan, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei 430070, P. R. China; Hubei Hongshan Laboratory, Wuhan, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei 430070, P. R. China; Hubei Hongshan Laboratory, Wuhan, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei 430070, P. R. China; Hubei Hongshan Laboratory, Wuhan, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei 430070, P. R. China; Hubei Hongshan Laboratory, Wuhan, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei 430070, P. R. ChinaDepartment of Biosciences, Durham University, South Road, Durham DH1 3LE, UKNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei 430070, P. R. China; Hubei Hongshan Laboratory, Wuhan, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei 430070, P. R. China; Hubei Hongshan Laboratory, Wuhan, China; Corresponding author.Introduction: Drought is the principal abiotic stress that severely impacts cotton (Gossypium hirsutum) growth and productivity. Upon sensing drought, plants activate stress-related signal transduction pathways, including ABA signal and mitogen-activated protein kinase (MAPK) cascade. However, as the key components with the fewest members in the MAPK cascade, the function and regulation of GhMKKs need to be elucidated. In addition, the relationship between MAPK module and the ABA core signaling pathway remains incompletely understood. Objective: Here we aim to elucidate the molecular mechanism of cotton response to drought, with a focus on mitogen-activated protein kinase (MAPK) cascades activating ABA signaling. Methods: Biochemical, molecular and genetic analysis were used to study the GhMAP3K62-GhMKK16-GhMPK32-GhEDT1 pathway genes. Results: A nucleus- and membrane-localized MAPK cascade pathway GhMAP3K62-GhMKK16-GhMPK32, which targets and phosphorylates the nuclear-localized transcription factor GhEDT1, to activate downstream GhNCED3 to mediate ABA-induced stomatal closure and drought response was characterized in cotton. Overexpression of GhMKK16 promotes ABA accumulation, and enhances drought tolerance via regulating stomatal closure under drought stress. Conversely, RNAi-mediated knockdown of GhMKK16 expression inhibits ABA accumulation, and reduces drought tolerance. Virus-induced gene silencing (VIGS)-mediated knockdown of either GhMAP3K62, GhMPK32 or GhEDT1 expression represses ABA accumulation and reduces drought tolerance through inhibiting stomatal closure. Expression knockdown of GhMPK32 or GhEDT1 in GhMKK16-overexpressing cotton reinstates ABA content and stomatal opening-dependent drought sensitivity to wild type levels. GhEDT1 could bind to the HD boxes in the promoter of GhNCED3 to activate its expression, resulting in ABA accumulation. We propose that the MAPK cascade GhMAP3K62-GhMKK16-GhMPK32 pathway functions on drought response through ABA-dependent stomatal movement in cotton.http://www.sciencedirect.com/science/article/pii/S2090123222002491ABACottonDroughtGhEDT1MAPK cascade pathwayStomatal movement |
| spellingShingle | Lin Chen Bing Zhang Linjie Xia Dandan Yue Bei Han Weinan Sun Fengjiao Wang Keith Lindsey Xianlong Zhang Xiyan Yang The GhMAP3K62-GhMKK16-GhMPK32 kinase cascade regulates drought tolerance by activating GhEDT1-mediated ABA accumulation in cotton Journal of Advanced Research ABA Cotton Drought GhEDT1 MAPK cascade pathway Stomatal movement |
| title | The GhMAP3K62-GhMKK16-GhMPK32 kinase cascade regulates drought tolerance by activating GhEDT1-mediated ABA accumulation in cotton |
| title_full | The GhMAP3K62-GhMKK16-GhMPK32 kinase cascade regulates drought tolerance by activating GhEDT1-mediated ABA accumulation in cotton |
| title_fullStr | The GhMAP3K62-GhMKK16-GhMPK32 kinase cascade regulates drought tolerance by activating GhEDT1-mediated ABA accumulation in cotton |
| title_full_unstemmed | The GhMAP3K62-GhMKK16-GhMPK32 kinase cascade regulates drought tolerance by activating GhEDT1-mediated ABA accumulation in cotton |
| title_short | The GhMAP3K62-GhMKK16-GhMPK32 kinase cascade regulates drought tolerance by activating GhEDT1-mediated ABA accumulation in cotton |
| title_sort | ghmap3k62 ghmkk16 ghmpk32 kinase cascade regulates drought tolerance by activating ghedt1 mediated aba accumulation in cotton |
| topic | ABA Cotton Drought GhEDT1 MAPK cascade pathway Stomatal movement |
| url | http://www.sciencedirect.com/science/article/pii/S2090123222002491 |
| work_keys_str_mv | AT linchen theghmap3k62ghmkk16ghmpk32kinasecascaderegulatesdroughttolerancebyactivatingghedt1mediatedabaaccumulationincotton AT bingzhang theghmap3k62ghmkk16ghmpk32kinasecascaderegulatesdroughttolerancebyactivatingghedt1mediatedabaaccumulationincotton AT linjiexia theghmap3k62ghmkk16ghmpk32kinasecascaderegulatesdroughttolerancebyactivatingghedt1mediatedabaaccumulationincotton AT dandanyue theghmap3k62ghmkk16ghmpk32kinasecascaderegulatesdroughttolerancebyactivatingghedt1mediatedabaaccumulationincotton AT beihan theghmap3k62ghmkk16ghmpk32kinasecascaderegulatesdroughttolerancebyactivatingghedt1mediatedabaaccumulationincotton AT weinansun theghmap3k62ghmkk16ghmpk32kinasecascaderegulatesdroughttolerancebyactivatingghedt1mediatedabaaccumulationincotton AT fengjiaowang theghmap3k62ghmkk16ghmpk32kinasecascaderegulatesdroughttolerancebyactivatingghedt1mediatedabaaccumulationincotton AT keithlindsey theghmap3k62ghmkk16ghmpk32kinasecascaderegulatesdroughttolerancebyactivatingghedt1mediatedabaaccumulationincotton AT xianlongzhang theghmap3k62ghmkk16ghmpk32kinasecascaderegulatesdroughttolerancebyactivatingghedt1mediatedabaaccumulationincotton AT xiyanyang theghmap3k62ghmkk16ghmpk32kinasecascaderegulatesdroughttolerancebyactivatingghedt1mediatedabaaccumulationincotton AT linchen ghmap3k62ghmkk16ghmpk32kinasecascaderegulatesdroughttolerancebyactivatingghedt1mediatedabaaccumulationincotton AT bingzhang ghmap3k62ghmkk16ghmpk32kinasecascaderegulatesdroughttolerancebyactivatingghedt1mediatedabaaccumulationincotton AT linjiexia ghmap3k62ghmkk16ghmpk32kinasecascaderegulatesdroughttolerancebyactivatingghedt1mediatedabaaccumulationincotton AT dandanyue ghmap3k62ghmkk16ghmpk32kinasecascaderegulatesdroughttolerancebyactivatingghedt1mediatedabaaccumulationincotton AT beihan ghmap3k62ghmkk16ghmpk32kinasecascaderegulatesdroughttolerancebyactivatingghedt1mediatedabaaccumulationincotton AT weinansun ghmap3k62ghmkk16ghmpk32kinasecascaderegulatesdroughttolerancebyactivatingghedt1mediatedabaaccumulationincotton AT fengjiaowang ghmap3k62ghmkk16ghmpk32kinasecascaderegulatesdroughttolerancebyactivatingghedt1mediatedabaaccumulationincotton AT keithlindsey ghmap3k62ghmkk16ghmpk32kinasecascaderegulatesdroughttolerancebyactivatingghedt1mediatedabaaccumulationincotton AT xianlongzhang ghmap3k62ghmkk16ghmpk32kinasecascaderegulatesdroughttolerancebyactivatingghedt1mediatedabaaccumulationincotton AT xiyanyang ghmap3k62ghmkk16ghmpk32kinasecascaderegulatesdroughttolerancebyactivatingghedt1mediatedabaaccumulationincotton |