Expression of Notch signaling pathway in temporomandibular osteoarthritis
Objective To investigate the expression of Notch signaling molecules in temporomandibular joint ar⁃ thritis (TMJOA), and to explore the role and mechanism of Notch signaling pathway in the development and progression of TMJOA. Methods 72 Kunming mice were randomly divided into experimental group,...
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases
2017-03-01
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| Series: | 口腔疾病防治 |
| Subjects: | |
| Online Access: | http://www.kqjbfz.com/EN/10.12016/j.issn.2096-1456.2017.03.002 |
| Summary: | Objective To investigate the expression of Notch signaling molecules in temporomandibular joint ar⁃
thritis (TMJOA), and to explore the role and mechanism of Notch signaling pathway in the development and progression
of TMJOA. Methods 72 Kunming mice were randomly divided into experimental group, sham⁃surgery group and nor⁃
mal group. In experimental group, discs on the right TMJ were subjected to total discectomy, sham⁃operation group un⁃
derwent the same procedure without disc removal, while normal group serve as blank control. All the left temporoman⁃
dibular joint discs were not treated. 8 mice in each group were sacrificed respectively at 1 week, 2 week, 4 week after
surgery. Histological examinations were performed to assess success of TMJOA model, according to the pathological
standard of osteoarthritis diagnosis. Immunochemistry techniques were performed in the successful TMJOA cartilages to
evaluate the expression levels of Notch1 (NICD1), Jagged1, Hes1 and Hes5. The scores were evaluated by semi⁃quantita⁃
tive method. Results Notch1 (NICD1), Jagged1 and Hes5 were activated in the experimental group with the expression
levels increased dramatically over time. While Hes1 expression was suppressed at the beginning of osteoarthritis but
was up⁃regulated afterwards. Conclusion Notch pathway⁃related molecular expression changed greatly in TMJOA mod⁃
el, indicating that the pathway in the occurrence and development of TMJOA plays an important role. |
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| ISSN: | 2096-1456 2096-1456 |