Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture.
The predilection of Plasmodium vivax (P. vivax) for reticulocytes is a major obstacle for its establishment in a long-term culture system, as this requires a continuous supply of large quantities of reticulocytes, representing only 1-2% of circulating red blood cells. We here compared the production...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4231068?pdf=render |
| _version_ | 1819262789487689728 |
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| author | Florian Noulin Javed Karim Manesia Anna Rosanas-Urgell Annette Erhart Céline Borlon Jan Van Den Abbeele Umberto d'Alessandro Catherine M Verfaillie |
| author_facet | Florian Noulin Javed Karim Manesia Anna Rosanas-Urgell Annette Erhart Céline Borlon Jan Van Den Abbeele Umberto d'Alessandro Catherine M Verfaillie |
| author_sort | Florian Noulin |
| collection | DOAJ |
| description | The predilection of Plasmodium vivax (P. vivax) for reticulocytes is a major obstacle for its establishment in a long-term culture system, as this requires a continuous supply of large quantities of reticulocytes, representing only 1-2% of circulating red blood cells. We here compared the production of reticulocytes using an established in vitro culture system from three different sources of hematopoietic stem/progenitor cells (HSPC), i.e. umbilical cord blood (UCB), bone marrow (BM) and adult peripheral blood (PB). Compared to CD34+-enriched populations of PB and BM, CD34+-enriched populations of UCB produced the highest amount of reticulocytes that could be invaded by P. vivax. In addition, when CD34+-enriched cells were first expanded, a further extensive increase in reticulocytes was seen for UCB, to a lesser degree BM but not PB. As invasion by P. vivax was significantly better in reticulocytes generated in vitro, we also suggest that P. vivax may have a preference for invading immature reticulocytes, which should be confirmed in future studies. |
| first_indexed | 2024-12-23T20:03:17Z |
| format | Article |
| id | doaj.art-9019142f87164e0c970120c6a04477e3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T20:03:17Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9019142f87164e0c970120c6a04477e32022-12-21T17:33:01ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01911e11249610.1371/journal.pone.0112496Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture.Florian NoulinJaved Karim ManesiaAnna Rosanas-UrgellAnnette ErhartCéline BorlonJan Van Den AbbeeleUmberto d'AlessandroCatherine M VerfaillieThe predilection of Plasmodium vivax (P. vivax) for reticulocytes is a major obstacle for its establishment in a long-term culture system, as this requires a continuous supply of large quantities of reticulocytes, representing only 1-2% of circulating red blood cells. We here compared the production of reticulocytes using an established in vitro culture system from three different sources of hematopoietic stem/progenitor cells (HSPC), i.e. umbilical cord blood (UCB), bone marrow (BM) and adult peripheral blood (PB). Compared to CD34+-enriched populations of PB and BM, CD34+-enriched populations of UCB produced the highest amount of reticulocytes that could be invaded by P. vivax. In addition, when CD34+-enriched cells were first expanded, a further extensive increase in reticulocytes was seen for UCB, to a lesser degree BM but not PB. As invasion by P. vivax was significantly better in reticulocytes generated in vitro, we also suggest that P. vivax may have a preference for invading immature reticulocytes, which should be confirmed in future studies.http://europepmc.org/articles/PMC4231068?pdf=render |
| spellingShingle | Florian Noulin Javed Karim Manesia Anna Rosanas-Urgell Annette Erhart Céline Borlon Jan Van Den Abbeele Umberto d'Alessandro Catherine M Verfaillie Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture. PLoS ONE |
| title | Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture. |
| title_full | Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture. |
| title_fullStr | Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture. |
| title_full_unstemmed | Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture. |
| title_short | Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture. |
| title_sort | hematopoietic stem progenitor cell sources to generate reticulocytes for plasmodium vivax culture |
| url | http://europepmc.org/articles/PMC4231068?pdf=render |
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