Cell viability and bioactive coating on additive manufactured polycaprolactone substrate for osteointegration applications
Polycaprolactone (PCL) finds its application in the medical field in the areas of suture materials, scaffolds for tissue engineering and medical devices fabrication. To improve the anchoring or osteointegration of PCL, bioactive material will be reinforced or deposited as a layer over the PCL substr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Chemical Physics Impact |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667022423002487 |
| _version_ | 1827221665378992128 |
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| author | K Hariharan M. Selvakumar T. Ramkumar P. Chandramohan |
| author_facet | K Hariharan M. Selvakumar T. Ramkumar P. Chandramohan |
| author_sort | K Hariharan |
| collection | DOAJ |
| description | Polycaprolactone (PCL) finds its application in the medical field in the areas of suture materials, scaffolds for tissue engineering and medical devices fabrication. To improve the anchoring or osteointegration of PCL, bioactive material will be reinforced or deposited as a layer over the PCL substrate. This experimental work aims to deposit Hydroxyapatite (HA),a bioactive material over PCL substrate material using Electron Beam Deposition (EBD). The results of the characterization studies reveal that the HA was successfully deposited over PCL substrate with suitable crystalline and surface roughness. It was also evident that Ca/P ratio of deposited HA was reasonably closer to that of standard stoichiometric HA. The HA layer deposited by Pulsed Laser Deposition (PLD) process could be an interesting option for enhancing the biocompatibility of additive manufactured polyamide. The results revealed that the water contact of HA coated PCL, the substrate has an angle of 113° with a hydrophobic surface and surface energy of 14.32 mJ/m2. However, the cell growth on coated sample was increased by 22 % and 45 % for 7th and 15th day respectively. This increment in coated sample was due to release of Ca2+ ions Fluorescent microscopic image of cells seeded on coated sample clearly revealed that the viable cells (green in colour) growth was improved in coated samples. |
| first_indexed | 2024-03-09T02:12:50Z |
| format | Article |
| id | doaj.art-901e107328694f938bd7fab522482fc2 |
| institution | Directory Open Access Journal |
| issn | 2667-0224 |
| language | English |
| last_indexed | 2025-03-21T16:26:12Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Chemical Physics Impact |
| spelling | doaj.art-901e107328694f938bd7fab522482fc22024-06-17T05:59:29ZengElsevierChemical Physics Impact2667-02242024-06-018100409Cell viability and bioactive coating on additive manufactured polycaprolactone substrate for osteointegration applicationsK Hariharan0M. Selvakumar1T. Ramkumar2P. Chandramohan3Department of Mechanical Engineering, Dr. Mahalingam College of Engineering and Technology, Pollachi 642003, IndiaDepartment of Automobile Engineering, Dr. Mahalingam College of Engineering and Technology, Pollachi 642003, IndiaDepartment of Mechanical Engineering, Dr. Mahalingam College of Engineering and Technology, Pollachi 642003, IndiaDepartment of Mechanical Engineering, Sri Ramakrishna Engineering College, Coimbatore 641022, India; Department of Metallurgy, University of Johannesburg, Johannesburg 2028, South Africa; Corresponding author at: Department of Mechanical Engineering, Sri Ramakrishna Engineering College, Coimbatore 641022, India.Polycaprolactone (PCL) finds its application in the medical field in the areas of suture materials, scaffolds for tissue engineering and medical devices fabrication. To improve the anchoring or osteointegration of PCL, bioactive material will be reinforced or deposited as a layer over the PCL substrate. This experimental work aims to deposit Hydroxyapatite (HA),a bioactive material over PCL substrate material using Electron Beam Deposition (EBD). The results of the characterization studies reveal that the HA was successfully deposited over PCL substrate with suitable crystalline and surface roughness. It was also evident that Ca/P ratio of deposited HA was reasonably closer to that of standard stoichiometric HA. The HA layer deposited by Pulsed Laser Deposition (PLD) process could be an interesting option for enhancing the biocompatibility of additive manufactured polyamide. The results revealed that the water contact of HA coated PCL, the substrate has an angle of 113° with a hydrophobic surface and surface energy of 14.32 mJ/m2. However, the cell growth on coated sample was increased by 22 % and 45 % for 7th and 15th day respectively. This increment in coated sample was due to release of Ca2+ ions Fluorescent microscopic image of cells seeded on coated sample clearly revealed that the viable cells (green in colour) growth was improved in coated samples.http://www.sciencedirect.com/science/article/pii/S2667022423002487BiomaterialsAdditive manufacturingScaffoldsPLDPCL |
| spellingShingle | K Hariharan M. Selvakumar T. Ramkumar P. Chandramohan Cell viability and bioactive coating on additive manufactured polycaprolactone substrate for osteointegration applications Chemical Physics Impact Biomaterials Additive manufacturing Scaffolds PLD PCL |
| title | Cell viability and bioactive coating on additive manufactured polycaprolactone substrate for osteointegration applications |
| title_full | Cell viability and bioactive coating on additive manufactured polycaprolactone substrate for osteointegration applications |
| title_fullStr | Cell viability and bioactive coating on additive manufactured polycaprolactone substrate for osteointegration applications |
| title_full_unstemmed | Cell viability and bioactive coating on additive manufactured polycaprolactone substrate for osteointegration applications |
| title_short | Cell viability and bioactive coating on additive manufactured polycaprolactone substrate for osteointegration applications |
| title_sort | cell viability and bioactive coating on additive manufactured polycaprolactone substrate for osteointegration applications |
| topic | Biomaterials Additive manufacturing Scaffolds PLD PCL |
| url | http://www.sciencedirect.com/science/article/pii/S2667022423002487 |
| work_keys_str_mv | AT khariharan cellviabilityandbioactivecoatingonadditivemanufacturedpolycaprolactonesubstrateforosteointegrationapplications AT mselvakumar cellviabilityandbioactivecoatingonadditivemanufacturedpolycaprolactonesubstrateforosteointegrationapplications AT tramkumar cellviabilityandbioactivecoatingonadditivemanufacturedpolycaprolactonesubstrateforosteointegrationapplications AT pchandramohan cellviabilityandbioactivecoatingonadditivemanufacturedpolycaprolactonesubstrateforosteointegrationapplications |