Amino Acids Composition and Antioxidant Activity of Selected Mushrooms from Bosnia and Herzegovina
Many studies highlight the health benefits of mushrooms, which are consequently becoming more and more interesting for researchers. The content of amino acids (AA), total phenolic content (TPC), and antioxidative activity (AAc) were determined in wild as well as cultivated mushrooms. The AA included...
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| Format: | Article |
| Language: | English |
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Croatian Society of Chemical Engineers
2019-03-01
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| Series: | Kemija u Industriji |
| Subjects: | |
| Online Access: | http://silverstripe.fkit.hr/kui/assets/Uploads/I-97-103.pdf |
| _version_ | 1828860939426856960 |
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| author | Mirsada Salihović Aida Šapčanin Ekrem Pehlić Alija Uzunović Selma Špirtović-Halilović Melita Huremović |
| author_facet | Mirsada Salihović Aida Šapčanin Ekrem Pehlić Alija Uzunović Selma Špirtović-Halilović Melita Huremović |
| author_sort | Mirsada Salihović |
| collection | DOAJ |
| description | Many studies highlight the health benefits of mushrooms, which are consequently becoming more and more interesting for researchers. The content of amino acids (AA), total phenolic content (TPC), and antioxidative activity (AAc) were determined in wild as well as cultivated mushrooms. The AA included: L-tryptophan (Trp), L-arginine (Arg), L-cysteine (Cys), L-methionine (Met), L-alanine (Ala), L-phenylalanine (Phe), L-lysine (Lys), L-valine (Val), L-glycine (Gly), and L-leucine (Leu). The wild mushrooms: Lactarius piperatus, Amanita caesarea, Lactarius deliciosus, Lycoperdon pyriforme, Macrolepiota procera, and cultivated mushrooms: Agaricus bisporus, Boletus aestivalis, Cantharellus cibarius, Pleurotus ostreatus, and Agaricus bisporus var. avellaneus were investigated in this study. The AA was determined by HPTLC method and quantified with a Camag TLC scanner and WINCAT software by scanning the plates at 540 nm. The TPC was spectrophotometrically estimated as gallic acid equivalents/g of fresh weight according to Folin-Ciocalteu’s method. The radical scavenging activity (RSA) of mushroom extracts was determined by DPPH assay. The highest content of Ala, Gly, Phe, Lys, Val, and Leu was found in Pleurotus ostreatus. The total phenolic content (mg GAE/g) in investigated mushrooms ranged from 1.90 to 35.56, and the % RSA ranged from 43.88 to 90.17. This study promotes the consumption of food rich in bioactive compounds, mushrooms being among such food. Further research on mushrooms from Bosnia and Herzegovina and their benefits in the overall maintenance of human health and protection from age-related diseases is necessary. |
| first_indexed | 2024-12-13T02:50:18Z |
| format | Article |
| id | doaj.art-901e3b4289164591925c81936f12e2d9 |
| institution | Directory Open Access Journal |
| issn | 0022-9830 1334-9090 |
| language | English |
| last_indexed | 2024-12-13T02:50:18Z |
| publishDate | 2019-03-01 |
| publisher | Croatian Society of Chemical Engineers |
| record_format | Article |
| series | Kemija u Industriji |
| spelling | doaj.art-901e3b4289164591925c81936f12e2d92022-12-22T00:02:04ZengCroatian Society of Chemical EngineersKemija u Industriji0022-98301334-90902019-03-01683-49710310.15255/KUI.2018.034Amino Acids Composition and Antioxidant Activity of Selected Mushrooms from Bosnia and HerzegovinaMirsada Salihović0Aida Šapčanin1Ekrem Pehlić2Alija Uzunović3Selma Špirtović-Halilović4Melita Huremović5Faculty of Pharmacy, University of Sarajevo, Zmaja od Bosne 8, 71 000 Sarajevo, Bosnia and HerzegovinaFaculty of Pharmacy, University of Sarajevo, Zmaja od Bosne 8, 71 000 Sarajevo, Bosnia and HerzegovinaFaculty of Health Sciences, University of Bihać, Hrvatskog trolista 4, 77 000 Bihać, Bosnia and HerzegovinaAgency for Medicinal Products and Medical Devices, Titova 9, 71 000, Sarajevo, Bosnia and HerzegovinaFaculty of Pharmacy, University of Sarajevo, Zmaja od Bosne 8, 71 000 Sarajevo, Bosnia and HerzegovinaFaculty of Science, University of Tuzla, Univerzitetska 4, 75 000 Tuzla, Bosnia and HerzegovinaMany studies highlight the health benefits of mushrooms, which are consequently becoming more and more interesting for researchers. The content of amino acids (AA), total phenolic content (TPC), and antioxidative activity (AAc) were determined in wild as well as cultivated mushrooms. The AA included: L-tryptophan (Trp), L-arginine (Arg), L-cysteine (Cys), L-methionine (Met), L-alanine (Ala), L-phenylalanine (Phe), L-lysine (Lys), L-valine (Val), L-glycine (Gly), and L-leucine (Leu). The wild mushrooms: Lactarius piperatus, Amanita caesarea, Lactarius deliciosus, Lycoperdon pyriforme, Macrolepiota procera, and cultivated mushrooms: Agaricus bisporus, Boletus aestivalis, Cantharellus cibarius, Pleurotus ostreatus, and Agaricus bisporus var. avellaneus were investigated in this study. The AA was determined by HPTLC method and quantified with a Camag TLC scanner and WINCAT software by scanning the plates at 540 nm. The TPC was spectrophotometrically estimated as gallic acid equivalents/g of fresh weight according to Folin-Ciocalteu’s method. The radical scavenging activity (RSA) of mushroom extracts was determined by DPPH assay. The highest content of Ala, Gly, Phe, Lys, Val, and Leu was found in Pleurotus ostreatus. The total phenolic content (mg GAE/g) in investigated mushrooms ranged from 1.90 to 35.56, and the % RSA ranged from 43.88 to 90.17. This study promotes the consumption of food rich in bioactive compounds, mushrooms being among such food. Further research on mushrooms from Bosnia and Herzegovina and their benefits in the overall maintenance of human health and protection from age-related diseases is necessary.http://silverstripe.fkit.hr/kui/assets/Uploads/I-97-103.pdfwild mushroomscultivated mushroomsamino acidsHPTLCantioxidant activity |
| spellingShingle | Mirsada Salihović Aida Šapčanin Ekrem Pehlić Alija Uzunović Selma Špirtović-Halilović Melita Huremović Amino Acids Composition and Antioxidant Activity of Selected Mushrooms from Bosnia and Herzegovina Kemija u Industriji wild mushrooms cultivated mushrooms amino acids HPTLC antioxidant activity |
| title | Amino Acids Composition and Antioxidant Activity of Selected Mushrooms from Bosnia and Herzegovina |
| title_full | Amino Acids Composition and Antioxidant Activity of Selected Mushrooms from Bosnia and Herzegovina |
| title_fullStr | Amino Acids Composition and Antioxidant Activity of Selected Mushrooms from Bosnia and Herzegovina |
| title_full_unstemmed | Amino Acids Composition and Antioxidant Activity of Selected Mushrooms from Bosnia and Herzegovina |
| title_short | Amino Acids Composition and Antioxidant Activity of Selected Mushrooms from Bosnia and Herzegovina |
| title_sort | amino acids composition and antioxidant activity of selected mushrooms from bosnia and herzegovina |
| topic | wild mushrooms cultivated mushrooms amino acids HPTLC antioxidant activity |
| url | http://silverstripe.fkit.hr/kui/assets/Uploads/I-97-103.pdf |
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