Characterization of Effective Parameters on the Synthesized Gold Nanoparticles and Investigating their Antimicrobial Activities Using Aqueous Extract of Hibiscus sabdariffa L.
Background: During the last decade research have been focussed on developing simple, clean, non-toxic, cost effective and ecofriendly protocols for synthesis of nanoparticles. Objective: The current study aim to characterization and biosynthesis of gold nano particles (Au NPs) using calyx aqueous ex...
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| Format: | Article |
| Language: | English |
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Institue of Medicinal Plants, ACECR
2017-12-01
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| Series: | Journal of Medicinal Plants |
| Subjects: | |
| Online Access: | http://jmp.ir/article-1-1635-en.html |
| _version_ | 1819123103216697344 |
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| author | M Mehrzadeh J Valizadeh A Ghasemi |
| author_facet | M Mehrzadeh J Valizadeh A Ghasemi |
| author_sort | M Mehrzadeh |
| collection | DOAJ |
| description | Background: During the last decade research have been focussed on developing simple, clean, non-toxic, cost effective and ecofriendly protocols for synthesis of nanoparticles.
Objective: The current study aim to characterization and biosynthesis of gold nano particles (Au NPs) using calyx aqueous extract of Hibiscus sabdariffa L. and study of their antimicrobial activity.
Methods: Gold nano particles (AuNPs) were biosynthesized by using HAuCl4.3H2O and the calyx aqueous extract of Hibiscus sabdariffa L. In order to gain the best AuNPs with a uniform shape and size, parameters affecting on synthesis such as: volume of plant extract, pH of the reaction, concentration of HAuCl4.3H2O solution, time of reaction were investigated and optimized by UV-Vis spectrophotometry. Moreover, transmission electron microscopy (TEM) and X-ray diffraction (XRD) were used for further characterization of the nanoparticles. Finally, the antibacterial properties of gold nanoparticles were evaluated by disc diffusion method.
Results: The results of transmission electron image identified the formation of spherical nanoparticles with a size range of 5-10nm. A sharp peak with a maximum absorbance of 570nm was observed which is the characteristic wavelength of gold nanoparticles. The highest inhibition were obtained with the gold nanoparticles against Hafnia alvei bacteria.
Conclusion: In this study, the gold nanoparticles synthesized by calyx aqueous extract of
H. sabdariffa L. and they have relatively good antibacterial activity against Gram-negative bacteria. |
| first_indexed | 2024-12-22T07:03:01Z |
| format | Article |
| id | doaj.art-901f76299fd1440db8f8f491d803ac2f |
| institution | Directory Open Access Journal |
| issn | 2717-204X 2717-2058 |
| language | English |
| last_indexed | 2024-12-22T07:03:01Z |
| publishDate | 2017-12-01 |
| publisher | Institue of Medicinal Plants, ACECR |
| record_format | Article |
| series | Journal of Medicinal Plants |
| spelling | doaj.art-901f76299fd1440db8f8f491d803ac2f2022-12-21T18:34:44ZengInstitue of Medicinal Plants, ACECRJournal of Medicinal Plants2717-204X2717-20582017-12-011664107122Characterization of Effective Parameters on the Synthesized Gold Nanoparticles and Investigating their Antimicrobial Activities Using Aqueous Extract of Hibiscus sabdariffa L.M Mehrzadeh0J Valizadeh1A Ghasemi2 University of Sistanand & Baluchestan, Zahedan University of Sistanand & Baluchestan, Zahedan University of Sistanand & Baluchestan, Zahedan Background: During the last decade research have been focussed on developing simple, clean, non-toxic, cost effective and ecofriendly protocols for synthesis of nanoparticles. Objective: The current study aim to characterization and biosynthesis of gold nano particles (Au NPs) using calyx aqueous extract of Hibiscus sabdariffa L. and study of their antimicrobial activity. Methods: Gold nano particles (AuNPs) were biosynthesized by using HAuCl4.3H2O and the calyx aqueous extract of Hibiscus sabdariffa L. In order to gain the best AuNPs with a uniform shape and size, parameters affecting on synthesis such as: volume of plant extract, pH of the reaction, concentration of HAuCl4.3H2O solution, time of reaction were investigated and optimized by UV-Vis spectrophotometry. Moreover, transmission electron microscopy (TEM) and X-ray diffraction (XRD) were used for further characterization of the nanoparticles. Finally, the antibacterial properties of gold nanoparticles were evaluated by disc diffusion method. Results: The results of transmission electron image identified the formation of spherical nanoparticles with a size range of 5-10nm. A sharp peak with a maximum absorbance of 570nm was observed which is the characteristic wavelength of gold nanoparticles. The highest inhibition were obtained with the gold nanoparticles against Hafnia alvei bacteria. Conclusion: In this study, the gold nanoparticles synthesized by calyx aqueous extract of H. sabdariffa L. and they have relatively good antibacterial activity against Gram-negative bacteria.http://jmp.ir/article-1-1635-en.html<i>hibiscus sabdariffa</i> l.antibacterial activitybiosynthesisgold nanoparticles |
| spellingShingle | M Mehrzadeh J Valizadeh A Ghasemi Characterization of Effective Parameters on the Synthesized Gold Nanoparticles and Investigating their Antimicrobial Activities Using Aqueous Extract of Hibiscus sabdariffa L. Journal of Medicinal Plants <i>hibiscus sabdariffa</i> l. antibacterial activity biosynthesis gold nanoparticles |
| title | Characterization of Effective Parameters on the Synthesized Gold Nanoparticles and Investigating their Antimicrobial Activities Using Aqueous Extract of Hibiscus sabdariffa L. |
| title_full | Characterization of Effective Parameters on the Synthesized Gold Nanoparticles and Investigating their Antimicrobial Activities Using Aqueous Extract of Hibiscus sabdariffa L. |
| title_fullStr | Characterization of Effective Parameters on the Synthesized Gold Nanoparticles and Investigating their Antimicrobial Activities Using Aqueous Extract of Hibiscus sabdariffa L. |
| title_full_unstemmed | Characterization of Effective Parameters on the Synthesized Gold Nanoparticles and Investigating their Antimicrobial Activities Using Aqueous Extract of Hibiscus sabdariffa L. |
| title_short | Characterization of Effective Parameters on the Synthesized Gold Nanoparticles and Investigating their Antimicrobial Activities Using Aqueous Extract of Hibiscus sabdariffa L. |
| title_sort | characterization of effective parameters on the synthesized gold nanoparticles and investigating their antimicrobial activities using aqueous extract of hibiscus sabdariffa l |
| topic | <i>hibiscus sabdariffa</i> l. antibacterial activity biosynthesis gold nanoparticles |
| url | http://jmp.ir/article-1-1635-en.html |
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