Two randomized, controlled studies to assess the efficacy and safety of lotilaner (Credelio™) in preventing Dermacentor reticulatus transmission of Babesia canis to dogs
Abstract Background Dogs worldwide are at risk of Babesia spp. infections. Preventive efficacy of lotilaner tablets (Credelio™, Elanco) against Babesia canis was evaluated in two studies. Methods Sixteen dogs in Study 1 and 12 dogs in Study 2, all seronegative and polymerase chain reaction (PCR) neg...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2017-11-01
|
| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-017-2473-1 |
| _version_ | 1818258039829954560 |
|---|---|
| author | Daniela Cavalleri Martin Murphy Wolfgang Seewald Jason Drake Steve Nanchen |
| author_facet | Daniela Cavalleri Martin Murphy Wolfgang Seewald Jason Drake Steve Nanchen |
| author_sort | Daniela Cavalleri |
| collection | DOAJ |
| description | Abstract Background Dogs worldwide are at risk of Babesia spp. infections. Preventive efficacy of lotilaner tablets (Credelio™, Elanco) against Babesia canis was evaluated in two studies. Methods Sixteen dogs in Study 1 and 12 dogs in Study 2, all seronegative and polymerase chain reaction (PCR) negative for B. canis, were randomized to a sham-treated control group or a lotilaner (20–43 mg/kg) treatment group, administered on Day 0 (Study 1: n = 8/group; Study 2: n = 6/group). Dogs were each infested with 50 Dermacentor reticulatus, a percentage of which (Study 1: 8.0–30.0%; Study 2: 12.2%) were infected with B. canis, in Study 1 on Days 2, 7, 14, 21 and 28, and in Study 2 on Day 28. Ticks were removed and counted on Day 30 in Study 1, and Day 34 in Study 2. Blood was collected for Babesia detection via smear, PCR and immunofluorescence assay (IFA) in Study 1 on Day 2, then approximately weekly through Day 56, and in Study 2 at weekly intervals between Days 28 to 49, and on Days 63 and 91. Additional samples were collected from dogs with body temperature > 39.4 °C (measured three times weekly, from Days 7 to 56 in Study 1 and from Days 35 to 56 in Study 2) and positive for B. canis on blood smear. Dogs with confirmed infections were rescue-treated, removed from the study and, in Study 1, replaced. Results Across both studies B. canis infection of ticks ranged between 8.0–30.0%. In Study 1, all control dogs were positive for B. canis on blood smear and PCR on Day 10 and IFA on Day 21; on Day 21 seven of eight replacement control dogs were B. canis-positive; no replacement dogs were B. canis-positive following tick removal on Day 30. In Study 2, all control dogs were B. canis-positive on Day 56. All lotilaner-treated dogs remained B. canis-negative at all assessments in both studies. Conclusion Lotilaner efficacy was 100% in preventing establishment of B. canis infection, despite post-treatment challenge with infected ticks on Days 2, 7, 14, 21 and 28. |
| first_indexed | 2024-12-12T17:53:13Z |
| format | Article |
| id | doaj.art-901fa562a80d465dbbd17e6ab7d27ead |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-12T17:53:13Z |
| publishDate | 2017-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-901fa562a80d465dbbd17e6ab7d27ead2022-12-22T00:16:46ZengBMCParasites & Vectors1756-33052017-11-011011710.1186/s13071-017-2473-1Two randomized, controlled studies to assess the efficacy and safety of lotilaner (Credelio™) in preventing Dermacentor reticulatus transmission of Babesia canis to dogsDaniela Cavalleri0Martin Murphy1Wolfgang Seewald2Jason Drake3Steve Nanchen4Elanco Animal HealthElanco Animal HealthElanco Animal HealthElanco Animal HealthElanco Animal HealthAbstract Background Dogs worldwide are at risk of Babesia spp. infections. Preventive efficacy of lotilaner tablets (Credelio™, Elanco) against Babesia canis was evaluated in two studies. Methods Sixteen dogs in Study 1 and 12 dogs in Study 2, all seronegative and polymerase chain reaction (PCR) negative for B. canis, were randomized to a sham-treated control group or a lotilaner (20–43 mg/kg) treatment group, administered on Day 0 (Study 1: n = 8/group; Study 2: n = 6/group). Dogs were each infested with 50 Dermacentor reticulatus, a percentage of which (Study 1: 8.0–30.0%; Study 2: 12.2%) were infected with B. canis, in Study 1 on Days 2, 7, 14, 21 and 28, and in Study 2 on Day 28. Ticks were removed and counted on Day 30 in Study 1, and Day 34 in Study 2. Blood was collected for Babesia detection via smear, PCR and immunofluorescence assay (IFA) in Study 1 on Day 2, then approximately weekly through Day 56, and in Study 2 at weekly intervals between Days 28 to 49, and on Days 63 and 91. Additional samples were collected from dogs with body temperature > 39.4 °C (measured three times weekly, from Days 7 to 56 in Study 1 and from Days 35 to 56 in Study 2) and positive for B. canis on blood smear. Dogs with confirmed infections were rescue-treated, removed from the study and, in Study 1, replaced. Results Across both studies B. canis infection of ticks ranged between 8.0–30.0%. In Study 1, all control dogs were positive for B. canis on blood smear and PCR on Day 10 and IFA on Day 21; on Day 21 seven of eight replacement control dogs were B. canis-positive; no replacement dogs were B. canis-positive following tick removal on Day 30. In Study 2, all control dogs were B. canis-positive on Day 56. All lotilaner-treated dogs remained B. canis-negative at all assessments in both studies. Conclusion Lotilaner efficacy was 100% in preventing establishment of B. canis infection, despite post-treatment challenge with infected ticks on Days 2, 7, 14, 21 and 28.http://link.springer.com/article/10.1186/s13071-017-2473-1LotilanerCredelioDermacentor reticulatusBabesia canisDogEfficacy |
| spellingShingle | Daniela Cavalleri Martin Murphy Wolfgang Seewald Jason Drake Steve Nanchen Two randomized, controlled studies to assess the efficacy and safety of lotilaner (Credelio™) in preventing Dermacentor reticulatus transmission of Babesia canis to dogs Parasites & Vectors Lotilaner Credelio Dermacentor reticulatus Babesia canis Dog Efficacy |
| title | Two randomized, controlled studies to assess the efficacy and safety of lotilaner (Credelio™) in preventing Dermacentor reticulatus transmission of Babesia canis to dogs |
| title_full | Two randomized, controlled studies to assess the efficacy and safety of lotilaner (Credelio™) in preventing Dermacentor reticulatus transmission of Babesia canis to dogs |
| title_fullStr | Two randomized, controlled studies to assess the efficacy and safety of lotilaner (Credelio™) in preventing Dermacentor reticulatus transmission of Babesia canis to dogs |
| title_full_unstemmed | Two randomized, controlled studies to assess the efficacy and safety of lotilaner (Credelio™) in preventing Dermacentor reticulatus transmission of Babesia canis to dogs |
| title_short | Two randomized, controlled studies to assess the efficacy and safety of lotilaner (Credelio™) in preventing Dermacentor reticulatus transmission of Babesia canis to dogs |
| title_sort | two randomized controlled studies to assess the efficacy and safety of lotilaner credelio™ in preventing dermacentor reticulatus transmission of babesia canis to dogs |
| topic | Lotilaner Credelio Dermacentor reticulatus Babesia canis Dog Efficacy |
| url | http://link.springer.com/article/10.1186/s13071-017-2473-1 |
| work_keys_str_mv | AT danielacavalleri tworandomizedcontrolledstudiestoassesstheefficacyandsafetyoflotilanercredelioinpreventingdermacentorreticulatustransmissionofbabesiacanistodogs AT martinmurphy tworandomizedcontrolledstudiestoassesstheefficacyandsafetyoflotilanercredelioinpreventingdermacentorreticulatustransmissionofbabesiacanistodogs AT wolfgangseewald tworandomizedcontrolledstudiestoassesstheefficacyandsafetyoflotilanercredelioinpreventingdermacentorreticulatustransmissionofbabesiacanistodogs AT jasondrake tworandomizedcontrolledstudiestoassesstheefficacyandsafetyoflotilanercredelioinpreventingdermacentorreticulatustransmissionofbabesiacanistodogs AT stevenanchen tworandomizedcontrolledstudiestoassesstheefficacyandsafetyoflotilanercredelioinpreventingdermacentorreticulatustransmissionofbabesiacanistodogs |