The Porcine Circovirus Type 1 in Porcine Kidney 15 Cell Line is Not Transferred to Mice Lymphoid Cells after Xenoimplantation into the Peritoneal Cavity
The porcine circovirus type 1 (PCV1) has been identified within lymphoid tissues of experimental infected pigs and suggested to induce an immunosuppressive stage in pigs. The virus does not induce a cytophatic effect in the pig-derived cell line PK-15. Because PCV1 is prevalent in many pig cells and...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2010-09-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/096368910X504441 |
| _version_ | 1811271734198796288 |
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| author | P. Hernández Jáuregui D.V.M., Ph.D. M. Anaya Ruiz F. Romero Pastrana G. Delgado López A. Pimentel Morales E. Tena Betancourt E. Gómez Conde |
| author_facet | P. Hernández Jáuregui D.V.M., Ph.D. M. Anaya Ruiz F. Romero Pastrana G. Delgado López A. Pimentel Morales E. Tena Betancourt E. Gómez Conde |
| author_sort | P. Hernández Jáuregui D.V.M., Ph.D. |
| collection | DOAJ |
| description | The porcine circovirus type 1 (PCV1) has been identified within lymphoid tissues of experimental infected pigs and suggested to induce an immunosuppressive stage in pigs. The virus does not induce a cytophatic effect in the pig-derived cell line PK-15. Because PCV1 is prevalent in many pig cells and tissues, the risk of inducing a viral xenozoonosis by PCV1 was raised for the xenoimplantation of pig cells into human hosts. The present work evaluated if PCV1 is able to replicate in mice tissues after xenoimplantation of PCV1-infected pig cells. Active growing PK-15 cells harboring PCV1 with or without microencapsulation in sodium alginate were implanted into the peritoneal cavity of mice. After 1 month postimplantation in mice, peritoneal macrophages, spleen, and lymph nodes were harvested and analyzed with the polymerase chain reaction technique (PCR). No evidence of circovirus type 1 DNA was detected within the mice tissues. |
| first_indexed | 2024-04-12T22:26:22Z |
| format | Article |
| id | doaj.art-90234f3d4dbe4a2eb9bef73ce50d4639 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-04-12T22:26:22Z |
| publishDate | 2010-09-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-90234f3d4dbe4a2eb9bef73ce50d46392022-12-22T03:14:08ZengSAGE PublishingCell Transplantation0963-68971555-38922010-09-011910.3727/096368910X504441The Porcine Circovirus Type 1 in Porcine Kidney 15 Cell Line is Not Transferred to Mice Lymphoid Cells after Xenoimplantation into the Peritoneal CavityP. Hernández Jáuregui D.V.M., Ph.D.0M. Anaya Ruiz1F. Romero Pastrana2G. Delgado López3A. Pimentel Morales4E. Tena Betancourt5E. Gómez Conde6 Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social (IMSS), Puebla, México Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social (IMSS), Puebla, México Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social (IMSS), Puebla, México Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social (IMSS), Puebla, México Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social (IMSS), Puebla, México Servicio de Cirugía Experimental y Bioterio, Coordinación de Investigación en Salud del IMSS, México D.F, México Unidad Médica de Alta Especialidad CMN MAC IMSS, Puebla, MéxicoThe porcine circovirus type 1 (PCV1) has been identified within lymphoid tissues of experimental infected pigs and suggested to induce an immunosuppressive stage in pigs. The virus does not induce a cytophatic effect in the pig-derived cell line PK-15. Because PCV1 is prevalent in many pig cells and tissues, the risk of inducing a viral xenozoonosis by PCV1 was raised for the xenoimplantation of pig cells into human hosts. The present work evaluated if PCV1 is able to replicate in mice tissues after xenoimplantation of PCV1-infected pig cells. Active growing PK-15 cells harboring PCV1 with or without microencapsulation in sodium alginate were implanted into the peritoneal cavity of mice. After 1 month postimplantation in mice, peritoneal macrophages, spleen, and lymph nodes were harvested and analyzed with the polymerase chain reaction technique (PCR). No evidence of circovirus type 1 DNA was detected within the mice tissues.https://doi.org/10.3727/096368910X504441 |
| spellingShingle | P. Hernández Jáuregui D.V.M., Ph.D. M. Anaya Ruiz F. Romero Pastrana G. Delgado López A. Pimentel Morales E. Tena Betancourt E. Gómez Conde The Porcine Circovirus Type 1 in Porcine Kidney 15 Cell Line is Not Transferred to Mice Lymphoid Cells after Xenoimplantation into the Peritoneal Cavity Cell Transplantation |
| title | The Porcine Circovirus Type 1 in Porcine Kidney 15 Cell Line is Not Transferred to Mice Lymphoid Cells after Xenoimplantation into the Peritoneal Cavity |
| title_full | The Porcine Circovirus Type 1 in Porcine Kidney 15 Cell Line is Not Transferred to Mice Lymphoid Cells after Xenoimplantation into the Peritoneal Cavity |
| title_fullStr | The Porcine Circovirus Type 1 in Porcine Kidney 15 Cell Line is Not Transferred to Mice Lymphoid Cells after Xenoimplantation into the Peritoneal Cavity |
| title_full_unstemmed | The Porcine Circovirus Type 1 in Porcine Kidney 15 Cell Line is Not Transferred to Mice Lymphoid Cells after Xenoimplantation into the Peritoneal Cavity |
| title_short | The Porcine Circovirus Type 1 in Porcine Kidney 15 Cell Line is Not Transferred to Mice Lymphoid Cells after Xenoimplantation into the Peritoneal Cavity |
| title_sort | porcine circovirus type 1 in porcine kidney 15 cell line is not transferred to mice lymphoid cells after xenoimplantation into the peritoneal cavity |
| url | https://doi.org/10.3727/096368910X504441 |
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