A rapid analysis of Bisphenol A using MISPE coupled with HPLC-FLD in tissues of food-producing animals
Bisphenol A (BPA) is a highly-produced organic compound of anthropogenic origin with a wide-range use and is ubiquitously present in both living organisms and the environment. A previously published analytical method for testing of the free (aglycone) BPA in foodstuffs was simplified and optimized f...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-01-01
|
| Series: | MethodsX |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016121001448 |
| _version_ | 1818968851494207488 |
|---|---|
| author | Vesna Cerkvenik-Flajs Sabina Šturm |
| author_facet | Vesna Cerkvenik-Flajs Sabina Šturm |
| author_sort | Vesna Cerkvenik-Flajs |
| collection | DOAJ |
| description | Bisphenol A (BPA) is a highly-produced organic compound of anthropogenic origin with a wide-range use and is ubiquitously present in both living organisms and the environment. A previously published analytical method for testing of the free (aglycone) BPA in foodstuffs was simplified and optimized for sheep muscle tissue, kidney and liver, by using only a single MISPE purification step allied with HPLC-FLD analysis. The recovery rates and RSD values over a concentration range of 1–10 µg/kg were in the range of 67‒86% and 3‒34%, respectively, while linearity in the matrix, represented by the r2, was ≥0.999. LOD values were 0.5‒1 µg/kg and LOQ values were 1 µg/kg. The analytical method used is a contribution to the field of veterinary toxicology and food testing and also proved to be applicable for other food-producing animal species, e.g. pigs, poultry and freshwater fish. The MISPE sorbent material for testing of BPA was reusable, with up to five re-use cycles without significant loss in performance characteristics. • The paper reports on a rapid determination of free (aglycone) BPA in tissues of food-producing animals by HPLC-FLD and presents a substantial improvement of analytical performance with regard to time- and cost-savings, as well as environmental protection. • MISPE is an advanced analytical technology, and the results proved that in a single and simplified SPE step it had good performance characteristics such as selectivity, recovery and precision, allied with low LOD and LOQ values. • It was proved that MISPE BPA cartridges could be used at least five times without significant loss of methodological recovery and precision. |
| first_indexed | 2024-12-20T14:11:15Z |
| format | Article |
| id | doaj.art-90239d96050a48cab2c6806605aa0b3f |
| institution | Directory Open Access Journal |
| issn | 2215-0161 |
| language | English |
| last_indexed | 2024-12-20T14:11:15Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj.art-90239d96050a48cab2c6806605aa0b3f2022-12-21T19:38:07ZengElsevierMethodsX2215-01612021-01-018101351A rapid analysis of Bisphenol A using MISPE coupled with HPLC-FLD in tissues of food-producing animalsVesna Cerkvenik-Flajs0Sabina Šturm1Corresponding author; University of Ljubljana, Veterinary Faculty, Institute of Pathology, Wild Animals, Fish and Bees, Gerbičeva 60, SI-1000 Ljubljana, SloveniaUniversity of Ljubljana, Veterinary Faculty, Institute of Pathology, Wild Animals, Fish and Bees, Gerbičeva 60, SI-1000 Ljubljana, SloveniaBisphenol A (BPA) is a highly-produced organic compound of anthropogenic origin with a wide-range use and is ubiquitously present in both living organisms and the environment. A previously published analytical method for testing of the free (aglycone) BPA in foodstuffs was simplified and optimized for sheep muscle tissue, kidney and liver, by using only a single MISPE purification step allied with HPLC-FLD analysis. The recovery rates and RSD values over a concentration range of 1–10 µg/kg were in the range of 67‒86% and 3‒34%, respectively, while linearity in the matrix, represented by the r2, was ≥0.999. LOD values were 0.5‒1 µg/kg and LOQ values were 1 µg/kg. The analytical method used is a contribution to the field of veterinary toxicology and food testing and also proved to be applicable for other food-producing animal species, e.g. pigs, poultry and freshwater fish. The MISPE sorbent material for testing of BPA was reusable, with up to five re-use cycles without significant loss in performance characteristics. • The paper reports on a rapid determination of free (aglycone) BPA in tissues of food-producing animals by HPLC-FLD and presents a substantial improvement of analytical performance with regard to time- and cost-savings, as well as environmental protection. • MISPE is an advanced analytical technology, and the results proved that in a single and simplified SPE step it had good performance characteristics such as selectivity, recovery and precision, allied with low LOD and LOQ values. • It was proved that MISPE BPA cartridges could be used at least five times without significant loss of methodological recovery and precision.http://www.sciencedirect.com/science/article/pii/S2215016121001448Analysis of BPA using MISPE coupled with HPLC-FLD in tissues of food-producing animals |
| spellingShingle | Vesna Cerkvenik-Flajs Sabina Šturm A rapid analysis of Bisphenol A using MISPE coupled with HPLC-FLD in tissues of food-producing animals MethodsX Analysis of BPA using MISPE coupled with HPLC-FLD in tissues of food-producing animals |
| title | A rapid analysis of Bisphenol A using MISPE coupled with HPLC-FLD in tissues of food-producing animals |
| title_full | A rapid analysis of Bisphenol A using MISPE coupled with HPLC-FLD in tissues of food-producing animals |
| title_fullStr | A rapid analysis of Bisphenol A using MISPE coupled with HPLC-FLD in tissues of food-producing animals |
| title_full_unstemmed | A rapid analysis of Bisphenol A using MISPE coupled with HPLC-FLD in tissues of food-producing animals |
| title_short | A rapid analysis of Bisphenol A using MISPE coupled with HPLC-FLD in tissues of food-producing animals |
| title_sort | rapid analysis of bisphenol a using mispe coupled with hplc fld in tissues of food producing animals |
| topic | Analysis of BPA using MISPE coupled with HPLC-FLD in tissues of food-producing animals |
| url | http://www.sciencedirect.com/science/article/pii/S2215016121001448 |
| work_keys_str_mv | AT vesnacerkvenikflajs arapidanalysisofbisphenolausingmispecoupledwithhplcfldintissuesoffoodproducinganimals AT sabinasturm arapidanalysisofbisphenolausingmispecoupledwithhplcfldintissuesoffoodproducinganimals AT vesnacerkvenikflajs rapidanalysisofbisphenolausingmispecoupledwithhplcfldintissuesoffoodproducinganimals AT sabinasturm rapidanalysisofbisphenolausingmispecoupledwithhplcfldintissuesoffoodproducinganimals |