Identity and function of an essential nitrogen ligand of the nitrogenase cofactor biosynthesis protein NifB

NifB is a radical SAM enzyme involved in the biosynthesis of the Mo-nitrogenase cofactor, which is responsible for the ambient conversion of N2 to NH3. Here, the authors identify and uncover the function of a His43 residue as an essential nitrogen ligand of NifB in cofactor biosynthesis.

Bibliographic Details
Main Authors: Lee A. Rettberg, Jarett Wilcoxen, Andrew J. Jasniewski, Chi Chung Lee, Kazuki Tanifuji, Yilin Hu, R. David Britt, Markus W. Ribbe
Format: Article
Language:English
Published: Nature Portfolio 2020-04-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-15627-9
Description
Summary:NifB is a radical SAM enzyme involved in the biosynthesis of the Mo-nitrogenase cofactor, which is responsible for the ambient conversion of N2 to NH3. Here, the authors identify and uncover the function of a His43 residue as an essential nitrogen ligand of NifB in cofactor biosynthesis.
ISSN:2041-1723