Taxonomic Characterization and Secondary Metabolite Profiling of Aspergillus Section Aspergillus Contaminating Feeds and Feedstuffs
Xerophilic fungal species of the genus Aspergillus are economically highly relevant due to their ability to grow on low water activity substrates causing spoilage of stored goods and animal feeds. These fungi can synthesize a variety of secondary metabolites, many of which show animal toxicity, crea...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-09-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/7/9/3512 |
| _version_ | 1828117529948585984 |
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| author | Mariana Greco Minna Kemppainen Graciela Pose Alejandro Pardo |
| author_facet | Mariana Greco Minna Kemppainen Graciela Pose Alejandro Pardo |
| author_sort | Mariana Greco |
| collection | DOAJ |
| description | Xerophilic fungal species of the genus Aspergillus are economically highly relevant due to their ability to grow on low water activity substrates causing spoilage of stored goods and animal feeds. These fungi can synthesize a variety of secondary metabolites, many of which show animal toxicity, creating a health risk for food production animals and to humans as final consumers, respectively. Animal feeds used for rabbit, chinchilla and rainbow trout production in Argentina were analysed for the presence of xerophilic Aspergillus section Aspergillus species. High isolation frequencies (>60%) were detected in all the studied rabbit and chinchilla feeds, while the rainbow trout feeds showed lower fungal charge (25%). These section Aspergillus contaminations comprised predominantly five taxa. Twenty isolates were subjected to taxonomic characterization using both ascospore SEM micromorphology and two independent DNA loci sequencing. The secondary metabolite profiles of the isolates were determined qualitatively by HPLC-MS. All the isolates produced neoechinulin A, 17 isolates were positive for cladosporin and echinulin, and 18 were positive for neoechinulin B. Physcion and preechinulin were detected in a minor proportion of the isolates. This is the first report describing the detailed species composition and the secondary metabolite profiles of Aspergillus section Aspergillus contaminating animal feeds. |
| first_indexed | 2024-04-11T13:16:06Z |
| format | Article |
| id | doaj.art-902ace4576ad42178c74544eb8d34140 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T13:16:06Z |
| publishDate | 2015-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-902ace4576ad42178c74544eb8d341402022-12-22T04:22:24ZengMDPI AGToxins2072-66512015-09-01793512353710.3390/toxins7093512toxins7093512Taxonomic Characterization and Secondary Metabolite Profiling of Aspergillus Section Aspergillus Contaminating Feeds and FeedstuffsMariana Greco0Minna Kemppainen1Graciela Pose2Alejandro Pardo3Laboratorio de Micología Molecular, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal 1876, ArgentinaLaboratorio de Micología Molecular, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal 1876, ArgentinaConsejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires 1033, ArgentinaLaboratorio de Micología Molecular, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal 1876, ArgentinaXerophilic fungal species of the genus Aspergillus are economically highly relevant due to their ability to grow on low water activity substrates causing spoilage of stored goods and animal feeds. These fungi can synthesize a variety of secondary metabolites, many of which show animal toxicity, creating a health risk for food production animals and to humans as final consumers, respectively. Animal feeds used for rabbit, chinchilla and rainbow trout production in Argentina were analysed for the presence of xerophilic Aspergillus section Aspergillus species. High isolation frequencies (>60%) were detected in all the studied rabbit and chinchilla feeds, while the rainbow trout feeds showed lower fungal charge (25%). These section Aspergillus contaminations comprised predominantly five taxa. Twenty isolates were subjected to taxonomic characterization using both ascospore SEM micromorphology and two independent DNA loci sequencing. The secondary metabolite profiles of the isolates were determined qualitatively by HPLC-MS. All the isolates produced neoechinulin A, 17 isolates were positive for cladosporin and echinulin, and 18 were positive for neoechinulin B. Physcion and preechinulin were detected in a minor proportion of the isolates. This is the first report describing the detailed species composition and the secondary metabolite profiles of Aspergillus section Aspergillus contaminating animal feeds.http://www.mdpi.com/2072-6651/7/9/3512animal feed spoilagexerophilic speciesteleomorphsmycotoxinsscanning electron microscopy |
| spellingShingle | Mariana Greco Minna Kemppainen Graciela Pose Alejandro Pardo Taxonomic Characterization and Secondary Metabolite Profiling of Aspergillus Section Aspergillus Contaminating Feeds and Feedstuffs Toxins animal feed spoilage xerophilic species teleomorphs mycotoxins scanning electron microscopy |
| title | Taxonomic Characterization and Secondary Metabolite Profiling of Aspergillus Section Aspergillus Contaminating Feeds and Feedstuffs |
| title_full | Taxonomic Characterization and Secondary Metabolite Profiling of Aspergillus Section Aspergillus Contaminating Feeds and Feedstuffs |
| title_fullStr | Taxonomic Characterization and Secondary Metabolite Profiling of Aspergillus Section Aspergillus Contaminating Feeds and Feedstuffs |
| title_full_unstemmed | Taxonomic Characterization and Secondary Metabolite Profiling of Aspergillus Section Aspergillus Contaminating Feeds and Feedstuffs |
| title_short | Taxonomic Characterization and Secondary Metabolite Profiling of Aspergillus Section Aspergillus Contaminating Feeds and Feedstuffs |
| title_sort | taxonomic characterization and secondary metabolite profiling of aspergillus section aspergillus contaminating feeds and feedstuffs |
| topic | animal feed spoilage xerophilic species teleomorphs mycotoxins scanning electron microscopy |
| url | http://www.mdpi.com/2072-6651/7/9/3512 |
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