3D-bioprinting of patient-derived cardiac tissue models for studying congenital heart disease
IntroductionCongenital heart disease is the leading cause of death related to birth defects and affects 1 out of every 100 live births. Induced pluripotent stem cell technology has allowed for patient-derived cardiomyocytes to be studied in vitro. An approach to bioengineer these cells into a physio...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Cardiovascular Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcvm.2023.1162731/full |
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| author | Jayne T. Wolfe Wei He Min-Su Kim Huan-Ling Liang Akankshya Shradhanjali Hilda Jurkiewicz Bonnie P. Freudinger Andrew S. Greene John F. LaDisa John F. LaDisa John F. LaDisa John F. LaDisa Lobat Tayebi Michael E. Mitchell Michael E. Mitchell Aoy Tomita-Mitchell Aoy Tomita-Mitchell Aoy Tomita-Mitchell Aoy Tomita-Mitchell Brandon J. Tefft Brandon J. Tefft |
| author_facet | Jayne T. Wolfe Wei He Min-Su Kim Huan-Ling Liang Akankshya Shradhanjali Hilda Jurkiewicz Bonnie P. Freudinger Andrew S. Greene John F. LaDisa John F. LaDisa John F. LaDisa John F. LaDisa Lobat Tayebi Michael E. Mitchell Michael E. Mitchell Aoy Tomita-Mitchell Aoy Tomita-Mitchell Aoy Tomita-Mitchell Aoy Tomita-Mitchell Brandon J. Tefft Brandon J. Tefft |
| author_sort | Jayne T. Wolfe |
| collection | DOAJ |
| description | IntroductionCongenital heart disease is the leading cause of death related to birth defects and affects 1 out of every 100 live births. Induced pluripotent stem cell technology has allowed for patient-derived cardiomyocytes to be studied in vitro. An approach to bioengineer these cells into a physiologically accurate cardiac tissue model is needed in order to study the disease and evaluate potential treatment strategies.MethodsTo accomplish this, we have developed a protocol to 3D-bioprint cardiac tissue constructs comprised of patient-derived cardiomyocytes within a hydrogel bioink based on laminin-521.ResultsCardiomyocytes remained viable and demonstrated appropriate phenotype and function including spontaneous contraction. Contraction remained consistent during 30 days of culture based on displacement measurements. Furthermore, tissue constructs demonstrated progressive maturation based on sarcomere structure and gene expression analysis. Gene expression analysis also revealed enhanced maturation in 3D constructs compared to 2D cell culture.DiscussionThis combination of patient-derived cardiomyocytes and 3D-bioprinting represents a promising platform for studying congenital heart disease and evaluating individualized treatment strategies. |
| first_indexed | 2024-03-13T09:47:38Z |
| format | Article |
| id | doaj.art-902afc0b7b8144269c98f96e25f4f171 |
| institution | Directory Open Access Journal |
| issn | 2297-055X |
| language | English |
| last_indexed | 2024-03-13T09:47:38Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cardiovascular Medicine |
| spelling | doaj.art-902afc0b7b8144269c98f96e25f4f1712023-05-24T22:21:30ZengFrontiers Media S.A.Frontiers in Cardiovascular Medicine2297-055X2023-05-011010.3389/fcvm.2023.116273111627313D-bioprinting of patient-derived cardiac tissue models for studying congenital heart diseaseJayne T. Wolfe0Wei He1Min-Su Kim2Huan-Ling Liang3Akankshya Shradhanjali4Hilda Jurkiewicz5Bonnie P. Freudinger6Andrew S. Greene7John F. LaDisa8John F. LaDisa9John F. LaDisa10John F. LaDisa11Lobat Tayebi12Michael E. Mitchell13Michael E. Mitchell14Aoy Tomita-Mitchell15Aoy Tomita-Mitchell16Aoy Tomita-Mitchell17Aoy Tomita-Mitchell18Brandon J. Tefft19Brandon J. Tefft20Department of Biomedical Engineering, Medical College of Wisconsin & Marquette University, Milwaukee, WI, United StatesDepartment of Biomedical Engineering, Medical College of Wisconsin & Marquette University, Milwaukee, WI, United StatesDepartment of Surgery, Medical College of Wisconsin, Milwaukee, WI, United StatesDepartment of Surgery, Medical College of Wisconsin, Milwaukee, WI, United StatesDepartment of Biomedical Engineering, Medical College of Wisconsin & Marquette University, Milwaukee, WI, United StatesDepartment of Biomedical Engineering, Medical College of Wisconsin & Marquette University, Milwaukee, WI, United StatesEngineering Core, Medical College of Wisconsin, Milwaukee, WI, United StatesThe Jackson Laboratory, Bar Harbor, ME, United StatesDepartment of Biomedical Engineering, Medical College of Wisconsin & Marquette University, Milwaukee, WI, United StatesDepartment of Pediatrics - Section of Cardiology, Children’s Wisconsin, Milwaukee, WI, United StatesThe Herma Heart Institute, Children’s Wisconsin, Milwaukee, WI, United StatesCardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United StatesSchool of Dentistry, Marquette University, Milwaukee, WI, United StatesDepartment of Surgery, Medical College of Wisconsin, Milwaukee, WI, United StatesThe Herma Heart Institute, Children’s Wisconsin, Milwaukee, WI, United StatesDepartment of Biomedical Engineering, Medical College of Wisconsin & Marquette University, Milwaukee, WI, United StatesDepartment of Surgery, Medical College of Wisconsin, Milwaukee, WI, United StatesThe Herma Heart Institute, Children’s Wisconsin, Milwaukee, WI, United StatesCardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United StatesDepartment of Biomedical Engineering, Medical College of Wisconsin & Marquette University, Milwaukee, WI, United StatesCardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United StatesIntroductionCongenital heart disease is the leading cause of death related to birth defects and affects 1 out of every 100 live births. Induced pluripotent stem cell technology has allowed for patient-derived cardiomyocytes to be studied in vitro. An approach to bioengineer these cells into a physiologically accurate cardiac tissue model is needed in order to study the disease and evaluate potential treatment strategies.MethodsTo accomplish this, we have developed a protocol to 3D-bioprint cardiac tissue constructs comprised of patient-derived cardiomyocytes within a hydrogel bioink based on laminin-521.ResultsCardiomyocytes remained viable and demonstrated appropriate phenotype and function including spontaneous contraction. Contraction remained consistent during 30 days of culture based on displacement measurements. Furthermore, tissue constructs demonstrated progressive maturation based on sarcomere structure and gene expression analysis. Gene expression analysis also revealed enhanced maturation in 3D constructs compared to 2D cell culture.DiscussionThis combination of patient-derived cardiomyocytes and 3D-bioprinting represents a promising platform for studying congenital heart disease and evaluating individualized treatment strategies.https://www.frontiersin.org/articles/10.3389/fcvm.2023.1162731/full3D-bioprintinghydrogelinduced pluripotent stem cellcardiomyocytecongenital heart diseasehypoplastic left heart syndrome |
| spellingShingle | Jayne T. Wolfe Wei He Min-Su Kim Huan-Ling Liang Akankshya Shradhanjali Hilda Jurkiewicz Bonnie P. Freudinger Andrew S. Greene John F. LaDisa John F. LaDisa John F. LaDisa John F. LaDisa Lobat Tayebi Michael E. Mitchell Michael E. Mitchell Aoy Tomita-Mitchell Aoy Tomita-Mitchell Aoy Tomita-Mitchell Aoy Tomita-Mitchell Brandon J. Tefft Brandon J. Tefft 3D-bioprinting of patient-derived cardiac tissue models for studying congenital heart disease Frontiers in Cardiovascular Medicine 3D-bioprinting hydrogel induced pluripotent stem cell cardiomyocyte congenital heart disease hypoplastic left heart syndrome |
| title | 3D-bioprinting of patient-derived cardiac tissue models for studying congenital heart disease |
| title_full | 3D-bioprinting of patient-derived cardiac tissue models for studying congenital heart disease |
| title_fullStr | 3D-bioprinting of patient-derived cardiac tissue models for studying congenital heart disease |
| title_full_unstemmed | 3D-bioprinting of patient-derived cardiac tissue models for studying congenital heart disease |
| title_short | 3D-bioprinting of patient-derived cardiac tissue models for studying congenital heart disease |
| title_sort | 3d bioprinting of patient derived cardiac tissue models for studying congenital heart disease |
| topic | 3D-bioprinting hydrogel induced pluripotent stem cell cardiomyocyte congenital heart disease hypoplastic left heart syndrome |
| url | https://www.frontiersin.org/articles/10.3389/fcvm.2023.1162731/full |
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