Single garlic oil modulates T cells activation and proinflammatory cytokine in mice with high fat diet
Background: Hyperlipidemia triggers atherosclerosis by involving immune cells, such as T-cells. T-cells plays a role in worsening conditions during a high-fat diet (HFD). Objective: The research aimed to analyze the role of single garlic oil (SGO) on T-cells activation and its proinflammatory cytoki...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-10-01
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| Series: | Journal of Ayurveda and Integrative Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0975947618311173 |
| _version_ | 1818611313529585664 |
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| author | Sri Rahayu Lestari Mochammad Fitri Atho’illah Yuyun Ika Christina Muhaimin Rifa’i |
| author_facet | Sri Rahayu Lestari Mochammad Fitri Atho’illah Yuyun Ika Christina Muhaimin Rifa’i |
| author_sort | Sri Rahayu Lestari |
| collection | DOAJ |
| description | Background: Hyperlipidemia triggers atherosclerosis by involving immune cells, such as T-cells. T-cells plays a role in worsening conditions during a high-fat diet (HFD). Objective: The research aimed to analyze the role of single garlic oil (SGO) on T-cells activation and its proinflammatory cytokine expression in HFD mice. Methods: Mice were divided into six groups: ND (normal diet); HFD (high-fat diet without treatment); HFD + Simv (HFD + simvastatin 2.6 mg/kg body weight); and HFD + SGO 1–3 (high-fat diet + single garlic oil in a dose of 12.5, 25, and 50 mg/kg body weight), respectively. Treatments were orally given every day for 45 days. At the end of treatments, lymphocytes were isolated from mice spleen. The relative number of T-cells and proinflammatory cytokines were measured using flow-cytometry and analyzed using one-way ANOVA (p < 0.05). Result: Our result indicated that HFD mice had lower naive T cells (CD4+CD62L+) than normal mice (p < 0.05). SGO treatment in HFD mice increased the relative number of naïve T cells. HFD treatment increased the expression of TNF-α and IFN-γ through NF-κB expression. Furthermore, SGO treatment improved the expression of TNF-α and IFN-γ. Conclusion: Our study suggests that SGO could act as a promising prospect for therapy to improve chronic inflammation in a HFD. |
| first_indexed | 2024-12-16T15:28:21Z |
| format | Article |
| id | doaj.art-902bb7f58d6543ec81947e4ac3dafce4 |
| institution | Directory Open Access Journal |
| issn | 0975-9476 |
| language | English |
| last_indexed | 2024-12-16T15:28:21Z |
| publishDate | 2020-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ayurveda and Integrative Medicine |
| spelling | doaj.art-902bb7f58d6543ec81947e4ac3dafce42022-12-21T22:26:26ZengElsevierJournal of Ayurveda and Integrative Medicine0975-94762020-10-01114414420Single garlic oil modulates T cells activation and proinflammatory cytokine in mice with high fat dietSri Rahayu Lestari0Mochammad Fitri Atho’illah1Yuyun Ika Christina2Muhaimin Rifa’i3Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jalan Semarang, Malang, 65145, East Java, Indonesia; Corresponding author.Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jalan Veteran, Malang, 65145, East Java, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jalan Veteran, Malang, 65145, East Java, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jalan Veteran, Malang, 65145, East Java, Indonesia; Center of Biosystem Study, Brawijaya University, Malang, 65145, East Java, IndonesiaBackground: Hyperlipidemia triggers atherosclerosis by involving immune cells, such as T-cells. T-cells plays a role in worsening conditions during a high-fat diet (HFD). Objective: The research aimed to analyze the role of single garlic oil (SGO) on T-cells activation and its proinflammatory cytokine expression in HFD mice. Methods: Mice were divided into six groups: ND (normal diet); HFD (high-fat diet without treatment); HFD + Simv (HFD + simvastatin 2.6 mg/kg body weight); and HFD + SGO 1–3 (high-fat diet + single garlic oil in a dose of 12.5, 25, and 50 mg/kg body weight), respectively. Treatments were orally given every day for 45 days. At the end of treatments, lymphocytes were isolated from mice spleen. The relative number of T-cells and proinflammatory cytokines were measured using flow-cytometry and analyzed using one-way ANOVA (p < 0.05). Result: Our result indicated that HFD mice had lower naive T cells (CD4+CD62L+) than normal mice (p < 0.05). SGO treatment in HFD mice increased the relative number of naïve T cells. HFD treatment increased the expression of TNF-α and IFN-γ through NF-κB expression. Furthermore, SGO treatment improved the expression of TNF-α and IFN-γ. Conclusion: Our study suggests that SGO could act as a promising prospect for therapy to improve chronic inflammation in a HFD.http://www.sciencedirect.com/science/article/pii/S0975947618311173High-fat dietSingle garlicT-cells |
| spellingShingle | Sri Rahayu Lestari Mochammad Fitri Atho’illah Yuyun Ika Christina Muhaimin Rifa’i Single garlic oil modulates T cells activation and proinflammatory cytokine in mice with high fat diet Journal of Ayurveda and Integrative Medicine High-fat diet Single garlic T-cells |
| title | Single garlic oil modulates T cells activation and proinflammatory cytokine in mice with high fat diet |
| title_full | Single garlic oil modulates T cells activation and proinflammatory cytokine in mice with high fat diet |
| title_fullStr | Single garlic oil modulates T cells activation and proinflammatory cytokine in mice with high fat diet |
| title_full_unstemmed | Single garlic oil modulates T cells activation and proinflammatory cytokine in mice with high fat diet |
| title_short | Single garlic oil modulates T cells activation and proinflammatory cytokine in mice with high fat diet |
| title_sort | single garlic oil modulates t cells activation and proinflammatory cytokine in mice with high fat diet |
| topic | High-fat diet Single garlic T-cells |
| url | http://www.sciencedirect.com/science/article/pii/S0975947618311173 |
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