Comparative Proteomics Reveals the Difference in Root Cold Resistance between <i>Vitis. riparia</i> × <i>V. labrusca</i> and Cabernet Sauvignon in Response to Freezing Temperature
Grapevines, bearing fruit containing large amounts of bioactive metabolites that offer health benefits, are widely cultivated around the world. However, the cold damage incurred when grown outside in extremely low temperatures during the overwintering stage limits the expansion of production. Althou...
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MDPI AG
2022-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/7/971 |
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| author | Sijin Chen Hongyan Su Hua Xing Juan Mao Ping Sun Mengfei Li |
| author_facet | Sijin Chen Hongyan Su Hua Xing Juan Mao Ping Sun Mengfei Li |
| author_sort | Sijin Chen |
| collection | DOAJ |
| description | Grapevines, bearing fruit containing large amounts of bioactive metabolites that offer health benefits, are widely cultivated around the world. However, the cold damage incurred when grown outside in extremely low temperatures during the overwintering stage limits the expansion of production. Although the morphological, biochemical, and molecular levels in different <i>Vitis</i> species exposed to different temperatures have been investigated, differential expression of proteins in roots is still limited. Here, the roots of cold-resistant (<i>Vitis. riparia</i> × <i>V. labrusca</i>, T1) and cold-sensitive varieties (Cabernet Sauvignon, T3) at −4 °C, and also at −15 °C for the former (T2), were measured by iTRAQ-based proteomic analysis. Expression levels of genes encoding candidate proteins were validated by qRT-PCR, and the root activities during different treatments were determined using a triphenyl tetrazolium chloride method. The results show that the root activity of the cold-resistant variety was greater than that of the cold-sensitive variety, and it declined with the decrease in temperature. A total of 25 proteins were differentially co-expressed in T2 vs. T1 and T1 vs. T3, and these proteins were involved in stress response, bio-signaling, metabolism, energy, and translation. The relative expression levels of the 13 selected genes were consistent with their fold-change values of proteins. The signature translation patterns for the roots during spatio-temporal treatments of different varieties at different temperatures provide insight into the differential mechanisms of cold resistance of grapevine. |
| first_indexed | 2024-03-09T11:30:41Z |
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| id | doaj.art-902c2ce971d746209fbe32881b3f22db |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T11:30:41Z |
| publishDate | 2022-04-01 |
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| spelling | doaj.art-902c2ce971d746209fbe32881b3f22db2023-11-30T23:51:38ZengMDPI AGPlants2223-77472022-04-0111797110.3390/plants11070971Comparative Proteomics Reveals the Difference in Root Cold Resistance between <i>Vitis. riparia</i> × <i>V. labrusca</i> and Cabernet Sauvignon in Response to Freezing TemperatureSijin Chen0Hongyan Su1Hua Xing2Juan Mao3Ping Sun4Mengfei Li5State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, ChinaGrapevines, bearing fruit containing large amounts of bioactive metabolites that offer health benefits, are widely cultivated around the world. However, the cold damage incurred when grown outside in extremely low temperatures during the overwintering stage limits the expansion of production. Although the morphological, biochemical, and molecular levels in different <i>Vitis</i> species exposed to different temperatures have been investigated, differential expression of proteins in roots is still limited. Here, the roots of cold-resistant (<i>Vitis. riparia</i> × <i>V. labrusca</i>, T1) and cold-sensitive varieties (Cabernet Sauvignon, T3) at −4 °C, and also at −15 °C for the former (T2), were measured by iTRAQ-based proteomic analysis. Expression levels of genes encoding candidate proteins were validated by qRT-PCR, and the root activities during different treatments were determined using a triphenyl tetrazolium chloride method. The results show that the root activity of the cold-resistant variety was greater than that of the cold-sensitive variety, and it declined with the decrease in temperature. A total of 25 proteins were differentially co-expressed in T2 vs. T1 and T1 vs. T3, and these proteins were involved in stress response, bio-signaling, metabolism, energy, and translation. The relative expression levels of the 13 selected genes were consistent with their fold-change values of proteins. The signature translation patterns for the roots during spatio-temporal treatments of different varieties at different temperatures provide insight into the differential mechanisms of cold resistance of grapevine.https://www.mdpi.com/2223-7747/11/7/971grapevineroot cold resistance<i>V</i><i>itis. riparia</i> × <i>V</i><i>itis. labrusca</i>Cabernet Sauvignonproteomicsgene expression |
| spellingShingle | Sijin Chen Hongyan Su Hua Xing Juan Mao Ping Sun Mengfei Li Comparative Proteomics Reveals the Difference in Root Cold Resistance between <i>Vitis. riparia</i> × <i>V. labrusca</i> and Cabernet Sauvignon in Response to Freezing Temperature Plants grapevine root cold resistance <i>V</i><i>itis. riparia</i> × <i>V</i><i>itis. labrusca</i> Cabernet Sauvignon proteomics gene expression |
| title | Comparative Proteomics Reveals the Difference in Root Cold Resistance between <i>Vitis. riparia</i> × <i>V. labrusca</i> and Cabernet Sauvignon in Response to Freezing Temperature |
| title_full | Comparative Proteomics Reveals the Difference in Root Cold Resistance between <i>Vitis. riparia</i> × <i>V. labrusca</i> and Cabernet Sauvignon in Response to Freezing Temperature |
| title_fullStr | Comparative Proteomics Reveals the Difference in Root Cold Resistance between <i>Vitis. riparia</i> × <i>V. labrusca</i> and Cabernet Sauvignon in Response to Freezing Temperature |
| title_full_unstemmed | Comparative Proteomics Reveals the Difference in Root Cold Resistance between <i>Vitis. riparia</i> × <i>V. labrusca</i> and Cabernet Sauvignon in Response to Freezing Temperature |
| title_short | Comparative Proteomics Reveals the Difference in Root Cold Resistance between <i>Vitis. riparia</i> × <i>V. labrusca</i> and Cabernet Sauvignon in Response to Freezing Temperature |
| title_sort | comparative proteomics reveals the difference in root cold resistance between i vitis riparia i i v labrusca i and cabernet sauvignon in response to freezing temperature |
| topic | grapevine root cold resistance <i>V</i><i>itis. riparia</i> × <i>V</i><i>itis. labrusca</i> Cabernet Sauvignon proteomics gene expression |
| url | https://www.mdpi.com/2223-7747/11/7/971 |
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