GluN2D-containing NMDA receptors-mediate synaptic currents in hippocampal interneurons and pyramidal cells in juvenile mice
The differential regulation of the two major N-methyl-D-aspartate receptor (NMDAR) subunits GluN2A and GluN2B during development in forebrain pyramidal cells has been thoroughly investigated. In contrast, much less is known about the role of GluN2D, which is expressed at low levels and is downregula...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-03-01
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| Series: | Frontiers in Cellular Neuroscience |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fncel.2015.00095/full |
| _version_ | 1818140723221889024 |
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| author | Jakob evon Engelhardt Jakob evon Engelhardt Christina eBocklisch Lars eTönges Anne eHerb Anne eHerb Masayoshi eMishina Hannah eMonyer Hannah eMonyer |
| author_facet | Jakob evon Engelhardt Jakob evon Engelhardt Christina eBocklisch Lars eTönges Anne eHerb Anne eHerb Masayoshi eMishina Hannah eMonyer Hannah eMonyer |
| author_sort | Jakob evon Engelhardt |
| collection | DOAJ |
| description | The differential regulation of the two major N-methyl-D-aspartate receptor (NMDAR) subunits GluN2A and GluN2B during development in forebrain pyramidal cells has been thoroughly investigated. In contrast, much less is known about the role of GluN2D, which is expressed at low levels and is downregulated following the second postnatal week. However, it appears that few cells, presumably interneurons, continue to express GluN2D also in juvenile mice. To investigate which hippocampal cell types express this subunit, we generated transgenic mice with EGFP-tagged GluN2D receptors. The expression of the transgene was confined to hippocampal interneurons, most of which were parvalbumin- and/or somatostatin-positive. Electrophysiological and morphological analyses showed that GluN2D was present mainly in fast spiking basket and axo-axonic cells. Based on pharmacological evidence and electrophysiological analysis of GluN2D knockout mice, we conclude that GluN2D-containing NMDARs mediate synaptic currents in hippocampal interneurons of young and juvenile mice and in CA1 pyramidal neurons of newborn mice. |
| first_indexed | 2024-12-11T10:48:31Z |
| format | Article |
| id | doaj.art-902efc44740143248123f177099b052d |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-11T10:48:31Z |
| publishDate | 2015-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-902efc44740143248123f177099b052d2022-12-22T01:10:24ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022015-03-01910.3389/fncel.2015.00095130083GluN2D-containing NMDA receptors-mediate synaptic currents in hippocampal interneurons and pyramidal cells in juvenile miceJakob evon Engelhardt0Jakob evon Engelhardt1Christina eBocklisch2Lars eTönges3Anne eHerb4Anne eHerb5Masayoshi eMishina6Hannah eMonyer7Hannah eMonyer8German Center for Neurodegenerative DiseasesGerman Cancer Research CenterCharité UniversitätsmedizinUniversity Medicine GöttingenHeidelberg University Medical CenterGerman Cancer Research CenterRitsumeikan UniversityHeidelberg University Medical CenterGerman Cancer Research CenterThe differential regulation of the two major N-methyl-D-aspartate receptor (NMDAR) subunits GluN2A and GluN2B during development in forebrain pyramidal cells has been thoroughly investigated. In contrast, much less is known about the role of GluN2D, which is expressed at low levels and is downregulated following the second postnatal week. However, it appears that few cells, presumably interneurons, continue to express GluN2D also in juvenile mice. To investigate which hippocampal cell types express this subunit, we generated transgenic mice with EGFP-tagged GluN2D receptors. The expression of the transgene was confined to hippocampal interneurons, most of which were parvalbumin- and/or somatostatin-positive. Electrophysiological and morphological analyses showed that GluN2D was present mainly in fast spiking basket and axo-axonic cells. Based on pharmacological evidence and electrophysiological analysis of GluN2D knockout mice, we conclude that GluN2D-containing NMDARs mediate synaptic currents in hippocampal interneurons of young and juvenile mice and in CA1 pyramidal neurons of newborn mice.http://journal.frontiersin.org/Journal/10.3389/fncel.2015.00095/fullAnatomyAxonsDendritesDentate GyrusHippocampusInterneurons |
| spellingShingle | Jakob evon Engelhardt Jakob evon Engelhardt Christina eBocklisch Lars eTönges Anne eHerb Anne eHerb Masayoshi eMishina Hannah eMonyer Hannah eMonyer GluN2D-containing NMDA receptors-mediate synaptic currents in hippocampal interneurons and pyramidal cells in juvenile mice Frontiers in Cellular Neuroscience Anatomy Axons Dendrites Dentate Gyrus Hippocampus Interneurons |
| title | GluN2D-containing NMDA receptors-mediate synaptic currents in hippocampal interneurons and pyramidal cells in juvenile mice |
| title_full | GluN2D-containing NMDA receptors-mediate synaptic currents in hippocampal interneurons and pyramidal cells in juvenile mice |
| title_fullStr | GluN2D-containing NMDA receptors-mediate synaptic currents in hippocampal interneurons and pyramidal cells in juvenile mice |
| title_full_unstemmed | GluN2D-containing NMDA receptors-mediate synaptic currents in hippocampal interneurons and pyramidal cells in juvenile mice |
| title_short | GluN2D-containing NMDA receptors-mediate synaptic currents in hippocampal interneurons and pyramidal cells in juvenile mice |
| title_sort | glun2d containing nmda receptors mediate synaptic currents in hippocampal interneurons and pyramidal cells in juvenile mice |
| topic | Anatomy Axons Dendrites Dentate Gyrus Hippocampus Interneurons |
| url | http://journal.frontiersin.org/Journal/10.3389/fncel.2015.00095/full |
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