Immune Responses Against SARS-CoV-2 WT and Delta Variant in Elderly BNT162b2 Vaccinees
BackgroundResidents of nursing homes are one of the most vulnerable groups during the severe acute syndrome coronavirus 2 (SARS-CoV-2) pandemic. The aim of this study was to characterize cellular and humoral immune responses in >70-year-old participants before vaccination, after first and sec...
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| Format: | Article |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.868361/full |
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| author | Michael Jäger Sissy Therese Sonnleitner Sissy Therese Sonnleitner Stefanie Dichtl Eliott Lafon Gabriel Diem Gernot Walder Cornelia Lass-Flörl Doris Wilflingseder Wilfried Posch |
| author_facet | Michael Jäger Sissy Therese Sonnleitner Sissy Therese Sonnleitner Stefanie Dichtl Eliott Lafon Gabriel Diem Gernot Walder Cornelia Lass-Flörl Doris Wilflingseder Wilfried Posch |
| author_sort | Michael Jäger |
| collection | DOAJ |
| description | BackgroundResidents of nursing homes are one of the most vulnerable groups during the severe acute syndrome coronavirus 2 (SARS-CoV-2) pandemic. The aim of this study was to characterize cellular and humoral immune responses in >70-year-old participants before vaccination, after first and second vaccination with BNT162b2, in contrast to second-dose-vaccinated participants younger than 60 years.MethodsPeripheral blood mononuclear cells of 45 elderly and 40 younger vaccinees were analyzed by IFNγ ELISpot, specific immunoglobulin G antibody titers against SARS-CoV-2 spike protein, and neutralization abilities against SARS-CoV-2 wild-type (WT) and Delta variant (B.1.617.2).ResultsOur results clearly demonstrate a significantly increased T cell response, IgG titers, and neutralization activities against SARS-CoV-2 WT and Delta between first and second vaccination with BNT162b2 in elderly vaccinees, thereby highlighting the importance of the second booster. Interestingly, similar cellular and humoral immune responses against SARS-CoV-2 WT and Delta were found after the second vaccine dose in the young and elderly groups.ConclusionsOur data demonstrate a full picture of cellular and humoral immune responses of BNT162b2-vaccinees in two age cohorts. In all vaccines, SARS-CoV-2 WT-specific antibodies with similar neutralizing activity were detected in all vaccinees. After the second vaccination, neutralization titers against SARS-CoV-2 Delta were impaired in both age groups compared with SARS-CoV-2 WT, thereby emphasizing the need for an additional booster to overcome rising variants of SARS-CoV-2. |
| first_indexed | 2024-12-12T07:42:20Z |
| format | Article |
| id | doaj.art-9030134832484f94bbe519eddb0c8f00 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-12T07:42:20Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-9030134832484f94bbe519eddb0c8f002022-12-22T00:32:44ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-06-011310.3389/fimmu.2022.868361868361Immune Responses Against SARS-CoV-2 WT and Delta Variant in Elderly BNT162b2 VaccineesMichael Jäger0Sissy Therese Sonnleitner1Sissy Therese Sonnleitner2Stefanie Dichtl3Eliott Lafon4Gabriel Diem5Gernot Walder6Cornelia Lass-Flörl7Doris Wilflingseder8Wilfried Posch9Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, AustriaInstitute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, AustriaMedical Laboratory, Department of Virology, Dr. Gernot Walder GmbH, Ausservillgraten, AustriaInstitute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, AustriaInstitute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, AustriaInstitute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, AustriaMedical Laboratory, Department of Virology, Dr. Gernot Walder GmbH, Ausservillgraten, AustriaInstitute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, AustriaInstitute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, AustriaInstitute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, AustriaBackgroundResidents of nursing homes are one of the most vulnerable groups during the severe acute syndrome coronavirus 2 (SARS-CoV-2) pandemic. The aim of this study was to characterize cellular and humoral immune responses in >70-year-old participants before vaccination, after first and second vaccination with BNT162b2, in contrast to second-dose-vaccinated participants younger than 60 years.MethodsPeripheral blood mononuclear cells of 45 elderly and 40 younger vaccinees were analyzed by IFNγ ELISpot, specific immunoglobulin G antibody titers against SARS-CoV-2 spike protein, and neutralization abilities against SARS-CoV-2 wild-type (WT) and Delta variant (B.1.617.2).ResultsOur results clearly demonstrate a significantly increased T cell response, IgG titers, and neutralization activities against SARS-CoV-2 WT and Delta between first and second vaccination with BNT162b2 in elderly vaccinees, thereby highlighting the importance of the second booster. Interestingly, similar cellular and humoral immune responses against SARS-CoV-2 WT and Delta were found after the second vaccine dose in the young and elderly groups.ConclusionsOur data demonstrate a full picture of cellular and humoral immune responses of BNT162b2-vaccinees in two age cohorts. In all vaccines, SARS-CoV-2 WT-specific antibodies with similar neutralizing activity were detected in all vaccinees. After the second vaccination, neutralization titers against SARS-CoV-2 Delta were impaired in both age groups compared with SARS-CoV-2 WT, thereby emphasizing the need for an additional booster to overcome rising variants of SARS-CoV-2.https://www.frontiersin.org/articles/10.3389/fimmu.2022.868361/fullAge-dependent immune responses after BNT162b2SARS-CoV-2 vaccinationT cell immunityneutralizing antibodiesSARS-CoV-2variants of concern (VOCs) |
| spellingShingle | Michael Jäger Sissy Therese Sonnleitner Sissy Therese Sonnleitner Stefanie Dichtl Eliott Lafon Gabriel Diem Gernot Walder Cornelia Lass-Flörl Doris Wilflingseder Wilfried Posch Immune Responses Against SARS-CoV-2 WT and Delta Variant in Elderly BNT162b2 Vaccinees Frontiers in Immunology Age-dependent immune responses after BNT162b2 SARS-CoV-2 vaccination T cell immunity neutralizing antibodies SARS-CoV-2 variants of concern (VOCs) |
| title | Immune Responses Against SARS-CoV-2 WT and Delta Variant in Elderly BNT162b2 Vaccinees |
| title_full | Immune Responses Against SARS-CoV-2 WT and Delta Variant in Elderly BNT162b2 Vaccinees |
| title_fullStr | Immune Responses Against SARS-CoV-2 WT and Delta Variant in Elderly BNT162b2 Vaccinees |
| title_full_unstemmed | Immune Responses Against SARS-CoV-2 WT and Delta Variant in Elderly BNT162b2 Vaccinees |
| title_short | Immune Responses Against SARS-CoV-2 WT and Delta Variant in Elderly BNT162b2 Vaccinees |
| title_sort | immune responses against sars cov 2 wt and delta variant in elderly bnt162b2 vaccinees |
| topic | Age-dependent immune responses after BNT162b2 SARS-CoV-2 vaccination T cell immunity neutralizing antibodies SARS-CoV-2 variants of concern (VOCs) |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.868361/full |
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