Inherent Radiation Sensitivity of Lymphocytes of Triple Negative (TN) and Luminal A: A Comparison Between Patients with Breast Cancer and Normal Individuals as Assayed by the Micronucleus Test
Background: About 83% of patients with breast cancer (BC) undergo radiation therapy. These patients show various degrees of mild to acute reactions during and after the completion of treatment.The aim of this study was to compare inherent radiosensitivity of gamma-irradiated G0-lymphocytes between B...
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| Format: | Article |
| Language: | English |
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Kaviani Breast Disease Institute
2017-03-01
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| Series: | Archives of Breast Cancer |
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| Online Access: | https://archbreastcancer.com/index.php/abc/article/view/118 |
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| author | Hassan Nosrati Hossein Mozdarani Peyman Hadad Ramesh Omranipour Sahar Mozdarani Mohsen Bakhshandeh Sohail Mozdarani Farhad Shahi |
| author_facet | Hassan Nosrati Hossein Mozdarani Peyman Hadad Ramesh Omranipour Sahar Mozdarani Mohsen Bakhshandeh Sohail Mozdarani Farhad Shahi |
| author_sort | Hassan Nosrati |
| collection | DOAJ |
| description | Background: About 83% of patients with breast cancer (BC) undergo radiation therapy. These patients show various degrees of mild to acute reactions during and after the completion of treatment.The aim of this study was to compare inherent radiosensitivity of gamma-irradiated G0-lymphocytes between BC patients and normal individuals using cytokinesis blocked micronucleous assay.
Methods: Three to 4 mL blood was drawn in heparinized syringes from patients and normal individuals. A portion of the sample was irradiated with gamma rays at a dose of 300 cGy. Irradiated and non-irradiated samples were cultured in complete RPMI-1640 culture medium. A standard cytokinesis-blocked micronucleus assay protocol was followed for the preparation of binucleate lymphocytes. Slides were prepared and stained in Giemsa. Thousand binucleate cells were scored for the presence of micronucleus (MN). Data were statistically analyzed using SPSS software.
Results: The results showed that the background frequency of micronuclei in both groups of control and Luminal A (LA) patients was nearly similar and relatively low but was significantly higher in triple negative BC (TNBC) patients Significantly different (P<0.01). The irradiation of lymphocytes led to a high frequency of MN in control and LA patients, relatively higher in LA patients (P<0.001); but the frequency of MN was considerably lower in TNBC patients after irradiation.
Conclusions: The results indicated radio-sensitivity of LA patients but radio-resistance in TNBC patients. This different reaction of lymphocytes of patients with BC might be due to different status of genome instability in these patients. |
| first_indexed | 2024-04-12T14:21:03Z |
| format | Article |
| id | doaj.art-9030bb296a2946c8989dc0a22cb91ad0 |
| institution | Directory Open Access Journal |
| issn | 2383-0433 |
| language | English |
| last_indexed | 2024-04-12T14:21:03Z |
| publishDate | 2017-03-01 |
| publisher | Kaviani Breast Disease Institute |
| record_format | Article |
| series | Archives of Breast Cancer |
| spelling | doaj.art-9030bb296a2946c8989dc0a22cb91ad02022-12-22T03:29:36ZengKaviani Breast Disease InstituteArchives of Breast Cancer2383-04332017-03-014110.19187/abc.20174110-15102Inherent Radiation Sensitivity of Lymphocytes of Triple Negative (TN) and Luminal A: A Comparison Between Patients with Breast Cancer and Normal Individuals as Assayed by the Micronucleus TestHassan Nosrati0Hossein Mozdarani1Peyman Hadad2Ramesh Omranipour3Sahar Mozdarani4Mohsen Bakhshandeh5Sohail Mozdarani6Farhad Shahi7Cancer Research Center, Cancer Institute of Iran, Tehran, IranDepartment of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranRadiation Oncology Research Center, Cancer Institute, Tehran University of Medical Sciences, Tehran, IranCancer Research Center, Cancer Institute of Iran, Tehran, IranCytogenome Medical Genetics Laboratory, Ale-Ahmad Highway, Parvaneh Street, Chamran Medical Building, Tehran, IranDepartment of Radiology Technology, Allied Health School, Shahid Beheshti University of Medical Sciences, Tehran, IranCytogenome Medical Genetics Laboratory, Ale-Ahmad Highway, Parvaneh Street, Chamran Medical Building, Tehran, IranCancer Research Center, Cancer Institute of Iran, Tehran, IranBackground: About 83% of patients with breast cancer (BC) undergo radiation therapy. These patients show various degrees of mild to acute reactions during and after the completion of treatment.The aim of this study was to compare inherent radiosensitivity of gamma-irradiated G0-lymphocytes between BC patients and normal individuals using cytokinesis blocked micronucleous assay. Methods: Three to 4 mL blood was drawn in heparinized syringes from patients and normal individuals. A portion of the sample was irradiated with gamma rays at a dose of 300 cGy. Irradiated and non-irradiated samples were cultured in complete RPMI-1640 culture medium. A standard cytokinesis-blocked micronucleus assay protocol was followed for the preparation of binucleate lymphocytes. Slides were prepared and stained in Giemsa. Thousand binucleate cells were scored for the presence of micronucleus (MN). Data were statistically analyzed using SPSS software. Results: The results showed that the background frequency of micronuclei in both groups of control and Luminal A (LA) patients was nearly similar and relatively low but was significantly higher in triple negative BC (TNBC) patients Significantly different (P<0.01). The irradiation of lymphocytes led to a high frequency of MN in control and LA patients, relatively higher in LA patients (P<0.001); but the frequency of MN was considerably lower in TNBC patients after irradiation. Conclusions: The results indicated radio-sensitivity of LA patients but radio-resistance in TNBC patients. This different reaction of lymphocytes of patients with BC might be due to different status of genome instability in these patients.https://archbreastcancer.com/index.php/abc/article/view/118TNBCLuminal Alymphocytesmicronucleusbreast cancerinherent Radio-sensitivity |
| spellingShingle | Hassan Nosrati Hossein Mozdarani Peyman Hadad Ramesh Omranipour Sahar Mozdarani Mohsen Bakhshandeh Sohail Mozdarani Farhad Shahi Inherent Radiation Sensitivity of Lymphocytes of Triple Negative (TN) and Luminal A: A Comparison Between Patients with Breast Cancer and Normal Individuals as Assayed by the Micronucleus Test Archives of Breast Cancer TNBC Luminal A lymphocytes micronucleus breast cancer inherent Radio-sensitivity |
| title | Inherent Radiation Sensitivity of Lymphocytes of Triple Negative (TN) and Luminal A: A Comparison Between Patients with Breast Cancer and Normal Individuals as Assayed by the Micronucleus Test |
| title_full | Inherent Radiation Sensitivity of Lymphocytes of Triple Negative (TN) and Luminal A: A Comparison Between Patients with Breast Cancer and Normal Individuals as Assayed by the Micronucleus Test |
| title_fullStr | Inherent Radiation Sensitivity of Lymphocytes of Triple Negative (TN) and Luminal A: A Comparison Between Patients with Breast Cancer and Normal Individuals as Assayed by the Micronucleus Test |
| title_full_unstemmed | Inherent Radiation Sensitivity of Lymphocytes of Triple Negative (TN) and Luminal A: A Comparison Between Patients with Breast Cancer and Normal Individuals as Assayed by the Micronucleus Test |
| title_short | Inherent Radiation Sensitivity of Lymphocytes of Triple Negative (TN) and Luminal A: A Comparison Between Patients with Breast Cancer and Normal Individuals as Assayed by the Micronucleus Test |
| title_sort | inherent radiation sensitivity of lymphocytes of triple negative tn and luminal a a comparison between patients with breast cancer and normal individuals as assayed by the micronucleus test |
| topic | TNBC Luminal A lymphocytes micronucleus breast cancer inherent Radio-sensitivity |
| url | https://archbreastcancer.com/index.php/abc/article/view/118 |
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