Combining Flow Cytometry and Metagenomics Improves Recovery of Metagenome-Assembled Genomes in a Cell Culture from Activated Sludge

Combining Flow Cytometry and Metagenomics Improves Recovery of Metagenome-Assembled Genomes in a Cell Culture from Activated Sludge

The recovery of metagenome-assembled genomes is biased towards the most abundant species in a given community. To improve the identification of species, even if only dominant species are recovered, we investigated the integration of flow cytometry cell sorting with bioinformatics tools to recover me...

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Bibliographic Details
Main Authors: Nafi’u Abdulkadir, Joao Pedro Saraiva, Florian Schattenberg, Rodolfo Brizola Toscan, Felipe Borim Correa, Hauke Harms, Susann Müller, Ulisses Nunes da Rocha
Format: Article
Language:English
Published: MDPI AG 2023-01-01
Series:Microorganisms
Subjects:
cell sorting
flow cytometry
metagenome-assembled genomes
metagenomics
Online Access:https://www.mdpi.com/2076-2607/11/1/175

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